Strain Detail Sheet

Strain Name    :

B6.129-Eya1tm1Rilm/Mmucd

Stock Number :

000361-UCD

Other Names   :

Eya1 knockout, B6.129-Eya1tm1Rilm/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Eya1tm1Rilm
Name: targeted mutation 1, Richard Maas
Alteration at locus: Knockout
Gene Symbol: Eya1
Name: eyes absent 1 homolog (Drosophila)
Chromosome: 1
Alteration at locus: Knockout

Genetic Alterations:
Isolated Eya1 genomic clones encoding the Eya domain from a 129/SvJ BAC genomic library. Correct targeting resulted in deletion from 305 bp upstream of exon 10 to 1,068 bp downstream of exon 13 in the Eya domain region and replacement with pgk-neo. The targeting construct was electroporated into R1 ES cells.

Genotype Determination:

ES Cell Line: R1 derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Eya1-/- mice die perinatally and display a range of anomalies, including cleft palate, reduced head size and a variety of skeletal abnormalities, including in the skull. The cleft palate phenotype differs with background. Eya1-/- in the 129/SV or Balb/C background results in secondary cleft palate whereas Eya1-/- in the C57BL/6J results in a fusion of the palatal shelves with the nasal septum. Eya1 null mice also display renal, otic, thymus, and parathyroid agenesis.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Eya1tm1Rilm/Eya1+
        either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * BALB/c)
  • hearing/vestibular/ear phenotype
    • abnormal middle ear ossicle morphology (MGI Ref ID J:57313)
      • morphological abnormalities of the ossicles are seen
      • abnormal stapes morphology (MGI Ref ID J:57313)
        • the stapes fails to contact the oval window because of abnormal VIIth cranial nerve placement
    • abnormal vestibular labyrinth (MGI Ref ID J:57313)
      • abnormalities in the vestibular portion of the membranous labyrinth are seen
    • conductive hearing loss (MGI Ref ID J:57313)
      • heterozygotes display some degree of hearing loss in at least one ear associated with abnormalities of the middle ear
      • the stapes failed to contact the oval window because the VIIth cranial nerve passed abnormally between the stapes and the oval window or over the surface of the cochlea under the stapedial artery
    • decreased brainstem auditory evoked potential (MGI Ref ID J:57313)
      • hearing loss was variable from ear to ear in individual animals (one heterozygote had normal hearing in one ear and >70 dB loss in the other); 8 animals had a severe hearing loss (threshold shifted by >50 dB between 15 and 32 kHz), whereas only 2 showed normal hearing
  • renal/urinary system phenotype
    • renal hypoplasia (MGI Ref ID J:57313)
      • unilateral or bilateral kidney hypoplasia was seen in 2 out of 25 mutants
  • skeleton phenotype
    • abnormal middle ear ossicle morphology (MGI Ref ID J:57313)
      • morphological abnormalities of the ossicles are seen
      • abnormal stapes morphology (MGI Ref ID J:57313)
        • the stapes fails to contact the oval window because of abnormal VIIth cranial nerve placement
  • nervous system phenotype
    • abnormal facial nerve morphology (MGI Ref ID J:57313)
      • the VIIth cranial nerve passes abnormally between the stapes and oval window or over the surface of the cochlea
    • abnormal vestibulocochlear nerve morphology (MGI Ref ID J:57313)
      • atrophy of the vestibulocochlear nerve was seen in 2 out of 15 mutants
    • cochlear ganglion degeneration (MGI Ref ID J:57313)
      • atrophy of the spiral ganglion was seen in 2 out of 15 mutants
  • craniofacial phenotype
    • abnormal middle ear ossicle morphology (MGI Ref ID J:57313)
      • morphological abnormalities of the ossicles are seen
      • abnormal stapes morphology (MGI Ref ID J:57313)
        • the stapes fails to contact the oval window because of abnormal VIIth cranial nerve placement
Eya1tm1Rilm/Eya1+
        involves: 129 * C3HeB/FeJ
  • hearing/vestibular/ear phenotype
    • abnormal cochlea morphology (MGI Ref ID J:140027)
      • abnormal orientation of cochlear hair cell stereociliary bundles (MGI Ref ID J:140027)
        • adult mice exhibit short and disorganized stereocilia bundles
      • decreased cochlear coiling (MGI Ref ID J:140027)
