Strain Name:
B6.129(Cg)-Adartm1.1Phs/KnkMmjax
Stock Number:
034620-JAX
Citation ID:
RRID:MMRRC_034620-JAX

Strain Information

Adartm1.1Phs
Name: adenosine deaminase, RNA-specific; targeted mutation 1.1, Peter H Seeburg
Synonyms: adarf7-9, Adarf
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Adar
Name: adenosine deaminase, RNA-specific
Synonyms: mZaADAR, Adar1p150, Adar1p110, ADAR1
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knockout
NCBI: 56417
HGNC: HGNC:225
Homologene: 9281
Genetic Alterations
A targeting vector containing a loxP-flanked PGKneo cassette was placed in intron 6 and an additional loxP was placed in intron 9.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Homozygous embryos die between E11.5 and E12.5 due to a severe liver defect. Expected Mendelian numbers of embryos are recovered at E11.5 and appear normal, however, by E12, embryos exhibit a significant reduction in liver size, a progressively pale yolk sac and developmental retardation. By E12.5, all embryos are dead. By E11.5, fetal livers have few hematopoietic cells, an accumulation of blood in enlarged live spaces, many cells with pyknotic nuclei and few erythroid cells. Hematopoietic tissues including erythroid and myeloid/granuloid progenitors are significantly reduced in the yolk sac, fetal liver and peripheral blood. This mutant mouse strain may be useful in studies of RNA editing and hematopoiesis.

Heterozygous: Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
MeSH Terms
  • Adenosine Deaminase/deficiency
  • Adenosine Deaminase/genetics
  • Alleles
  • Animals
  • Base Sequence
  • DNA, Complementary/genetics
  • Female
  • Fetal Death/enzymology
  • Fetal Death/genetics
  • Fetal Death/pathology
  • Liver/enzymology
  • Liver/pathology
  • Mice
  • Mice, Knockout
  • Phenotype
  • Pregnancy
  • RNA Editing
  • RNA Precursors/genetics
  • RNA-Binding Proteins
  • Receptor, Serotonin, 5-HT2C/genetics
  • Adenosine Deaminase/metabolism
  • Gene Deletion
  • Gene Expression Regulation
  • Hematopoiesis/genetics
  • Hematopoiesis/immunology
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/immunology
  • Hematopoietic Stem Cells/physiology
  • Inbreeding
  • Interferons/immunology
  • Liver/immunology
  • Mice, Inbred C57BL
  • Signal Transduction
Strain Development
The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to mice carrying Tg(CMV-cre)1Cgn, the cre deleter transgene, consequently removing the neomycin cassette and exons 7-9 and leaving a loxP site upstream of exon 10. The strain was transferred to Dr. Nishikura at the Wistar Institute and backcrossed to C57BL/6 for at least 10 generations. During backcrossing the Cre transgene was bred out of the line. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
Donor
Kazuko Nishikura, Ph.D., The Wistar Institute.
Primary Reference
  • Hartner JC; Schmittwolf C; Kispert A; Muller AM; Higuchi M; Seeburg PH, Liver Disintegration in the Mouse Embryo Caused by Deficiency in the RNA-editing Enzyme ADAR1., J Biol Chem. 2004 Feb 6;279(6):4894-902 (Medline PMID: 14615479)
  • Hartner JC; Walkley CR; Lu J; Orkin SH, ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling., Nat Immunol. 2009 Jan;10(1):109-15 (Medline PMID: 19060901)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Heterozygous female x Heterozygous male
  • C57BL/6 female x Heterozygous male or reciprocal mating
Generation
N10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Unknown
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034620-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
034620-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.