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Strain Name:
JU.D2-KitlSl-d/Mmmh
Stock Number:
000359-MU
Citation ID:
RRID:MMRRC_000359-MU
Major Collection:

Strain Information

KitlSl-d
Name: kit ligand; steel Dickie
Synonyms: W/Wv, MGF, Sld, Sld, MgfSl-d, KL
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Spontaneous Mutation
Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, blz, Kitlg
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Spontaneous Mutation
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Genetic Alterations
A 4kb deletion in genomic DNA results in the absence of 241bp of wild type cDNA and the addition of 67bp of novel sequence, a 174bp net loss. The region that is deleted begins 5 amino acids N-terminal to the transmembrane domain and results in termination of the open reading frame after an additional 3 amino acids. The resulting protein is a soluble truncated one, lacking both transmembrane and cytoplasmic domains.
Genotype Determination
Phenotype
Description of White Spotting mutants
Description of Steel Locus mutants

Homozygous mice are black-eyed white; heterozygous mice are black with interspersed white hairs, a very pale belly with a white spot, and a head spot. While homozygotes on the C57BL/6J background die before or shortly after birth, on this background they occasionally survive; surviving homozygous males are fertile.

Familiarly known as Steel-Dickie, this allele was obtained from The Jackson Laboratory, where it occurred and is maintained on a C57BL/6J background. It was used in a study of the impact of the background genome on the size and location of white spots in spotted mice. The belly spots were measured to compare inbred KitlSl-D/+ mice of the JU/CtLm and the C57BL/6J strains. The spots on the JU/CtLm mice were found to be larger and more anterior than those on the C57BL/6J mice, and the JU/CtLm mice had head spots that were lacking in C57BL/6J mice.
Mammalian Phenotype Terms
Allelic Composition: KitlSl-d/KitlSl-d (Genetic Background: C3.D2-KitlSl-d )

Allelic Composition: KitlSl-d/KitlSl-d (Genetic Background: either: DBA/2J or (C57BL/6 x DBA/2)F1 )

Allelic Composition: KitlSl-d/KitlSl-d (Genetic Background: Not Specified )

Allelic Composition: KitlSl-d/Kitl+ (Genetic Background: C3.D2-KitlSl-d )

Allelic Composition: KitlSl-d/Kitl+ (Genetic Background: either: (involves: C57BL/6 * DBA/2J) or (involves: C3H * C57BL/6 * DBA/2J * WC) )

Allelic Composition: KitlSl-d/Kitl+ (Genetic Background: either: DBA/2J or (C57BL/6 x DBA/2)F1 )

Allelic Composition: KitlSl-d/Kitl+ (Genetic Background: involves: DBA/2J )

Allelic Composition: KitlSl/KitlSl-d (Genetic Background: (WC/ReJ KitlSl x B6.D2-KitlSl-d/J)F1-KitlSl/KitlSl-d/J )

Allelic Composition: KitlSl/KitlSl-d (Genetic Background: involves: C3H * C57BL/6 * DBA/2J * WC )

Allelic Composition: KitlSl-d/KitlSl-18J (Genetic Background: involves: C3H/HeJ * C57BL/6J * DBA/2J )

Allelic Composition: KitlSl/KitlSl-d (Genetic Background: involves: C57BL/6 * WC )

MeSH Terms
  • Animals
  • Cell Differentiation/physiology
  • Cell Movement/physiology
  • Cell Survival/physiology
  • Central Nervous System/embryology
  • Cochlea/embryology
  • Embryonic and Fetal Development/genetics
  • Eye/embryology
  • Gene Expression/physiology
  • Genetic Markers/physiology
  • Genotype
  • Harderian Gland/embryology
  • Hematopoietic Cell Growth Factors/genetics
  • Intramolecular Oxidoreductases
  • Isomerases/genetics
  • Melanocytes/physiology
  • Mice
  • Mice, Mutant Strains
  • Molecular Probe Techniques
  • Nucleic Acid Hybridization
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins c-kit
  • Stem Cell Factor
  • Mice, Inbred C57BL
  • Mice, Inbred Strains/genetics
  • Phenotype
  • Skin Pigmentation/genetics
  • Alleles
  • Chromosome Mapping
  • Crosses, Genetic
  • Female
  • Gene Expression Regulation, Developmental
  • Genetic Complementation Test
  • Genetic Linkage
  • Genetic Markers
  • Hypopigmentation/genetics
  • Male
  • Mice, Inbred C3H
  • Receptor, Endothelin B
  • Receptors, Endothelin/chemistry
  • Receptors, Endothelin/deficiency
  • Receptors, Endothelin/genetics
Strain Development
A C57BL/6J-KitlSl-d male mouse was obtained from The Jackson Laboratory. The allele was backcrossed on to JU/CtLm for at least ten generations (and also maintained on a C57BL/6J background). The ventral spotting on the JU/CtLm mouse is located more anteriorly than is the case with the C57BL mouse. The JU/CtLm mouse has a prominent head spot, while the C57BL/6J mouse does not.
Suggested Control Mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Hematopoeisis
  • Pigmentation
  • Mast cell differentiation
  • Reproduction
  • Modifier genes
  • Neural Crest Function
  • Cell Survival
  • Anemia
  • Deafness
  • Brain Development
  • Mast Cells
  • T Cells
  • Reproductive Cells
  • Cell Surface Receptor/Ligand Interactions
  • Cell Migration Patterns
Donor
Dr. ML Lamoreux, Texas A&M University
Primary Reference
  • Steel KP, Davidson DR, Jackson IJ. TRP-2/DT, a new early melanoblast marker, shows that steel growth factor (c-kit ligand) is a survival factor., Development 1992 Aug;115(4):1111-9. (Medline PMID: 1280558)
  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Lamoreux, ML. Pigment cell research 12(6):383-90, 1999. (Medline PMID: 10614578)
  • Rhim H, Dunn KJ, Aronzon A, Mac S, Cheng M, Lamoreux ML, Tilghman SM, Pavan WJ. Spatially restricted hypopigmentation associated with an Ednrbs-modifying locus on mouse chromosome 10. Genome Res. 2000 Jan;10(1):17-29. (Medline PMID: 10645946)

Colony and Husbandry Information

Feed breeders 9% fat diet.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
The homozygote is white and the heterozygote is black with interspersed white hairs, a very pale belly with a white spot, and a head spot.
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous female x Heterozygous male
  • Heterozygous mice to black littermates or backcross to MMRRC:000136
Generation
N10+
Overall Breeding Performance
Good in heterozygotes
Average litter size
8
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000359-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000359-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000359-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.