Strain Detail Sheet

Strain Name    :

B6N.129P2-Pde4btm1Mct/Mmucd

Stock Number :

034682-UCD

Other Names   :

PDE4B, B6N.129P2-Pde4btm1Mct/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Pde4btm1Mct
Name: targeted mutation 1, Marco Conti
Alteration at locus: Knockout
Gene Symbol: Pde4b
Name: phosphodiesterase 4B, cAMP specific
Chromosome: 4
Alteration at locus: Knockout

Genetic Alterations:
The gene was disrupted by replacement of a 3 kb region encoding exons 8-10 with a PGK-hprt cassette (in the reverse orientation) via homologous recombination.

Genotype Determination:

ES Cell Line: E14TG2a

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Immune system: impaired neutrophil chemotaxis; impaired neutrophil recruitment;decreased tumor necrosis factor secretion. Psychiatric diseases and striatal function: decreased striatal DA and 5-HT activity associated with decreased prepulse inhibition, decreased baseline motor activity, and an exaggerated locomotor response to amphetamine. PDE4B-null mice were leaner, with lower fat pad weights, smaller adipocytes, and decreased serum leptin levels on both chow and high-fat diets (HFDs).

Heterozygous or Hemizygous phenotype: None.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Pde4btm1Mct/Pde4btm1Mct
        involves: 129P2/OlaHsd
  • cardiovascular system phenotype
    • cardiovascular system phenotype (MGI Ref ID J:96125)
      • mice respond to isoproterenol in the same way as wild-type mice do
Pde4btm1Mct/Pde4btm1Mct
        involves: 129P2/OlaHsd * C57BL/6
  • mortality/aging
    • decreased sensitivity to induced morbidity/mortality (MGI Ref ID J:106828)
      • following LPS-induced shock, mice exhibit less mortality (27% compared to 52%) and die later (3.5 days post-treatment compared to 1.5 days post-treatment) compared to wild-type mice
  • immune system phenotype
    • decreased tumor necrosis factor secretion (MGI Ref ID J:106828)
      • LPS-stimulated TNF-alpha production is decreased to 43.5+/-3.5% of that in control mice
      • thioglycollate-elicited macrophages produce 52.5+/-7.8% of TNF-alpha compared with wild-type cells
      • macrophages do not respond to rolipram inhibition of LPS-stimulated TNF-alpha production
    • impaired neutrophil chemotaxis (MGI Ref ID J:94853)
      • chemotaxis in response to KC and macrophage-inflammatory protein-2 was reduced in homozygous mutant neutrophils compared to wild-type
    • impaired neutrophil recruitment (MGI Ref ID J:94853)
      • 31% reduction in the number of neutrophils that are recruited to the lung following lipopolysaccharide (LPS) inhalation compared to wild-type and homozygous Pde4a mutants
Pde4btm1Mct/Pde4btm1 Mct
        involves: 129X1/SvJ * C57BL/6
  • immune system phenotype
    • decreased tumor necrosis factor secretion (MGI Ref ID J:76862)
      • 90% reduction in TNF-alpha production stimulated by lipo[olysaccharide

Strain of Origin: 129P2/OlaHsd

Strain genetic background: C57BL/6

Strain Development: Mixed 129/Ola (25%) and C57BL/6 (75%) background. Backcrossed 12 times (probably with C57BL/6NTac)in 2003-2004 and 1 time again in 2010 with C57BL/6NTac.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Diabetes/Obesity
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Marco Conti, MD, University of California at San Francisco

Primary Reference:

  • Jin SL; Conti M, Induction of the cyclic nucleotide phosphodiesterase PDE4B is essential for LPS-activated TNF-alpha responses., Proc Natl Acad Sci U S A 2002 May 28;99(11):7628-33. (Medline PMID: 12032334 )
  • Jin SL; Lan L; Zoudilova M; Conti M, Specific role of phosphodiesterase 4B in lipopolysaccharide-induced signaling in mouse macrophages., J Immunol 2005 Aug 1;175(3):1523-31 (Medline PMID: 16034090 )
  • Zhang R; Maratos-Flier E; Flier JS, Reduced adiposity and high-fat diet-induced adipose inflammation in mice deficient for phosphodiesterase 4B., Endocrinology 2009 Jul;150(7):3076-82 (Medline PMID: 19359377 )

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034682-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034682-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
034682-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/23/2014