Strain Name    : B6.129-Eya1tm1Rilm/Mmucd

Stock Number : 000361-UCD

Other Names   : Eya1 knockout, B6.129-Eya1tm1Rilm/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Eya1tm1Rilm
Name: targeted mutation 1, Richard Maas
Chromosome: 1
Alteration at locus: Knockout
Gene Symbol: Eya1
Name: eyes absent 1 homolog (Drosophila)
Chromosome: 1
Alteration at locus: Knockout
Genetic Alterations:
Isolated Eya1 genomic clones encoding the Eya domain from a 129/SvJ BAC genomic library. Correct targeting resulted in deletion from 305 bp upstream of exon 10 to 1,068 bp downstream of exon 13 in the Eya domain region and replacement with pgk-neo. The targeting construct was electroporated into R1 ES cells.

Genotype Determination:

ES Cell Line: R1 derived from 129

Phenotype

Eya1-/- mice die perinatally and display a range of anomalies, including cleft palate, reduced head size and a variety of skeletal abnormalities, including in the skull. The cleft palate phenotype differs with background. Eya1-/- in the 129/SV or Balb/C background results in secondary cleft palate whereas Eya1-/- in the C57BL/6J results in a fusion of the palatal shelves with the nasal septum. Eya1 null mice also display renal, otic, thymus, and parathyroid agenesis.

Mammalian Phenotype Terms:
Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * BALB/c) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129 * C3HeB/FeJ )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129 * C57BL/6 * CD-1 * SJL )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

Allelic Composition: Eya1tm1Rilm/Eya1+ (Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * BALB/c) )

Allelic Composition: Eya1tm1Rilm/Eya1+ (Genetic Background: involves: 129 * C3HeB/FeJ )

Allelic Composition: Eya1tm1Rilm/Eya1+ (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J )

Allelic Composition: Eya1bor/Eya1tm1Rilm (Genetic Background: involves: 129 * C3HeB/FeJ )

Strain of Origin: B6; 129

Strain genetic background: C57BL/6 (N10+)

Strain Development: see alteration

Suggested Control Mice: Wild-type littermates

Donor: Richard Maas, Ph.D., Brigham and Women's Hospital / Harvard Med School

Primary Reference:

Xu PX, Adams J, Peters H, Brown MC, Heaney S, Maas R. Eya1-deficient mice lack ears and kidneys and show abnormal apoptosis of organ primordia. Nat Genet. 1999 Sep;23(1):113-7. (Medline PMID: 10471511 )

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:

MMRRC Breeding System: Inbred

Breeding Scheme(s):

  • Heterozygous female x Wildtype male

Generation: Unknown

Overall Breeding Performance: Normal when bred Heterozygous x Wildtype.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 16 weeks 16 weeks

Average litter size: 6 - 8

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000361-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.