Strain Name:
B6.Cg-Rec8mei8/JcsMmjax
Stock Number:
034762-JAX
Citation ID:
RRID:MMRRC_034762-JAX
Other Names:
Rec8mei8/Rec8mei8

Strain Information

Rec8mei8
Name: REC8 meiotic recombination protein; mei8, John Schimenti
Synonyms: Rec8-, mei8
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
Rec8
Name: REC8 meiotic recombination protein
Synonyms: mrec, Rec8L1
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 56739
Homologene: 3768
Genetic Alterations
The mei8 mutation was induced in (C57BL/6J x 129S4/SvJae)F1 derived v6.4 ES cells via exposure to ethyl methansulfonate (EMS) during a forward genetic screen for infertility mutations. EMS exposure resulted in a C to T transversion in the terminal amino acid of exon 6 of the Rec8 gene. The mutation alters the corresponding amino acid from cysteine to threonine creating a premature translational stop at amino acid 154 (C154T) and eliminating exons 7 through 20.
Phenotype
Homozygous: Mice that are homozygous for this chemically induced mutation are sterile. Females exhibit ovarian dsygenesis and lack ovarian follicles at reproductive maturity. Testes in affected males are about 50% smaller than wild-type at 5-6 weeks of age due to arrest of spermatogenesis during meiotic prophase I. No postmeiotic spermatids are observed in the seminiferous tubules of 5 week old mice. The underlying cause for arrest in both sexes is failure to repair Spo11-induced meiotic double strand breaks, plus improper synapsis.This mutant mouse strain may be useful in studies of meiosis, synaptonemal complex assembly, double strand break repair and sister chromatid cohesion.
Heterozygous: Heterozygote phenotype is expected to be similar to wild-type phenotype.
Mammalian Phenotype Terms
Allelic Composition: Rec8mei8/Rec8mei8 (Genetic Background: B6.Cg-Rec8mei8 )

Allelic Composition: Rec8mei8/Rec8mei8 (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

MeSH Terms
  • Animals
  • Chromosome Pairing
  • Chromosomes/genetics
  • Codon, Nonsense/genetics
  • Female
  • Infertility/genetics
  • Male
  • Meiotic Prophase I/genetics
  • Mice
  • Mice, Inbred C57BL
  • Nuclear Proteins/antagonists & inhibitors
  • Nuclear Proteins/genetics
  • Oogenesis
  • Ovarian Follicle/cytology
  • Ovarian Follicle/metabolism
  • Phosphoproteins/antagonists & inhibitors
  • Phosphoproteins/genetics
  • Recombination, Genetic/genetics
  • Reproduction/physiology
  • Sister Chromatid Exchange/genetics
  • Spermatogenesis
  • Testis/metabolism
Strain Development
Chimeric males were mated to C57BL/6J females and offspring were backcrossed to C57BL/6J for 9 generations. Males donated to the MMRRC were cryopreserved by sperm.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
John Schimenti , Ph.D., Cornell University
Primary Reference
Bannister LA; Reinholdt LG; Munroe RJ; Schimenti JC, Positional cloning and characterization of mouse mei8, a disrupted allele of the meiotic cohesin Rec8., Genesis. 2004 Oct 27;40(3):184-194 (Medline PMID: 15515002)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

C57BL/6J female x Heterozygous male or recipracol mating
Heterozgyous female x Heterozygous male sibling

Generation
N9+
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034762-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034762-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.