Strain Name:
B6.129S6-Acadltm1Uab/Mmmh
Stock Number:
000352-MU
Citation ID:
RRID:MMRRC_000352-MU
Other Names:
LCAD

Strain Information

Acadltm1Uab
Name: acyl-Coenzyme A dehydrogenase, long-chain; targeted mutation 1, University of Alabama
Synonyms: LCAD-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Acadl
Name: acyl-Coenzyme A dehydrogenase, long-chain
Synonyms: LCAD, C79855
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 11363
HGNC: HGNC:88
Homologene: 37498
Genetic Alterations
Induced duplication of exons 3 & 4, with a neo cassette in intron 4.
Genotype Determination
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Fasting induced hypoketotic-hypoglycemia, fatty liver and heart, cold intolerance, gestational loss, organic aciduria, cardiac hypertrophy, sudden death

Hemizygous: gestational loss
Mammalian Phenotype Terms
Allelic Composition: Acadltm1Uab/Acadltm1Uab (Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac )

Allelic Composition: Acadltm1Uab/Acadl+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac )

MeSH Terms
  • Acyl-CoA Dehydrogenase, Long-Chain/deficiency
  • Acyl-CoA Dehydrogenase, Long-Chain/genetics
  • Acyl-CoA Dehydrogenase, Long-Chain/metabolism
  • Animals
  • Disease Models, Animal
  • Fatty Acids, Nonesterified/blood
  • Fatty Acids, Nonesterified/metabolism
  • Humans
  • Lipid Metabolism, Inborn Errors/enzymology
  • Lipid Metabolism, Inborn Errors/genetics
  • Lipid Metabolism, Inborn Errors/pathology
  • Liver/metabolism
  • Liver/pathology
  • Mice
  • Mice, Knockout
  • Mitochondria, Liver/enzymology
  • Muscle, Skeletal/metabolism
  • Substrate Specificity
  • Acyl-CoA Dehydrogenase
  • Adipose Tissue, Brown/metabolism
  • Body Temperature Regulation/genetics
  • Body Temperature Regulation/physiology
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Cold Temperature
  • Fatty Acids/metabolism
  • Female
  • Gene Expression
  • Ion Channels
  • Male
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Membrane Transport Proteins
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mitochondrial Proteins
  • Oxidation-Reduction
  • Proteins/genetics
  • Receptors, Adrenergic, beta/genetics
  • Receptors, Adrenergic, beta-1/genetics
  • Receptors, Adrenergic, beta-3
  • Uncoupling Protein 1
  • Uncoupling Protein 2
  • Uncoupling Protein 3
  • Carnitine/analogs & derivatives
  • Carnitine/metabolism
  • Litter Size
  • Mitochondria, Heart/enzymology
  • Mitochondria, Muscle/enzymology
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Reproduction
Donor
Phillip Wood, D.V.M., Ph.D., University of Alabama at Birmingham.
Primary Reference
  • Kurtz DM, Rinaldo P, Rhead WJ, Tian L, Millington DS, Vockley J, Hamm DA, Brix AE, Lindsey JR, Pinkert CA, O'Brien WE, Wood PA. Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene reveals crucial roles for fatty acid oxidation. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15592-7. (Medline PMID: 9861014)
  • Guerra C, Koza RA, Walsh K, Kurtz DM, Wood PA, Kozak LP. Abnormal nonshivering thermogenesis in mice with inherited defects of fatty acid oxidation. J Clin Invest. 1998 Nov 1;102(9):1724-31. (Medline PMID: 9802886)
  • Cox KB, Hamm DA, Millington DS, Matern D, Vockley J, Rinaldo P, Pinkert CA, Rhead WJ, Lindsey JR, Wood PA. Gestational, pathologic and biochemical differences between very long-chain acyl-CoA dehydrogenase deficiency and long-chain acyl-CoA dehydrogenase deficiency in the mouse. Hum Mol Genet. 2001 Sep 15;10(19):2069-77. (Medline PMID: 11590124)

Colony and Husbandry Information

Must be protected from the cold.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Nonagouti
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6 female x Heterozygous male
Generation
N10 As of: 1/2005
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Unknown Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
5 - 8
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000352-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000352-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.