Strain Name:
B6.Cg-Mapttm1Hnd Tg(MAPT*)1Gds/Mmjax
Stock Number:
036171-JAX
Citation ID:
RRID:MMRRC_036171-JAX

Gene Information

Mapttm1Hnd
Name: microtubule-associated protein tau; targeted mutation 1, Hana N Dawson
Synonyms: TAU-, MaptKO(Duke)
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
Mapt
Name: microtubule-associated protein tau
Synonyms: Mtapt, Tau
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
NCBI: 17762
HGNC: HGNC:6893
Homologene: 74962
Tg(MAPT*)1Gds
Name: transgene insertion 1, Gerard Schellenberg
Synonyms: hT-PAC-E10+14
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The transgenic construct contains the 201-kb P1 artificial chromosome (PAC) 61D6 and includes 5 kb of sequence upstream of the human MAPT promoter and an additional 62 kb downstream of the 3' end of MAPT. The downstream segment also contains the 3' end of the gene KIAA1267, however, human MAPT is the only complete gene in the PAC. The E10+14 mutation (PMID:10318930) results in the T-to-C exchange in intron 10 (NM_001123066.3:c.1920+14T>C). The mutation alters the alternative splicing of MAPT transcripts resulting in an increased 4R/3R tau ratio.

The endogenous, murine Mapt gene has been knocked out.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Mice that are homozygous for the targeted mutation and hemizygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These double-mutant mice produce mutated human tau (MAPT), but do not produce mouse tau (Mapt). The hT-PAC-E10+14 mouse model (with the E10+14 MAPT mutation) expresses all six human tau isoforms in the mouse brain with a [3-repeat (3R)-tau to 4-repeat (4R)-tau] isoform ratio of approximately 1:4, rather than the expected 1:1. The E10+14 mutation in humans increases exon 10 splicing and is associated with frontotemporal dementia with parkinsonism-linked to chromosome 17 (FTDP-17). No gross or microscopic pathologic lesions were observed in these mice. This mutant mouse strain may be useful in studies of tau isoforms in neurodegenerative disease. Heterozygous mice were not evaluated.
Mammalian Phenotype Terms
Allelic Composition: Mapttm1Hnd/Mapttm1Hnd (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

Allelic Composition: Mapttm1Hnd/Mapt+ (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Aging/metabolism
  • Animals
  • Animals, Newborn
  • Brain/cytology
  • Brain/growth & development
  • Brain/metabolism
  • Exons/genetics
  • Frontal Lobe/cytology
  • Frontal Lobe/growth & development
  • Frontal Lobe/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Hippocampus/cytology
  • Hippocampus/growth & development
  • Hippocampus/metabolism
  • Humans
  • Mice
  • Mice, Transgenic
  • Neurons/metabolism
  • Phosphorylation
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Species Specificity
  • Synaptic Membranes/metabolism
  • Synaptic Membranes/ultrastructure
  • tau Proteins/genetics
  • tau Proteins/metabolism
  • Aged
  • Alternative Splicing
  • Brain/pathology
  • Chromosomes, Human, Pair 17
  • Dementia/genetics
  • Female
  • Histocytochemistry
  • Male
  • Microtubules/pathology
  • Middle Aged
  • Mutation
  • Parkinson Disease/genetics
  • Pedigree
  • Protein Binding
  • RNA, Messenger/metabolism
  • Regulatory Sequences, Nucleic Acid/genetics
Strain Development
This transgene was microinjected into fertilized (C57BL/6 x C3H)F1 hybrid oocytes and founder mice were backcrossed to C57BL/6J mice for approximately 10+ generations. This transgenic line was crossed to B6.129X1-Mapttm1Hnd/J mice (JAX Strain #007251) to generate mice that were homozygous for the targeted mutation and hemizygous for the transgene. The colony has been maintained by sibling mating for three generations prior to donation to the MMRRC.
Suggested Control Mice
Wild-type littermates
  • Models for Human Disease
    • Alzheimer's disease
    • frontotemporal dementia
  • Neurobiology
Donor
Gerard Schellenberg, Ph.D., Perelman School of Medicine at the University of Pennsylvania.
Hana N. Dawson, Ph.D., Duke University.
Primary Reference

McMillan P, Korvatska E, Poorkaj P, Evstafjeva Z, Robinson L, Greenup L, Leverenz J, Schellenberg GD, D'Souza I. Tau isoform regulation is region- and cell-specific in mouse brain. J Comp Neurol. 2008 Dec 20;511(6):788-803. (Medline PMID: 18925637)

D'Souza I, Poorkaj P, Hong M, Nochlin D, Lee VM, Bird TD, Schellenberg GD. Missense and silent tau gene mutations cause frontotemporal dementia with parkinsonism-chromosome 17 type, by affecting multiple alternative RNA splicing regulatory elements. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5598-603. (Medline PMID: 10318930)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Breeding Scheme(s)

Sib-Mating

  • While maintaining a live colony, these mice are bred as homozygous for the targeted mutation and hemizygous for the transgene.
  • Homozygous KO, Hemizygous Tg female x Homozygous KO, Wild-type Tg male sibling or reciprocol mating
Generation
N10+ (C57BL/6J), F3
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Bred to Homozygosity
Yes
Average litter size
5
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036171-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036171-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.