Strain Name    : C57BL/6N-Gt(ROSA)26Sortm1(FLP1)Wtsi/Mmucd

Stock Number : 034413-UCD

Other Names   : FLPeR (flipper)

Gene Information

(provided by MGI)
Target: Gt(ROSA)26Sortm1(FLP1)Wtsi
Name: targeted mutation 1, Wellcome Trust Sanger Institute
Chromosome: 6
Alteration at locus: Knock-In
Target: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Alteration at locus: Knock-In
Genetic Alterations:
FLPeR mice have the FLPe knock-in allele with widespread expression of the FLPe variant of the Saccharomyces cerevisiae FLP1 recombinase gene driven by theGt(ROSA)26Sor promoter.

Genotype Determination:

ES Cell Line:

Phenotype

When bred with mice containing a frt-flanked sequence of interest, FLP-mediated recombination will result in deletion of the frt-flanked sequence(s) in the offspring. This FLP deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLP1-expressing primary cell lines.

Mammalian Phenotype Terms:

Strain of Origin: C57BL/6NTac

Strain genetic background: C57BL/6NTac

Strain Development: ES cell targeting in JM8.F6 ES cells derived from the C57BL/6NTac strain. Upon arrival at MMRRC, the strain was bred to C57BL/6NTac.  Homozygous offspring were mated to establish a homozygous colony for distribution.

Suggested Control Mice: Wild-type littermates

  • Research Tools

Donor: Allan Bradley, Ph.D., Wellcome Trust Sanger Institute

Primary Reference:

Farley FW, Soriano P, Steffen LS, Dymecki SM. Widespread recombinase expression using FLPeR (flipper) mice. Genesis. 2000 Nov- Dec;28(3-4):106-10. (Medline PMID: 11105051 )

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:

MMRRC Breeding System: Random intra-strain mating

Breeding Scheme(s):

  • Homozygous female x Homozygous male

Generation: N2

Overall Breeding Performance: Good

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined

Average litter size: Not specified

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
034413-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034413-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
034413-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.