Strain Name:
A.B6-KitW-v/BeiMmjax
Stock Number:
034764-JAX
Citation ID:
RRID:MMRRC_034764-JAX
Other Names:
A/J Wv/+

Gene Information

KitW-v
Name: KIT proto-oncogene receptor tyrosine kinase; viable dominant spotting
Synonyms: KitWv, Wv, W2, Wv, W', Wv
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Spontaneous Mutation
Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Spontaneous Mutation
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Genetic Alterations
A C to T point mutation at nucleotide 2007 results in a threonine to methionine substitution at amino acid 660.
Genotype Determination
Phenotype
Homozygous: A/J Wv/Wv mice are albino so the classic black-eyed white phenotype cannot be assessed.  However, A/J Wv/Wv mice are poorly viable, demonstrating the homozygous mutation has an effect. Because of the reduced viability, we generate (AxB)F1 Wv/Wv mice. These are black-eyed white as expected. We generated these mice to test for mast-cell mediated effects - (AxB)F1 Wv/Wv mice lack mast cells in all tissues examined.
Heterozygous: Normal
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Adoptive Transfer
  • Animals
  • Benzamides
  • Bronchial Hyperreactivity/genetics
  • Bronchial Hyperreactivity/immunology
  • Cell Count
  • Cells, Cultured
  • Female
  • Imatinib Mesylate
  • Lung/drug effects
  • Lung/metabolism
  • Lung/pathology
  • Male
  • Mast Cells/metabolism
  • Mast Cells/pathology
  • Methacholine Chloride/immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Pedigree
  • Piperazines/pharmacology
  • Protein Kinase Inhibitors/pharmacology
  • Proto-Oncogene Proteins c-kit/genetics
  • Pyrimidines/pharmacology
  • Signal Transduction/drug effects
  • Signal Transduction/genetics
  • Spleen/drug effects
  • Spleen/metabolism
  • Spleen/pathology
  • Trachea/drug effects
  • Trachea/metabolism
  • Trachea/pathology
Strain Development
Wv mutation introduced from C57BL/6 into A/J by serial backcross for 10 generations. SNP genotype analysis using Illumina 1440 panel reveals all informative loci are A/J except for those linked to W locus on chr 5.
Suggested Control Mice
Wild-type littermates
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
Donor
David Beier, M.D., Ph.D., Brigham and Women's Hospital.
Primary Reference
Cozzi E, Ackerman KG, Lundequist A, Drazen JM, Boyce JA, Beier DR. The naive airway hyperresponsiveness of the A/J mouse is Kit-mediated. Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12787-92. Epub 2011 Jul 18. (Medline PMID: 21768379).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
White
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

A/J female x Heterozygous male or recipracol mating

Heterozygous female x Heterozygous male

Generation
N10+, strain determined congenic with SNP genotype analysis.
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Unknown
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034764-JAX-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
034764-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.