Strain Name:
B6.129-Eya1tm1Rilm/Mmucd
Stock Number:
000361-UCD
Citation ID:
RRID:MMRRC_000361-UCD
Other Names:
Eya1 knockout, B6.129-Eya1tm1Rilm/Mmcd

Strain Information

Eya1tm1Rilm
Name: EYA transcriptional coactivator and phosphatase 1; targeted mutation 1, Richard Maas
Synonyms: Eya1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Genetic Alterations
Isolated Eya1 genomic clones encoding the Eya domain from a 129/SvJ BAC genomic library. Correct targeting resulted in deletion from 305 bp upstream of exon 10 to 1,068 bp downstream of exon 13 in the Eya domain region and replacement with pgk-neo. The targeting construct was electroporated into R1 ES cells.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Eya1-/- mice die perinatally and display a range of anomalies, including cleft palate, reduced head size and a variety of skeletal abnormalities, including in the skull. The cleft palate phenotype differs with background. Eya1-/- in the 129/SV or Balb/C background results in secondary cleft palate whereas Eya1-/- in the C57BL/6J results in a fusion of the palatal shelves with the nasal septum. Eya1 null mice also display renal, otic, thymus, and parathyroid agenesis.
Mammalian Phenotype Terms
Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * BALB/c) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129 * C3HeB/FeJ )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129 * C57BL/6 * CD-1 * SJL )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

Allelic Composition: Eya1tm1Rilm/Eya1tm1Rilm (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

Allelic Composition: Eya1tm1Rilm/Eya1+ (Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * BALB/c) )

Allelic Composition: Eya1tm1Rilm/Eya1+ (Genetic Background: involves: 129 * C3HeB/FeJ )

Allelic Composition: Eya1tm1Rilm/Eya1+ (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J )

Allelic Composition: Eya1bor/Eya1tm1Rilm (Genetic Background: involves: 129 * C3HeB/FeJ )

MeSH Terms
  • Animals
  • Apoptosis
  • Bone and Bones/abnormalities
  • Bone and Bones/anatomy & histology
  • Bone and Bones/embryology
  • Branchio-Oto-Renal Syndrome/genetics
  • DNA-Binding Proteins/metabolism
  • Ear/abnormalities
  • Ear/anatomy & histology
  • Ear/embryology
  • Evoked Potentials, Auditory/physiology
  • Fibroblast Growth Factor 3
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Glial Cell Line-Derived Neurotrophic Factor
  • Hearing Loss, Conductive/metabolism
  • Homeodomain Proteins/metabolism
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Kidney/abnormalities
  • Kidney/anatomy & histology
  • Kidney/embryology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Nerve Growth Factors
  • Nerve Tissue Proteins/metabolism
  • Nuclear Proteins
  • PAX2 Transcription Factor
  • PAX8 Transcription Factor
  • Paired Box Transcription Factors
  • Protein Tyrosine Phosphatases
  • Proto-Oncogene Proteins/metabolism
  • Signal Transduction
  • Time Factors
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Trans-Activators/physiology
  • Transcription Factors/metabolism
Strain Development
see alteration
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
Donor
Richard Maas, Ph.D., Brigham and Women's Hospital / Harvard Medical School
Primary Reference
Xu PX, Adams J, Peters H, Brown MC, Heaney S, Maas R. Eya1-deficient mice lack ears and kidneys and show abnormal apoptosis of organ primordia. Nat Genet. 1999 Sep;23(1):113-7. (Medline PMID: 10471511)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Inbred
Breeding Scheme(s)
  • Heterozygous female x Wildtype male
Generation
Unknown
Overall Breeding Performance
Normal when bred Heterozygous x Wildtype.
Average litter size
6 - 8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000361-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.