Strain Detail Sheet

Strain Name    :

FVB/N-Tg(Zp2*)#Dean Zp2tm1Dean/Mmmh

Stock Number :

034411-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Zp2tm1Dean
Name: targeted mutation 1 Jurrien Dean
Alteration at locus: Knockout
Gene Symbol: Zp2
Name: zona pellucida glycoprotein 2
Chromosome: 7
Alteration at locus: Knockout
Transgene: Tg(Zp2*)#Dean
Name: transgene insertion, Jurrien Dean
Alteration at locus: Transgenic

Genetic Alterations:
Transgene: Random insertion of transgene into genome via microinjection into pronuclei of FVB embryo.
Knockout: Part of exon 1 and exon 2 were replaced by a neomycin cassette.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Females have extremely low to absent fertility. Homozygous males have normal fertility.

Heterozygous phenotype: Normal fertility.

Strain of Origin: FVB/N (transgene), 129/Sv (knockout)

Strain genetic background: Mixed C57BL/6 and FVB

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Reproduction

Strain Origin

Donor: Jurrien Dean, MD, NIDDK/NIH

Primary Reference:

  • Gahlay G; Gauthier L; Baibakov B; Epifano O; Dean J, Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein., Science 2010 Jul 9;329(5988):216-9 (Medline PMID: 20616279)
  • Rankin TL et al., "Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development." Development 2001 Apr;128(7):1119-26 (Medline PMID: 11245577)

Colony and Husbandry Information

Special Considerations

Due to FVB background, mice may occasionally develop vestibular disease; cull all mice that exhibit influence.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034411-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
034411-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/24/2014