Strain Name:
STOCK Dnah1tm1Ihgg/Mmmh
Stock Number:
036195-MU
Citation ID:
RRID:MMRRC_036195-MU

Strain Information

Dnah1tm1Ihgg
Name: dynein, axonemal, heavy chain 1; targeted mutation 1, Institut fur Humangenetik
Synonyms: MDHC7-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: E030034C22Rik, Dnahc1, G1-415-19, MDHC7, ferf1, B230373P09Rik
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knockout
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Genetic Alterations
Exons 2-5 were replaced by the neo cassette.
Genotype Determination
ES Cell Line
R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl+)
Phenotype
Homozygous: males are infertile due to reduced sperm motility.

Heterozygous: mice are normal.
Mammalian Phenotype Terms
Allelic Composition: Dnah1tm1Ihgg/Dnah1tm1Ihgg (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CD-1 )

MeSH Terms
  • Animals
  • Base Sequence
  • Blotting, Western
  • Ciliary Motility Disorders/etiology
  • Ciliary Motility Disorders/metabolism
  • DNA Primers/chemistry
  • DNA Probes/chemistry
  • Dyneins/genetics
  • Dyneins/metabolism
  • Female
  • Gene Deletion
  • Gene Targeting
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Infertility, Male/etiology
  • Infertility, Male/metabolism
  • Male
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Oligospermia/etiology
  • Oligospermia/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spermatozoa/physiology
Strain Development
R1 ES cells were transfected with targeting vector. The chimera was bred with CD1 females to establish the dynenin-dirupted allele on CD1x129/Sv hybrid background. Next, heterozygous males and females were intercrossed.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Wolfgang Enegel, M.D., Georg-August-University of Goettingen.
Primary Reference
Neesen J, Kirschner R, Ochs M, Schmiedl A, Habermann B, Mueller C, Holstein AF, Nuesslein T, Adham I, Engel W. Disruption of an inner arm dynein heavy chain gene results in asthenozoospermia and reduced ciliary beat frequency. Hum Mol Genet. 2001 May 15;10(11):1117-28. (Medline PMID: 11371505).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Heterozygous female x Heterozygous male

Wild-type female x Heterozygous male

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036195-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
036195-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.