Strain Detail Sheet

Strain Name    :

STOCK Clic4tm1Jce/Mmnc

Stock Number :

034788-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Clic4tm1Jce
Name: targeted mutation 1, John C Edwards
Alteration at locus: Targeted Mutation
Gene Symbol: Clic4
Name: chloride intracellular channel 4 (mitochondrial)
Chromosome: 4
Alteration at locus: Targeted Mutation

Genetic Alterations:
Deletion of exon 2 with insertion of a neo/cre cassette which deletes itself during passage through sperm.

Genotype Determination:

ES Cell Line: Not specified

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Increased intrauterine death (about 50%), otherwise essentially normal in unstressed lab environment. About 20% of mice develop chronic facial lesions. Decreased response to angiogenic stimuli.

Heterozygous phenotype: No observed phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Clic4tm1Jce/Clic4tm1Jce
        involves: 129S1/Sv * CD-1
  • mortality/aging
    • decreased survivor rate (MGI Ref ID J:146487)
      • fewer than expected homozygous mice are found
  • cardiovascular system phenotype
    • abnormal angiogenesis (MGI Ref ID J:146487)
      • formation of blood filled vessels is decreased by about 60% in matrigel plug assays
      • abnormal physiological neovascularization (MGI Ref ID J:146487)
        • retinal vascular regrowth following high oxygen exposure is reduced compared to controls
      • decreased angiogenesis (MGI Ref ID J:146487)
        • formation of blood filled vessels is decreased by about 60% in matrigel plug assays
    • abnormal retinal vasculature morphology (MGI Ref ID J:146487)
      • at P4 while the area covered by the advancing retinal tree is comparable to wild-type controls tip cells containing large intracellular vacuole are more common and multivacuolated structures are more frequently found behind the front of the expanding vascular tree
      • at P7 the radial fraction of the vascular network to the retinal as a whole is reduced suggesting a delay in vascular development
      • at P21 the retinal vascular tree is less dense and less complex compared to wild-type controls
    • abnormal vascular endothelial cell morphology (MGI Ref ID J:146487)
      • cultured cells show an increase in the number of multivacuolated cells and a decrease in the number of vacuolated cells
    • abnormal vascular endothelial cell physiology (MGI Ref ID J:146487)
      • average pH of large vacuoles is increased indicating a defect in acidification of these vacuoles
  • reproductive system phenotype
    • reduced female fertility (MGI Ref ID J:146487)
      • females show an increase in the occurrence of stillbirths independent of the genotype of the pups
  • growth/size phenotype
    • decreased body weight (MGI Ref ID J:146487)
      • males weigh significantly less than control males
  • vision/eye phenotype
    • abnormal retinal vasculature morphology (MGI Ref ID J:146487)
      • at P4 while the area covered by the advancing retinal tree is comparable to wild-type controls tip cells containing large intracellular vacuole are more common and multivacuolated structures are more frequently found behind the front of the expanding vascular tree
      • at P7 the radial fraction of the vascular network to the retinal as a whole is reduced suggesting a delay in vascular development
      • at P21 the retinal vascular tree is less dense and less complex compared to wild-type controls

Strain of Origin: 129X1/SvJ

Strain genetic background: CD1 outbred strain from Charles River

Strain Development: Original chimeric mice were bred to CD1 mice and then inbred to generate homozygous KOs. The line was re-outbred to commercial CD1 mice twice.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology

Strain Origin

Donor: Dr. John Edwards, University of North Carolina at Chapel Hill

Primary Reference:

  • Ulmasov B; Bruno J; Gordon N; Hartnett ME; Edwards JC, Chloride intracellular channel protein-4 functions in angiogenesis by supporting acidification of vacuoles along the intracellular tubulogenic pathway., Am J Pathol 2009 Mar;174(3):1084-96. (Medline PMID: 19197003)
  • Chalothorn D, Zhang H, Smith JE, Edwards JC, Faber JE. Chloride intracellular channel-4 is a determinant of native collateral formation in skeletal muscle and brain. Circ Res. 2009 Jul 2;105(1):89-98. (Medline PMID: 19478202)

Colony and Husbandry Information

Special Considerations

Mice were outbred to multiple CD1 wild type mice (Charles River). Hets from different mating pairs were bred to generate homozygous KOs and KOs from different parents were crossed to generate mice.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034788-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 03/06/2015