Strain Detail Sheet

Strain Name    :

B6.129(Cg)-Adartm1.1Phs/KnkMmjax

Stock Number :

034620-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Adartm1.1Phs
Name: targeted mutation 1.1, Peter H Seeburg
Alteration at locus: Knockout
Gene Symbol: Adar
Name: adenosine deaminase, RNA-specific
Chromosome: 3
Alteration at locus: Knockout

Genetic Alterations:
A targeting vector containing a loxP-flanked PGKneo cassette was placed in intron 6 and an additional loxP was placed in intron 9.

Genotype Determination:

ES Cell Line: R1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Homozygous embryos die between E11.5 and E12.5 due to a severe liver defect. Expected Mendelian numbers of embryos are recovered at E11.5 and appear normal, however, by E12, embryos exhibit a significant reduction in liver size, a progressively pale yolk sac and developmental retardation. By E12.5, all embryos are dead. By E11.5, fetal livers have few hematopoietic cells, an accumulation of blood in enlarged live spaces, many cells with pyknotic nuclei and few erythroid cells. Hematopoietic tissues including erythroid and myeloid/granuloid progenitors are significantly reduced in the yolk sac, fetal liver and peripheral blood. This mutant mouse strain may be useful in studies of RNA editing and hematopoiesis.

Heterozygous phenotype: Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Strain of Origin: (129X1/SvJ x 129S1/Sv)F1

Strain genetic background: B6.129(Cg)

Strain Development: The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to mice carrying Tg(CMV-cre)1Cgn, the cre deleter transgene, consequently removing the neomycin cassette and exons 7-9 and leaving a loxP site upstream of exon 10. The strain was transferred to Dr. Nishikura at the Wistar Institute and backcrossed to C57BL/6 for at least 10 generations. During backcrossing the Cre transgene was bred out of the line. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation

Strain Origin

Donor: Kazuko Nishikura, Ph.D., The Wistar Institute.

Primary Reference:

  • Hartner JC; Schmittwolf C; Kispert A; Muller AM; Higuchi M; Seeburg PH, Liver Disintegration in the Mouse Embryo Caused by Deficiency in the RNA-editing Enzyme ADAR1., J Biol Chem 2004 Feb 6;279(6):4894-902 (Medline PMID: 14615479)
  • Hartner JC; Walkley CR; Lu J; Orkin SH, ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling., Nat Immunol 2009 Jan;10(1):109-15 (Medline PMID: 19060901)

Colony and Husbandry Information

Special Considerations

While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

  • Heterozygous female x Heterozygous male
  • C57BL/6 female x Heterozygous male or reciprocal mating

Generation: N10

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile:
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: Unknown Unknown

Average litter size: Unknown

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034620-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034620-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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