Allele Symbol: Adartm1.1Phs
Name: targeted mutation 1.1, Peter H Seeburg
Alteration at locus: Knockout
Gene Symbol: Adar
Name: adenosine deaminase, RNA-specific
Alteration at locus: Knockout
ES Cell Line: R1
Homozygous phenotype: Homozygous embryos die between E11.5 and E12.5 due to a severe liver defect. Expected Mendelian numbers of embryos are recovered at E11.5 and appear normal, however, by E12, embryos exhibit a significant reduction in liver size, a progressively pale yolk sac and developmental retardation. By E12.5, all embryos are dead. By E11.5, fetal livers have few hematopoietic cells, an accumulation of blood in enlarged live spaces, many cells with pyknotic nuclei and few erythroid cells. Hematopoietic tissues including erythroid and myeloid/granuloid progenitors are significantly reduced in the yolk sac, fetal liver and peripheral blood. This mutant mouse strain may be useful in studies of RNA editing and hematopoiesis.
Heterozygous phenotype: Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
Strain of Origin: (129X1/SvJ x 129S1/Sv)F1
Strain genetic background: B6.129(Cg)
Strain Development: The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to mice carrying Tg(CMV-cre)1Cgn, the cre deleter transgene, consequently removing the neomycin cassette and exons 7-9 and leaving a loxP site upstream of exon 10. The strain was transferred to Dr. Nishikura at the Wistar Institute and backcrossed to C57BL/6 for at least 10 generations. During backcrossing the Cre transgene was bred out of the line. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice: Wild-type littermates
Donor: Kazuko Nishikura, Ph.D., The Wistar Institute.
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email firstname.lastname@example.org.
Coat color: Black
MMRRC Breeding System: Backcross or Sib-mating
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||No||No|
|Homozygotes are fertile:|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: Unknown
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.