Strain Detail Sheet

Strain Name    :

B6.Cg-Retntm1Laz Tg(CD68-RETN)#Laz/Mmmh

Stock Number :

034624-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Retntm1Laz
Name: targeted mutation 1, Mitchell A Lazar
Alteration at locus: Targeted Mutation
Gene Symbol: Retn
Name: resistin
Chromosome: 8
Alteration at locus: Targeted Mutation
Transgene: Tg(CD68-RETN)#Laz
Name: transgene insertion, Mitchell A Lazar
Alteration at locus: Transgenic
Promoter: CD68 (Human)
Name: CD68 molecule
Alteration at locus: Transgenic
Transgene: RETN (Human)
Name: resistin
Alteration at locus: Transgenic

Genetic Alterations:
Transgenic mice were derived on the C57BL/6 background with macrophage- specific expression of human resistin using the human CD68 promoter. Several independent lines were generated, 2 of which were established and bred to C57BL/6 Retn-/- mice to generate mice that express human resistin but lack any expression of murine resistin.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Human resistin expressed but no expression of murine resistin.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Retn/Retn Tg(CD68-RETN)#Laz/?
        involves: 129S6/SvEvTac * C57BL/6
  • adipose tissue phenotype
    • abnormal white adipose tissue physiology (MGI Ref ID J:171262)
      • mice exhibit an increase in inflammatory markers (Tnfa, Il6, Il1b, Cxcl15, Cd68) and an accumulation of macrophages in WAT
  • homeostasis/metabolism phenotype
    • enhanced lipolysis (MGI Ref ID J:171262)
    • impaired glucose tolerance (MGI Ref ID J:171262)
      • on a high fat diet
    • increased circulating free fatty acid level (MGI Ref ID J:171262)
      • mice on a high fat diet exhibit significantly increased free fatty acid levels
    • increased circulating glycerol level (MGI Ref ID J:171262)
      • mice on a high fat diet exhibit significantly increased glycerol levels
    • increased circulating insulin level (MGI Ref ID J:171262)
      • after 2-3 weeks on a high fat (60%) diet, male mice exhibit increased serum insulin, but not glucose, levels
    • increased triglyceride level (MGI Ref ID J:171262)
      • mice exhibit a 52% increase in muscle triglyceride levels on a high fat diet
    • insulin resistance (MGI Ref ID J:171262)
      • glucose-infusion rate is 39% lower than controls
      • decrease in insulin sensitivity as determined by insulin tolerance tests
      • decreased glucose disposal rate
      • insulin s timulated glucose uptake in skeletal muscle and white adipose tissue is decreased (respectively 56% and 22%) compared to Retn-/- controls

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Strain Development: Transgenic mice were derived on the C57BL/6 background with macrophage-specific expression of human resistin using the human CD68 promoter. Several independent lines were generated, 2 of which were established and bred to C57BL/6 Retn-/- mice to generate mice that express human resistin but lack any expression of murine resistin.

Suggested Control Mice:

    • Wild-type littermates homozygous for the KO and wild-type for the Tg.

    Research Applications

    • Cardiovascular
    • Cell Biology
    • Diabetes/Obesity
    • Immunology and Inflammation
    • Metabolism
    • Models for Human Disease

    Strain Origin

    Donor: Dr. Mitchell Lazar, University Of Pennsylvania

    Primary Reference:

    Qatanani M, Szwergold NR, Greaves DR, Ahima RS, Lazar MA. Macrophage-derived human resistin exacerbates adipose tissue inflammation and insulin resistance in mice. J Clin Invest. 2009 Mar;119(3):531-9. (Medline PMID: 19188682).

    Colony and Husbandry Information

    Special Considerations

    None.

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

    Appearance

    Coat color: Black

    Other:

    Breeding

    MMRRC Breeding System: Random intra-strain mating

    Breeding Scheme(s):

    • Homozygous KO, Wild-type Tg female x Homozygous KO, Hemizygous Tg 
    • male 

    Generation:

    Overall Breeding Performance: Good

    Reproductive Statistics

    Viability and Fertility:FemaleMale
    Homozygotes are viable: Yes Yes Mice that are homozygous for the KO and hemizygous for the Tg are viable
    Homozygotes are fertile:
    Heterozygotes are fertile: Yes Yes Mice that are homozygous for the KO and hemizygous for the Tg are fertile

    Age Reproductive Decline: Unknown Unknown

    Average litter size: 8

    Recommended wean age: 3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    034624-MU-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.
    034624-MU-SPERMCryo-preserved spermatozoa
    $437.00 / $817.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity.2
    034624-MU-EMBRYOCryo-preserved embryos
    $1,038.00 / $2,621.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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