Strain Name:
B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/Mmjax
Stock Number:
034835-JAX
Citation ID:
RRID:MMRRC_034835-JAX
Other Names:
This strain was formerly available as JaxMice stock number 6005. Mo/Hu APPswe E1-2

Gene Information

Prnp
Name: prion protein
Synonyms: Prn-i, Sinc, CD230, PrPC, PrP, Prn-p, PrPSc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19122
HGNC: HGNC:9449
Homologene: 7904
Tg(Prnp-App/APPswe)E1-2Dbo
Name: transgene insertion E1-2, David R Borchelt
Synonyms: E1-2 line, Tg(Prnp-App/APP*Swe)E1-2Dbo, Mo/Hu APPswe line E1-2, Mo/Hu APPswe, line E1-2
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genotype Determination
Phenotype
Homozygous: Not evaluated
Hemizygous: These transgenic mice express a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter. Mice that are hemizygous for the transgene are viable and fertile. More than half of the female hemizygous mice do not survive past 15 months of age. This mutant mouse strain may be useful in studies of Alzheimer's Disease.
MeSH Terms
  • Amyloid beta-Protein Precursor/analysis
  • Amyloid beta-Protein Precursor/genetics
  • Animals
  • Astrocytes/chemistry
  • Brain Chemistry
  • Gene Expression
  • Genetic Vectors/genetics
  • Humans
  • Membrane Proteins/analysis
  • Membrane Proteins/genetics
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Transgenic/genetics
  • Myocardium/chemistry
  • Neurons/chemistry
  • Organ Specificity
  • Presenilin-1
  • Prions/genetics
  • RNA, Messenger/analysis
  • Age Factors
  • Alzheimer Disease/physiopathology
  • Amyloid beta-Peptides/biosynthesis
  • Amyloid beta-Protein Precursor/biosynthesis
  • Brain/metabolism
  • Brain/pathology
  • Brain/physiopathology
  • Cognition/physiology
  • Cross-Sectional Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Immunoblotting
  • Longitudinal Studies
  • Male
  • Maze Learning
  • Memory/physiology
  • Mice, Congenic
  • Models, Animal
  • Mutation
Strain Development
A transgenic construct a chimeric mouse/human amyloid precursor protein (APPswe) under the control of the mouse prion protein promoter was injected into fertilized (C57BL/6J X C3HeJ) F2 mouse eggs. Founder line E1-2 was established. The mice were then backcrossed to C57BL/6J for more than 14 generations.
Suggested Control Mice
Wild-ype littermates
  • Models for Human Disease
  • Neurobiology
Donor
David Borchelt, Ph.D., McKnight Brain Institute, University of Florida
Primary Reference
  • Borchelt DR, Davis J, Fischer M, Lee MK, Slunt HH, Ratovitsky T, Regard J, Copeland NG, Jenkins NA, Sisodia SS, Price DL. A vector for expressing foreign genes in the brains and hearts of transgenic mice. Genet Anal 1996 Dec;13(6):159-63 (Medline PMID: 9117892)
  • Savonenko AV, Xu GM, Price DL, Borchelt DR, Markowska AL. Normal cognitive behavior in two distinct congenic lines of transgenic mice hyperexpressing mutant APP SWE. Neurobiol Dis 2003 Apr;12(3):194-211 (Medline PMID: 12742740)

Colony and Husbandry Information

When maintaining a live colony, these mice are bred as hemizygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034835-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.