Allele Symbol: Eya1tm1Rilm
Name: targeted mutation 1, Richard Maas
Alteration at locus: Knockout
Gene Symbol: Eya1
Name: eyes absent 1 homolog (Drosophila)
Alteration at locus: Knockout
ES Cell Line: R1 derived from 129
Eya1-/- mice die perinatally and display a range of anomalies, including cleft palate, reduced head size and a variety of skeletal abnormalities, including in the skull. The cleft palate phenotype differs with background. Eya1-/- in the 129/SV or Balb/C background results in secondary cleft palate whereas Eya1-/- in the C57BL/6J results in a fusion of the palatal shelves with the nasal septum. Eya1 null mice also display renal, otic, thymus, and parathyroid agenesis.Mammalian Phenotype Terms:
Strain of Origin: B6; 129
Strain genetic background: C57BL/6 (N10+)
Strain Development: see alteration
Suggested Control Mice: Wild-type littermates
Donor: Richard Maas, Ph.D., Brigham and Women's Hospital / Harvard Med School
Xu PX, Adams J, Peters H, Brown MC, Heaney S, Maas R. Eya1-deficient mice lack ears and kidneys and show abnormal apoptosis of organ primordia. Nat Genet. 1999 Sep;23(1):113-7. (Medline PMID: 10471511 )
Coat color: Black
MMRRC Breeding System: Inbred
Overall Breeding Performance: Normal when bred Heterozygous x Wildtype.
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||No||No|
|Homozygotes are fertile:||No||No|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||16 weeks||16 weeks|
Average litter size: 6 - 8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.