Strain Name:
STOCK Mir7-2tm1Mtm/Mmjax
Stock Number:
034648-JAX
Citation ID:
RRID:MMRRC_034648-JAX
Major Collection:

Gene Information

Mir7-2tm1Mtm
Name: microRNA 7-2; targeted mutation, Michael McManus
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout-first
Mir7-2
Name: microRNA 7-2
Synonyms: mmu-mir-7a2, Mir7a2, mir 7-2, mmu-mir-7a-2, mmu-mir-7-2, Mirn7-2, mir-7a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout-first
NCBI: 723884
VEGA: 7
Genetic Alterations
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop, with one loxP site placed immediately downstream of the stem loop.
Genotype Determination
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Phenotype
Mice that are homozygous(or heterozygous) for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR-7-2 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR-7-2 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.
Mammalian Phenotype Terms
Allelic Composition: Mir7-2tm1Mtm/Mir7-2tm1Mtm (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Strain Development
The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeric mice were crossed with a mouse of mixed genetic background (which incorporated FVB), then crossed to inbred C57BL/6. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony. Additional information detailing the development of this mirKO mouse can be found here.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Research Tools
Donor
Michael McManus, Ph.D., University of California, San Francisco.
Primary Reference
Park CY, Jeker LT, Carver-Moore K, Oh A, Liu HJ, Cameron R, Richards H, Li Z, Adler D, Yoshinaga Y, Martinez M, Nefadov M, Abbas AK, Weiss A, Lanier LL, de Jong PJ, Bluestone JA, Srivastava D, McManus MT. A Resource for the Conditional Ablation of microRNAs in the Mouse. Cell Rep. 2012 Apr 19;1(4):385-91. (Medline PMID: 22570807).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • C57BL/6 female x Heterozygous male or reciprocal
  • Homozygous female x Homozygous male
Generation
N2
Overall Breeding Performance
Unknown
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Unknown
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034648-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
034648-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.