Strain Name:
B6.129-Sv2atm1Baj/Mmmh
Stock Number:
036049-MU
Citation ID:
RRID:MMRRC_036049-MU
Other Names:
SV2A

Strain Information

Sv2atm1Baj
Name: synaptic vesicle glycoprotein 2a; targeted mutation 1, Sandra M Bajjalieh
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Targeted Mutation
Sv2a
Name: synaptic vesicle glycoprotein 2a
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Targeted Mutation
NCBI: 64051
Homologene: 32237
Genetic Alterations
A neomycin resistance gene replaced a genomic fragment including the exon containing the translation initiation codon. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable encoded protein was present.
Genotype Determination
ES Cell Line
Not Specified
Phenotype
Homozygous: Mice homozygous for the SV2A gene disruption are small, develop severe seizures at ~P7 and generally die by P21.
Mammalian Phenotype Terms
Allelic Composition: Sv2atm1Baj/Sv2atm1Baj (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

Allelic Composition: Sv2atm1Baj/Sv2atm1Baj (Genetic Background: Not Specified )

Allelic Composition: Sv2atm1Baj/Sv2a+ (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Brain/anatomy & histology
  • Endocrine System/abnormalities
  • Genes, Lethal
  • Hippocampus/physiology
  • Homozygote
  • Membrane Glycoproteins/deficiency
  • Membrane Glycoproteins/genetics
  • Mice
  • Mice, Knockout/growth & development
  • Mutagenesis
  • Nerve Tissue Proteins/deficiency
  • Nerve Tissue Proteins/genetics
  • Nervous System Malformations
  • Protein Isoforms
  • Seizures/genetics
  • Synapses/ultrastructure
  • Synaptic Transmission/physiology
  • gamma-Aminobutyric Acid/metabolism
  • Adrenal Medulla/metabolism
  • Adrenal Medulla/secretion
  • Adrenal Medulla/ultrastructure
  • Calcium Signaling/genetics
  • Cell Count
  • Chromaffin Cells/metabolism
  • Chromaffin Cells/secretion
  • Chromaffin Cells/ultrastructure
  • Exocytosis/genetics
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Mice, Knockout
  • Microscopy, Electron
  • Nervous System/metabolism
  • Nervous System/secretion
  • Nervous System/ultrastructure
  • Neurotransmitter Agents/metabolism
  • Neurotransmitter Agents/secretion
  • Presynaptic Terminals/metabolism
  • Presynaptic Terminals/secretion
  • Presynaptic Terminals/ultrastructure
  • Protein Isoforms/genetics
  • Protein Transport/genetics
  • SNARE Proteins
  • Secretory Vesicles/metabolism
  • Secretory Vesicles/secretion
  • Secretory Vesicles/ultrastructure
  • Synaptic Membranes/metabolism
  • Synaptic Membranes/ultrastructure
  • Vesicular Transport Proteins
  • Action Potentials
  • Astrocytes/physiology
  • Calcium/pharmacology
  • Cells, Cultured
  • Evoked Potentials/physiology
  • Genotype
  • Hippocampus/cytology
  • Membrane Glycoproteins/physiology
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins/physiology
  • Neurons/secretion
  • Patch-Clamp Techniques
  • Synaptic Vesicles/secretion
  • Synaptic Vesicles/ultrastructure
Strain Development
The original strain was obtained from germline transmission of the SV2A gene disruption (129SVJ cells) into a C57BL/6 female resulting in a line that was approximately 50% 129, 50% B6.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Neurobiology
Donor
Sandra Bajjalieh, Ph.D., University of Washington
Primary Reference
  • Crowder KM, Gunther JM, Jones TA, Hale BD, Zhang HZ, Peterson MR, Scheller RH, Chavkin C, Bajjalieh SM. Abnormal neurotransmission in mice lacking synaptic vesicle protein 2A (SV2A). Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15268-73. (Medline PMID: 10611374)
  • Xu T, Bajjalieh SM. SV2 modulates the size of the readily releasable pool of secretory vesicles. Nat Cell Biol. 2001 Aug;3(8):691-8. (Medline PMID: 11483953)
  • Custer KL, Austin NS, Sullivan JM, Bajjalieh SM. Synaptic vesicle protein 2 enhances release probability at quiescent synapses. J Neurosci. 2006 Jan 25;26(4):1303-13. (Medline PMID: 16436618)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036049-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.