        • nine of 22 mice show shortened or/and malformed cochlea at E18.5
        • one ear retained only about one-half turn and another ear retained about three-quarter turn
        • majority of the affected ears lost around one-half turn of the cochlea
      • decreased outer hair cell stereocilia number (MGI Ref ID J:140027)
        • some of the outer hair cell bundles are missing in the middle and apical regions
      • increased cochlear inner hair cell number (MGI Ref ID J:140027)
        • there are slightly increased numbers of inner hair cells in the middle region of E18.5 embryos
        • in the apex region, cochleae had many extra hair cells (nearly two rows of inner hair cells)
    • abnormal semicircular canal morphology (MGI Ref ID J:140027)
      • abnormal semicircular canal ampulla morphology (MGI Ref ID J:140027)
        • 2 of 22 mice at E18.5 have truncated ampulla, one posteriorly and one anteriorly
      • decreased posterior semicircular canal size (MGI Ref ID J:140027)
        • 2 mice of 22 mice at E18.5 have truncated posterior semicircular canals
      • decreased superior semicircular canal size (MGI Ref ID J:140027)
        • 2 mice of 22 mice at E18.5 have truncated anterior semicircular canals
    • abnormal vestibular saccule morphology (MGI Ref ID J:140027)
      • 5 of 22 mice at E18.5 have malformed cochlea
    • decreased vestibular hair cell number (MGI Ref ID J:140027)
      • there is a slight but significant reduction in the number of hair cells found in the sacculle of mice (1508 vs. 1705 in wild-type)
    • short endolymphatic duct (MGI Ref ID J:140027)
      • 4 mice of 22 mice at E18.5 have a truncated endolymphatic duct
  • nervous system phenotype
    • abnormal orientation of cochlear hair cell stereociliary bundles (MGI Ref ID J:140027)
      • adult mice exhibit short and disorganized stereocilia bundles
    • decreased outer hair cell stereocilia number (MGI Ref ID J:140027)
      • some of the outer hair cell bundles are missing in the middle and apical regions
    • decreased vestibular hair cell number (MGI Ref ID J:140027)
      • there is a slight but significant reduction in the number of hair cells found in the sacculle of mice (1508 vs. 1705 in wild-type)
    • increased cochlear inner hair cell number (MGI Ref ID J:140027)
      • there are slightly increased numbers of inner hair cells in the middle region of E18.5 embryos
      • in the apex region, cochleae had many extra hair cells (nearly two rows of inner hair cells)
Eya1tm1Rilm/Eya1+
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • renal/urinary system phenotype
    • absent kidney (MGI Ref ID J:57313)
      • unilateral kidney agenesis was seen in 2 out of 11 mutants
  • hearing/vestibular/ear phenotype
    • decreased cochlear coiling (MGI Ref ID J:119574)
      • at E17.5, 17 of 20 heterozygous mutant inner ears (9 of 10 embryos) display a shortened cochlea with < 1.75 to ~1.5 turns, 3 of 20 (2 embryos) display < 1.5 to ~1.25 turns, and 1 innear ear (1 embryo) completes < 1.25 to ~1.0 turns; no less than 1.0 turns are observed
    • short endolymphatic duct (MGI Ref ID J:119574)
      • at E17.5, 2 of 20 heterozygous mutant inner ears (2 of 10 embryos) display a truncated endolymphatic duct/sac
Eya1tm1Rilm/Eya1tm1Rilm
        either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * BALB/c) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
  • mortality/aging
    • complete neonatal lethality (MGI Ref ID J:57313)
      • all homozygotes die at birth
  • craniofacial phenotype
    • abnormal cranium morphology (MGI Ref ID J:57313)
      • abnormal basisphenoid bone morphology (MGI Ref ID J:57313)
      • abnormal hyoid bone morphology (MGI Ref ID J:57313)
        • hyoid bone is malformed
        • abnormal hyoid bone lesser horn morphology (MGI Ref ID J:57313)
          • lesser horns are malformed
      • abnormal jaw morphology (MGI Ref ID J:57313)
        • absent maxilla (MGI Ref ID J:57313)
        • absent premaxilla (MGI Ref ID J:57313)
        • decreased maxillary shelf size (MGI Ref ID J:57313)
        • mandible hypoplasia (MGI Ref ID J:57313)
      • abnormal pterygoid process morphology (MGI Ref ID J:57313)
      • abnormal temporal bone morphology (MGI Ref ID J:119574)
