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Strain Detail Sheet

Published: 06/16/2019
Revised: 04/27/2020

Strain Name:
B6(129S)-Et(icre)21614Rdav/Mmmh
Stock Number:
034580-MU
Citation ID:
RRID:MMRRC_034580-MU
Major Collection:
Neuroscience Blueprint

Strain Information

Gene Details

Et(icre)21614Rdav
Name: enhancer trap 21614, Ron Davis
Type: Allele
Species: Mus musculus (mouse)
Chromosome: UN
Alteration at locus: Transgenic
icre
Name: codon-improved cre recombinase sequence
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Alteration at locus: Transgenic
Genotype Determination

Strain Description

Phenotype
Homozygous: Homozygote phenotype is expected to be similar to hemizygote phenotype.

Hemizygous: Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Specifically for line 21614, the donating investigator reports Cre recombinase activity in brain tissues as:
Expression is constitutive and at the highest level in CA2 and CA3 regions of hippocampus. Lower level of expression is found in most other regions including neocortical layer 4, thalamus, midbrain, etc. In addition, no Cre recombinase activity is reported outside the brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in cre-expressing tissues in the offspring.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Research Tools

Strain Origin

Donor
Ronald L. Davis, Ph.D., The Scripps Research Institute
Paul Overbeek, Ph.D., Baylor College of Medicine
Primary Reference
Additional information regarding this strain can be found at NIH Neuroscience Blueprint Cre Driver Network.
Strain Development
An enhancer trap transgenic approach was used to generate these mice. The Calb1-iCre transgene was designed with a mouse calbindin 1 minimal promoter (~300 bp), an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), and an SV40 late polyA signal. This transgene was injected into C57BL/6J mouse embryos. To establish founder line 21614, founder mice (F0) were bred to B6.R26-lacZ mutant mice (Gt(ROSA)26Sortm1Sor mutant mice backcrossed to C57BL/6N or C57BL/6J for at least 10 generations. The iCre-positive::lacZ-positive transgenic mice (F1) were bred to C57BL/6 mice (either C57BL/6N or C57BL/6J). The iCre-positive::lacZ-negative mice (F2) were bred to C57BL/6 or B6.R26-lacZ mice. The iCre-positive mice (F3) were bred to C57BL/6 or B6.R26-lacZ mice. The resulting iCre-positive:lacZ-positive mice from the F4 generation were used to freeze sperm, and that cryopreserved sperm were sent to the MMRRC. Upon arrival, aliquots of this frozen sperm were used to fertilize oocytes from C57BL/6J inbred females . The resulting mice, now ~N5 onto C57BL/6 genetic background, were bred with C57BL/6J inbred mice, or with wildtype (noncarrier) mice from the colony, or with C57BL/6J-congenic Ai14D reporter mice for at least one generation. At some point, iCre-positive::lacZ-negative::Ai14D-negative mice will be sent to MMRRC-Missouri for public distribution and cryopreservation.

The donating investigator also reports that mice from founder line 21614 were estimated to harbor 5 copies the transgene (assessed by Southern hybridization). The transgene integration site in the genome was not determined.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
034580-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.