Strain Name    : B6.129S6-Acadltm1Uab

Stock Number : 000352-MU

Gene Information

(provided by MGI)
Allele Symbol: Acadltm1Uab
Name: targeted mutation 1, University of Alabama
Chromosome: 1
Alteration at locus: Knockout
Gene Symbol: Acadl
Name: acyl-Coenzyme A dehydrogenase, long-chain
Chromosome: 1
Alteration at locus: Knockout
Genetic Alterations:
Induced duplication of exons 3 & 4, with a neo cassette in intron 4.

Genotype Determination:

ES Cell Line:

Phenotype

Homozygous: Fasting induced hypoketotic-hypoglycemia, fatty liver and heart, cold intolerance, gestational loss, organic aciduria, cardiac hypertrophy, sudden death

Hemizygous: gestational loss

Mammalian Phenotype Terms:
Allelic Composition: Acadltm1Uab/Acadltm1Uab (Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac )

Allelic Composition: Acadltm1Uab/Acadl+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac )

Strain of Origin: 129S6/SvEvTac

Strain genetic background: C57BL/6NTac

Suggested Control Mice: Wild-type littermates

  • Cardiovascular
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Reproduction

Donor: Phillip Wood, D.V.M., Ph.D., University of Alabama at Birmingham.

Primary Reference:

  • Kurtz DM, Rinaldo P, Rhead WJ, Tian L, Millington DS, Vockley J, Hamm DA, Brix AE, Lindsey JR, Pinkert CA, O'Brien WE, Wood PA. Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene reveals crucial roles for fatty acid oxidation. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15592-7. (Medline PMID: 9861014)
  • Guerra C, Koza RA, Walsh K, Kurtz DM, Wood PA, Kozak LP. Abnormal nonshivering thermogenesis in mice with inherited defects of fatty acid oxidation. J Clin Invest. 1998 Nov 1;102(9):1724-31. PMID: 9802886 (Medline PMID: 9802886)
  • Cox KB, Hamm DA, Millington DS, Matern D, Vockley J, Rinaldo P, Pinkert CA, Rhead WJ, Lindsey JR, Wood PA. Gestational, pathologic and biochemical differences between very long-chain acyl-CoA dehydrogenase deficiency and long-chain acyl-CoA dehydrogenase deficiency in the mouse. Hum Mol Genet. 2001 Sep 15;10(19):2069-77. PMID: 11590124 (Medline PMID: 11590124 )

Colony and Husbandry Information

Must be protected from the cold.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Nonagouti

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • C57BL/6 female x Heterozygous male

Generation: N10 As of: 1/2005

Overall Breeding Performance: Excellent

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Unknown Yes
Age Reproductive Decline: Unknown Unknown

Average litter size: 5 - 8

Recommended wean age: 4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000352-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000352-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.