Strain Detail Sheet

Strain Name    :

B6.Cg-Mapttm1Hnd Tg(MAPT*V337M)1Gds/Mmjax

Stock Number :

036211-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mapttm1Hnd
Name: targeted mutation 1, Hana N Dawson
Alteration at locus: Knockout
Gene Symbol: Mapt
Name: microtubule-associated protein tau
Chromosome: 11
Alteration at locus: Knockout
Transgene: Tg(MAPT*V337M)1Gds (Human)
Name: transgene insertion 1 , Gerard Schellenberg
Alteration at locus: Transgenic

Genetic Alterations:
The transgenic construct contains the 201 kb P1 artificial chromosome (PAC) 61D6 and includes 5 kb of sequence upstream of the human MAPT promoter and an additional 62 kb downstream of the 3' end of MAPT. The downstream segment also contains the 3' end of the gene KIAA1267, however, human MAPT is the only complete gene in the PAC.   The V337M mutation, results in a change from GTG to ATG in exon 12, resulting in an amino acid substitution of methionine for valine at position 337.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Mice that are homozygous for the targeted mutation and hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These double mutant mice produce mutated human tau (MAPT), but do not produce mouse tau (Mapt).  The hT-PAC-337M mouse model (with the V337M MAPT mutation) expresses all 6 human tau isoforms in the mouse brain with a 3 repeat (3R)-tau to 4 repeat (4R)-tau isoform ratio of approximately 80% 3R-tau to 20% 4R-tau, rather than the expected human ratio of 1:1. The V337M mutation is associated with frontotemporal dementia with parkinsonism- linked to chromosome 17 (FTDP-17). No gross or microscopic pathologic lesions were observed in these mice. This mutant mouse strain may be useful in studies of tau isoforms in neurodegenerative disease.

Heterozygous mice have not been evaluated.

Strain of Origin: C57BL6/C3H/129X1

Strain genetic background: B6.Cg

Strain Development: This transgene was microinjected into fertilized C57BL/6 x C3H hybrid oocytes and founder mice were backcrossed to C57BL/6 mice for approximately 15 generations. This transgenic line was crossed to B6.129-Mapttm1Hnd mice to generate mice that were homozygous for the targeted mutation and hemizygous for the transgene.  The colony has been maintained by sibling mating.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Models for Human Disease
    • Alzheimer's disease
    • frontotemporal dementia
  • Neurobiology

Strain Origin

Donor: Gerard Schellenberg, Ph.D., Perelman School of Medicine at the University of Pennsylvania

Primary Reference:

McMillan P; Korvatska E; Poorkaj P; Evstafjeva Z; Robinson L; Greenup L; Leverenz J; Schellenberg GD; D'Souza I, Tau isoform regulation is region- and cell-specific in mouse brain. J Comp Neurol 2008 Dec 20;511(6):788-803 (Medline PMID: 18925637)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Sib-mating. While maintaining a live colony, these mice are bred as homozygous for the targeted mutation and hemizygous for the transgene.

Breeding Scheme(s):

  • Homozygous KO, Hemizygous Tg female x  Homozygous KO, Wild-type Tg male sibling or reciprocol mating

Generation: F3

Overall Breeding Performance: Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:FemaleMale
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes

Age Reproductive Decline: Unknown Unknown

Average litter size: 5

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
036211-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
036211-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/25/2014