Strain Name    : B6.129S1(Cg)-Mrgprdtm1.1(cre)And/Mmnc

Stock Number : 036118-UNC

Gene Information

(provided by MGI)
Target: Mrgprdtm1.1(cre)And
Name: targeted mutation 1.1, David Anderson
Chromosome: 7
Alteration at locus: Knock-In
Target: Mrgprd
Name: MAS-related GPR, member D
Chromosome: 7
Alteration at locus: Knock-In
Genetic Alterations:
Most of the open reading frame was replaced with a cassette containing EGFP, cre, and frt flanked neo that was inserted out of frame with the initiation site. The inserted cassette replaced amino acids 20 through 315 of the total 321 amino acids of the endogenous protein. The frt-flanked cassette is removed by mating to FLPe deleter mice.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line:

    Phenotype

    Homozygous mice have significantly diminished sensitivity to cold, heat, and mechanical stimuli in a population of nonpeptidergic neurons. Heterozygouse mice are the same as homozygous, with a lower rheobase, and a relatively higher firing rate following the application of Mrgprd ligands than -/- animals.

    Mammalian Phenotype Terms:
    Allelic Composition: Mrgprdtm1.1(cre)And/Mrgprdtm1.1(cre)And (Genetic Background: B6.129S1-Mrgprdtm1.1(cre)And )

    Strain of Origin: B6;129S1

    Strain genetic background: B6.129S1

    Strain Development: Chimeric mice were produced by blastocyst injection and were mated to human β-actin FLPe deleter mice to remove the frt-flanked selection cassette and then were mated to C57BL/6 mice to establish the line. All knock-in lines used for this study were backcrossed to C57BL/6 mice for five or more generations.

    Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC StrainReference StrainsGenomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129S1-Mrgprdtm1.1(cre)And/Mmnc C57BL/6J 129S1/SvImJ 2558 23 0 0


    Suggested Control Mice: Wild-type littermates

    Donor: Mark Zylka, Ph.D., University of North Carolina at Chapel Hill School of Medicine.

    Primary Reference:

    Rau KK, McIlwrath SL, Wang H, Lawson JJ, Jankowski MP, Zylka MJ, Anderson DJ, Koerber HR. Mrgprd Enhances Excitability in Specific Populations of Cutaneous Murine Polymodal Nociceptor. J Neurosci. 2009 July 1;29(26):8612–8619. (Medline PMID: 19571152).

     

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Black

    Other:

    MMRRC Breeding System: Random intra-strain mating

    Breeding Scheme(s):

    • Homozygous female x Homozygous male

    Generation: N5

    Overall Breeding Performance: Excellent

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Average litter size: Unknown

    Recommended wean age: Unknown

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    036118-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    036118-UNC-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.