Name: targeted mutation 1.1, David Anderson
Alteration at locus: Knock-In
Name: MAS-related GPR, member D
Alteration at locus: Knock-In
ES Cell Line: CJ7
Homozygous mice have significantly diminished sensitivity to cold, heat, and mechanical stimuli in a population of nonpeptidergic neurons. Heterozygouse mice are the same as homozygous, with a lower rheobase, and a relatively higher firing rate following the application of Mrgprd ligands than -/- animals.
Strain of Origin: B6;129S1
Strain genetic background: B6.129S1
Strain Development: Chimeric mice were produced by blastocyst injection and were mated to human β-actin FLPe deleter mice to remove the frt-flanked selection cassette and then were mated to C57BL/6 mice to establish the line. All knock-in lines used for this study were backcrossed to C57BL/6 mice for five or more generations.
Strain Genetic Background Validation:
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
|MMRRC Strain||Reference Strains||Genomic makeup (Mb)|
|Strain Name||MMRRC ID #||Reference 1||Reference 2||Ref 1||Ref 2||Het||Unknown|
Suggested Control Mice: Wild-type littermates
Donor: Mark Zylka, Ph.D., University of North Carolina at Chapel Hill School of Medicine.
Rau KK, McIlwrath SL, Wang H, Lawson JJ, Jankowski MP, Zylka MJ, Anderson DJ, Koerber HR. Mrgprd Enhances Excitability in Specific Populations of Cutaneous Murine Polymodal Nociceptor. J Neurosci. 2009 July 1;29(26):8612–8619. (Medline PMID: 19571152).
Coat color: Black
MMRRC Breeding System: Random intra-strain mating
Overall Breeding Performance: Excellent
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: Unknown
Recommended wean age: Unknown
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.