Strain Name:
STOCK Zp2tm1Dean Tg(Zp2*)#Dean/Mmmh
Stock Number:
034411-MU
Citation ID:
RRID:MMRRC_034411-MU

Strain Information

Zp2tm1Dean
Name: zona pellucida glycoprotein 2; targeted mutation 1, Jurrien Dean
Synonyms: Zp2tm2Nih, Zp2tm1Nih, Zp2tm
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
NCBI: 22787
Homologene: 48194
Tg(Zp2*)#Dean
Name: transgene insertion, Jurrien Dean
Synonyms: Zp2Mut
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Transgene: Random insertion of transgene into genome via microinjection into pronuclei of FVB embryo.
Knockout: Part of exon 1 and exon 2 were replaced by a neomycin cassette.
Genotype Determination
Phenotype
Homozygous: Females have extremely low to absent fertility. Homozygous males have normal fertility.

Heterozygous: Normal fertility.
Mammalian Phenotype Terms
Allelic Composition: Zp2tm1Dean/Zp2tm1Dean (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CF-1 )

MeSH Terms
  • Acrosome/physiology
  • Acrosome Reaction
  • Animals
  • Cell Adhesion
  • Egg Proteins/genetics
  • Egg Proteins/metabolism
  • Embryo, Mammalian/metabolism
  • Exocytosis
  • Female
  • Fertility
  • Fertilization
  • Ligands
  • Male
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Transgenic
  • Models, Biological
  • Mutant Proteins/metabolism
  • Polysaccharides/metabolism
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Sperm Capacitation
  • Sperm-Ovum Interactions
  • Spermatozoa/metabolism
  • Zona Pellucida/metabolism
  • Zona Pellucida Glycoproteins
  • Zygote/metabolism
  • Egg Proteins/biosynthesis
  • Egg Proteins/physiology
  • Embryonic and Fetal Development
  • Fertility/physiology
  • Fertilization in Vitro
  • Gene Targeting
  • Membrane Glycoproteins/biosynthesis
  • Membrane Glycoproteins/physiology
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Ovarian Follicle/physiology
  • Ovary/pathology
  • Receptors, Cell Surface
  • Zona Pellucida/physiology
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Reproduction
Donor
Jurrien Dean, M.D., National Institute of Diabetes and Digestive and Kidney Diseases/National Institutes of Health
Primary Reference
  • Gahlay G; Gauthier L; Baibakov B; Epifano O; Dean J, Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein., Science 2010 Jul 9;329(5988):216-9 (Medline PMID: 20616279)
  • Rankin TL et al., "Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development." Development 2001 Apr;128(7):1119-26 (Medline PMID: 11245577)

Colony and Husbandry Information

Due to FVB background, mice may occasionally develop vestibular disease; cull all mice that exhibit influence.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034411-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034411-MU-RESUS Litter recovered from cryo-archive $2,697.00 / $5,488.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.