        • at E12.5, the cartilage primordium of the temporal bone is severely affected
      • abnormal zygomatic bone morphology (MGI Ref ID J:57313)
      • decreased palatine shelf size (MGI Ref ID J:57313)
      • small cranium (MGI Ref ID J:57313)
    • abnormal middle ear ossicle morphology (MGI Ref ID J:57313)
      • abnormal incus morphology (MGI Ref ID J:57313)
        • the incus is present but malformed
      • absent malleus processus brevis (MGI Ref ID J:57313)
        • the short process of the malleus is usually absent
      • absent stapes (MGI Ref ID J:57313)
        • the stapes is usually absent
      • fusion of middle ear ossicles (MGI Ref ID J:57313)
        • middle ear ossicles are frequently fused
    • abnormal nasal septum morphology (MGI Ref ID J:57313)
      • enlarged
    • abnormal outer ear morphology (MGI Ref ID J:57313)
      • preauricular pits are seen
      • abnormal external auditory canal (MGI Ref ID J:57313)
        • atresia of the external auditory canal is seen
      • absent outer ear (MGI Ref ID J:57313)
        • the external auditory meati are absent or when present end blindly
        • anotia (MGI Ref ID J:57313)
          • the auricles are missing or malformed
    • abnormal palate development (MGI Ref ID J:57313)
      • on a C57BL/6 background abnormal fusion of the palatal shelves to the nasal septum is seen
    • cleft secondary palate (MGI Ref ID J:57313)
      • on mixed 129 or 129 and BALB/c backgrounds cleft secondary palate is seen
    • microcephaly (MGI Ref ID J:57313)
  • endocrine/exocrine gland phenotype
    • abnormal ultimobranchial body (MGI Ref ID J:79848)
      • unilateral or bilateral persistence of ultimobranchial bodies outside the thyroid gland is seen at E15.5
    • absent parathyroid glands (MGI Ref ID J:57313)
    • small thyroid gland (MGI Ref ID J:79848)
      • hypoplastic thyroid lobes with fewer calcitonin producing cells are seen
      • some mutants lack an isthmus in the thyroid gland
  • hearing/vestibular/ear phenotype
    • abnormal inner ear morphology (MGI Ref ID J:119574)
      • marker gene analyses at different stages indicate that primordia fated to form the endolymphatic duct are present but fail to outgrow normally
      • at E12.5, homozygotes display two vesicle-like structures, with the one located medially showing strong Foxi1 expression in all 6 embryos examined, indicating that it is the endolymphatic duct/sac
      • abnormal endolymphatic duct morphology (MGI Ref ID J:119574)
        • at E10.5 to E11.5, an abnormal vesicular structure is formed posteroventrally in 10 ears of 7 mutant embryos instead of a normal endolymphatic duct
        • marker gene analysis indicates that development of endolymphatic duct is initiated but fails to form a normal structure
        • absent endolymphatic duct (MGI Ref IDs J:57313, J:119574)
          • the endolymphatic duct is absent or malformed (MGI Ref ID J:57313)
          • at E10.5 to E11.5, a normal outgrowth of the endolymphatic duct is absent in all 20 ears of 10 mutant embryos studied; narrower dorsal tips are observed (MGI Ref ID J:119574)
          • paintfilling confirms absence of the outgrowth of endolymphatic duct in all 8 mutant ears analyzed (MGI Ref ID J:119574)
      • abnormal lateral semicircular canal morphology (MGI Ref ID J:119574)
      • abnormal posterior semicircular canal (MGI Ref ID J:119574)
      • absent cochlea (MGI Ref ID J:119574)
        • at E10.5 to E11.5, all homozygotes lack visible development of the cochlea
      • absent inner ear (MGI Ref ID J:57313)
      • absent vestibule (MGI Ref ID J:119574)
        • at E10.5 to E11.5, all homozygotes lack visible development of the vestibule
    • abnormal middle ear morphology (MGI Ref ID J:57313)
      • the tympanic cavity does not form
      • tympanic bulla is absent
      • abnormal middle ear ossicle morphology (MGI Ref ID J:57313)
        • abnormal incus morphology (MGI Ref ID J:57313)
          • the incus is present but malformed
        • absent malleus processus brevis (MGI Ref ID J:57313)
          • the short process of the malleus is usually absent
        • absent stapes (MGI Ref ID J:57313)
          • the stapes is usually absent
