Strain Name:
B6NCrl.FRA(Cg)-Smpd3fro/Mmnc
Stock Number:
034667-UNC
Citation ID:
RRID:MMRRC_034667-UNC

Strain Information

Smpd3fro
Name: sphingomyelin phosphodiesterase 3, neutral; fragilitas ossium
Synonyms: fro
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Smpd3
Name: sphingomyelin phosphodiesterase 3, neutral
Synonyms: 4631433G07Rik, nSMase2, Nsm2, fro, neutral sphingomyelinase II
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 58994
Homologene: 10260
Genetic Alterations
1.7kb deletion within the Smpd3 gene
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: 1.-Skeletal dysplasia similar to osteogenesis imperfecta. 2.- Spontaneous emphysema.

    Heterozygous: Heterozygous males develop less severe emphysema than homozygous males.
    Mammalian Phenotype Terms
    Allelic Composition: Smpd3fro/Smpd3fro (Genetic Background: B6N.Cg-Smpd3fro )

    Allelic Composition: Smpd3fro/Smpd3fro (Genetic Background: C3H.Cg-Smpd3fro )

    Allelic Composition: Smpd3fro/Smpd3fro (Genetic Background: involves: C3H/HeN * C57BL/6N )

    Allelic Composition: Smpd3fro/Smpd3fro (Genetic Background: Not Specified )

    MeSH Terms
    • Animals
    • Dentinogenesis Imperfecta/enzymology
    • Dentinogenesis Imperfecta/genetics
    • Gene Deletion
    • Mice
    • Mice, Mutant Strains
    • Mutation
    • Osteogenesis Imperfecta/enzymology
    • Osteogenesis Imperfecta/genetics
    • Sphingomyelin Phosphodiesterase
    Strain Development

    Congenic strain

    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6NCrl.FRA(Cg)-Smpd3fro/Mmnc 34667 C57BL/6NJ 129S1/SvImJ 2564 0 18 0
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Developmental Biology
    • Models for Human Disease
    Donor
    Dr. Christophe Poirier, Georgia Health Sciences University
    Primary Reference
    Aubin I; Adams CP; Opsahl S; Septier D; Bishop CE; Auge N; Salvayre R; Negre-Salvayre A; Goldberg M; Guenet JL; Poirier C, A deletion in the gene encoding sphingomyelin phosphodiesterase 3 (Smpd3) results in osteogenesis and dentinogenesis imperfecta in the mouse., Nat Genet 2005 Aug;37(8):803-5 (Medline PMID: 16025116)

    Colony and Husbandry Information

    Heterozygous mice are bred together. Most homozygous die within the first week of life.

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    034667-UNC-HET-F
    034667-UNC-HET-M
    034667-UNC-WT-F
    034667-UNC-WT-M
    Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    Wild Type Female
    Wild Type Male
    $294.00 / $488.00
    Non-Profit / For-Profit
    Per Mouse The mmrrc@med.unc.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    034667-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.