Strain Name:
B6.129X1-Ptpn11tm3Bgn/Mmucd
Stock Number:
036194-UCD
Citation ID:
RRID:MMRRC_036194-UCD
Other Names:
Shp2 floxed, B6.129X1-Ptpn11tm3Bgn/Mmcd

Strain Information

Ptpn11tm3Bgn
Name: protein tyrosine phosphatase, non-receptor type 11; targeted mutation 3, Benjamin G Neel
Synonyms: Shp2fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Ptpn11
Name: protein tyrosine phosphatase, non-receptor type 11
Synonyms: Shp2, SHP-2, SH-PTP2, PTP1D, PTP2C, 2700084A17Rik, SH2 domain-containing protein tyrosine phosphatase-2, Syp
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Conditional
NCBI: 19247
HGNC: HGNC:9644
Homologene: 2122
Genetic Alterations
Two loxP sites were inserted flanking Ptpn11 exon11.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Shp2 is required for full activation of the Ras/Erk signaling downstream of many receptor tyrosine kinases and cytokine receptors. It is implicated in survival, proliferation and differentiation of many cell type. Tissue-specific phenotype are produced when these flox mice are crossed to different cre. For example, Shp2 is required for survival and maintenance of hematopoietic stem cell and immature hematepoietic progenitors (see Chan et al, Blood 2011 117(16):4253-61. 2011). Germline Shp2 mutations underlie ~50% of Noonan syndrome (NS), which is characterized by short stature, skeletal abnormalities, cardiac defects, learning disabilities, and a predisposition to hematologic abnormalities, particularly juvenile myelomonocytic leukemia (JMML). Somatic gain-of-function mutations in Shp2 also are the most common cause of sporadic JMML
MeSH Terms
  • Animals
  • Bone Marrow/pathology
  • Cell Cycle
  • Cell Death
  • Epistasis, Genetic
  • Gene Deletion
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • Mice
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/metabolism
  • Proto-Oncogene Proteins p21(ras)/genetics
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Cardiomegaly/enzymology
  • Cardiomegaly/genetics
  • Cardiomegaly/physiopathology
  • Cardiomyopathy, Dilated/enzymology
  • Cardiomyopathy, Dilated/genetics
  • Cardiomyopathy, Dilated/physiopathology
  • LEOPARD Syndrome/enzymology
  • LEOPARD Syndrome/genetics
  • MAP Kinase Signaling System/physiology
  • Male
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitogen-Activated Protein Kinase 1/antagonists & inhibitors
  • Mitogen-Activated Protein Kinase 1/physiology
  • Mitogen-Activated Protein Kinase 3/antagonists & inhibitors
  • Mitogen-Activated Protein Kinase 3/physiology
  • Models, Animal
  • Myocytes, Cardiac/enzymology
  • Noonan Syndrome/enzymology
  • Noonan Syndrome/genetics
  • Organ Specificity
  • Phenotype
  • Pressure
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/deficiency
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/physiology
  • Signal Transduction/physiology
  • rho GTP-Binding Proteins/antagonists & inhibitors
  • rho GTP-Binding Proteins/physiology
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Models for Human Disease
    • Noonan syndrome (NS)
    • juvenile myelomonocytic leukemia (JMML)
Donor
Benjamin G. Neel, M.D., Ontario Cancer Institute.
Primary Reference
Chan G, Cheung LS, Yang W, Milyavsky M, Sanders AD, Gu S, Hong WX, Liu AX, Wang X, Barbara M, Sharma T, Gavin J, Kutok JL, Iscove NN, Shannon KM, Dick JE, Neel BG, Braun BS. Essential role for Ptpn11 in survival of hematopoietic stem and progenitor cells. Blood. 2011 Apr 21;117(16):4253-61. Epub 2011 Feb 25. (Medline PMID: 21398220).

Kontaridis MI, Yang W, Bence KK, Cullen D, Wang B, Bodyak N, Ke Q, Hinek A, Kang PM, Liao R, Neel BG. Deletion of Ptpn11 (Shp2) in cardiomyocytes causes dilated cardiomyopathy via effects on the extracellular signal-regulated kinase/mitogen-activated protein kinase and RhoA signaling pathways. Circulation. 2008 Mar 18;117(11):1423-35. Epub 2008 Mar 3. (Medline PMID: 18316486).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

C57BL/6 female x Heterozygous male or reciprocol

Homozygous female x Heterozygous male sibling or reciprocol

Heterozygous female x Heterozygous male sibling

Generation
N12
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036194-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036194-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036194-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.