        • fusion of middle ear ossicles (MGI Ref ID J:57313)
          • middle ear ossicles are frequently fused
      • abnormal tympanic membrane morphology (MGI Ref ID J:57313)
        • the eardrums are malfo rmed
      • decreased tympanic ring size (MGI Ref ID J:57313)
    • abnormal otic vesicle development (MGI Ref IDs J:57313, J:119574)
      • (MGI Ref ID J:119574)
      • the otic vesicle from which inner ear structures arise fails to form, associated with increased apoptosis (MGI Ref ID J:57313)
    • abnormal outer ear morphology (MGI Ref ID J:57313)
      • preauricular pits are seen
      • abnormal external auditory canal (MGI Ref ID J:57313)
        • atresia of the external auditory canal is seen
      • absent outer ear (MGI Ref ID J:57313)
        • the external auditory meati are absent or when present end blindly
        • anotia (MGI Ref ID J:57313)
          • the auricles are missing or malformed
    • decreased otic epithelium proliferation (MGI Ref ID J:119574)
      • at E8.5, the number of BrdU-labeled cells in the mutant otic placode is ~80% of wild-type embryos
      • at E9.0 and E9.5, BrdU-positive cells are significantly reduced in the otic cup and vesicle, and are only 60% and 40% of wild-type embryos, respectively, in the otic placode
    • increased otic epithelium apoptosis (MGI Ref IDs J:57313, J:119574)
      • (MGI Ref ID J:57313)
      • increased cell death in the otic epithelium is first noted at ~E9.0, with numerous apoptotic bodies found in the rims of the otic cup, as shown by TUNEL analysis (MGI Ref ID J:119574)
      • at E9.5, increased cell death is apparent in the lateral wall of the otic vesicle, while a few apoptotic cells are also observed in the medial wall of the otic vesicle (MGI Ref ID J:119574)
      • by E10.5, apopt otic cells are found throughout the otic vesicle (MGI Ref ID J:119574)
    • small otic vesicle (MGI Ref ID J:119574)
      • at E11.5, homozygotes show a significant size reduction of the otocyst relative to wild-type mice
  • immune system phenotype
  • renal/urinary system phenotype
    • abnormal metanephric mesenchyme morphology (MGI Ref ID J:57313)
      • the metanephric mesenchyme undergoes apoptosis, disappearing by E12.5
    • absent kidney (MGI Ref ID J:57313)
      • bilateral kidney agenesis is seen in all homozygotes
    • absent ureter (MGI Ref ID J:57313)
      • ureters are absent in all homozygotes due to failure of ureteric bud outgrowth and metanephric induction
    • absent ureteric bud (MGI Ref ID J:57313)
      • the ureteric bud fails to form
  • skeleton phenotype
    • abnormal cranium morphology (MGI Ref ID J:57313)
      • abnormal basisphenoid bone morphology (MGI Ref ID J:57313)
      • abnormal hyoid bone morphology (MGI Ref ID J:57313)
        • hyoid bone is malformed
        • abnormal hyoid bone lesser horn morphology (MGI Ref ID J:57313)
          • lesser horns are malformed
      • abnormal jaw morphology (MGI Ref ID J:57313)
        • absent maxilla (MGI Ref ID J:57313)
        • absent premaxilla (MGI Ref ID J:57313)
        • decreased maxillary shelf size (MGI Ref ID J:57313)
        • mandible hypoplasia (MGI Ref ID J:57313)
      • abnormal pterygoid process morphology (MGI Ref ID J:57313)
      • abnormal temporal bone morphology (MGI Ref ID J:119574)
        • at E12.5, the cartilage primordium of the temporal bone is severely affected
      • abnormal zygomatic bone morphology (MGI Ref ID J:57313)
      • decreased palatine shelf size (MGI Ref ID J:57313)
      • small cranium (MGI Ref ID J:57313)
    • abnormal ischium morphology (MGI Ref ID J:57313)
      • the ischium and pubis are fused
    • abnormal middle ear ossicle morphology (MGI Ref ID J:57313)
      • abnormal incus morphology (MGI Ref ID J:57313)
        • the incus is present but malformed
      • absent malleus processus brevis (MGI Ref ID J:57313)
        • the short process of the malleus is usually absent
      • absent stapes (MGI Ref ID J:57313)
        • the stapes is usually absent
      • fusion of middle ear ossicles (MGI Ref ID J:57313)
        • middle ear ossicles are frequently fused
    • abnormal pubis morphology (MGI Ref ID J:57313)
      • the ischium and pubis are fused
    • abnormal rib-sternum attachment (MGI Ref ID J:57313)
      • T7 ribs do not fuse with the sternum
    • abnormal thyroid cartilage morphology (MGI Ref ID J:57313)
      • lateral processes of the thyroid cartilage, which normally connect with the cricoid cartilage, are absent or malformed
    • cervical vertebral fusion (MGI Ref ID J:57313)
      • the mutant atlas and axis are fused
    • rib fusion (MGI Ref ID J:57313)
      • ribs are fused bilaterally
  • vision/eye phenotype
    • eyelids open at birth (MGI Ref ID J:57313)
  • nervous system phenotype
    • abnormal geniculate ganglion morphology (MGI Ref ID J:57313)
      • the geniculate ganglion is absent
    • absent petrosal ganglion (MGI Ref ID J:57313)
    • absent vestibular ganglion (MGI Ref ID J:57313)
  • embryogenesis phenotype
    • abnormal branchial pouch morphology (MGI Ref IDs J:79848, J:57313)
      • increased apoptosis is seen in the surface ectoderm of the 3rd and 4th pouches (MGI Ref ID J:79848)
      • a persistent cleft of the first pharyngeal pouch is seen at E10.5 (MGI Ref ID J:57313)
      • abnormal ultimobranchial body (MGI Ref ID J:79848)
        • unilateral or bilateral persistence of ultimobranchial bodies outside the thyroid gland is seen at E15.5
  • hematopoietic system phenotype
  • digestive/alimentary phenotype
    • abnormal palate development (MGI Ref ID J:57313)
      • on a C57BL/6 background abnormal fusion of the palatal shelves to the nasal septum is seen
    • cleft secondary palate (MGI Ref ID J:57313)
      • on mixed 129 or 129 and BALB/c backgrounds cleft secondary palate is seen
    • decreased maxillary shelf size (MGI Ref ID J:57313)
    • decreased palatine shelf size (MGI Ref ID J:57313)
  • respiratory system phenotype
    • abnormal nasal septum morphology (MGI Ref ID J:57313)
      • enlarged
    • abnormal thyroid cartilage morphology (MGI Ref ID J:57313)
      • lateral processes of the thyroid cartilage, which normally connect with the cricoid cartilage, are absent or malformed
Eya1tm1Rilm/Eya1tm1Rilm
        involves: 129 * C3HeB/FeJ
  • hearing/vestibular/ear phenotype
    • absent inner ear (MGI Ref ID J:140027)
      • no inner ear structures form in these mice
Eya1tm1Rilm/Eya1tm1Rilm
        involves: 129S1/Sv * 129X1/SvJ
  • cellular phenotype
    • abnormal cell differentiation (MGI Ref ID J:171504)
      • loss of distal lung epithelial progenitors from E14 - E14.5
    • abnormal mitotic spindle (MGI Ref ID J:171504)
      • the orientation of the mitotic spindle in distal lung epithelium cells is mostly parallel to the basement membrane as opposed to perpendicular in controls
  • respiratory system phenotype
    • abnormal lung epithelium morphology (MGI Ref ID J:171504)
      • loss of distal lung epithelial progenitors from E14 - E14.5
      • reduced epithelial branching
      • increased epithelial differentiation
    • small lung (MGI Ref ID J:171504)
      • reduced lung size

Strain of Origin: B6; 129

Strain genetic background: C57BL/6 (N10+)

Strain Development: see alteration

Suggested Control Mice: Wild-type littermates

Research Applications

    Strain Origin

    Donor: Richard Maas, Ph.D., Brigham and Women's Hospital / Harvard Med School

    Primary Reference:

    Xu PX, Adams J, Peters H, Brown MC, Heaney S, Maas R. Eya1-deficient mice lack ears and kidneys and show abnormal apoptosis of organ primordia. Nat Genet. 1999 Sep;23(1):113-7. (Medline PMID: 10471511 )

    Colony and Husbandry Information

    Special Considerations

    None

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Appearance

    Coat color: Black

    Other:

    Breeding

    MMRRC Breeding System: Inbred

    Breeding Scheme(s):

    • Heterozygous female x Wildtype male

    Generation: Unknown

    Overall Breeding Performance: Normal when bred Heterozygous x Wildtype.

    Reproductive Statistics

    Viability and Fertility:FemaleMale
    Homozygotes are viable: No No
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
     
    Age Reproductive Decline: 16 weeks 16 weeks

    Average litter size: 6 - 8

    Recommended wean age: 3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    000361-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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