Strain Name:
FVB/N-Hsp90aa1Tg(Tyr)2396BOve/Mmjax
Stock Number:
036273-JAX
Citation ID:
RRID:MMRRC_036273-JAX

Gene Information

Hsp90aa1Tg(Tyr)2396BOve
Name: transgene lentiviral insertion 2396B, Paul A Overbeek
Synonyms: OVE2396B, OVE#2396B
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Insertion
Genetic Alterations
These OVE2396B (OVE#2396B) mice harbor a mutation created by random insertion of the Tyro-IRES-sd-loxP-FUGW lentiviral transgene (LV2187). Using inverse PCR analysis, the lentiviral integration site was identified in exon 9 of the heat shock protein 90, alpha, class A member 1 gene (Hsp90aa1) on chromosome 12. The 3'-LTR is linked to the (+) strand of DNA at position 111,930,859 bp [NCB137/mm9; 3'-111,930,859(+)]. The orientation of the inserted lentivirus relative to the disrupted mouse gene was not determined.
Phenotype
The donating investigator reports the phenotype of homozygous mice as: males are sterile with small testes.
Strain Development
A lentiviral transgenic approach was used to generate these mice. The Tyro-sd-IRES-loxP-FUGW lentiviral transgene (LV2187) was designed with a mouse tyrosinase minigene (Tyro) followed by a splice-donor::IRES::loxP sequence in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro-sd-IRES-loxP replaced the ubiquitin-c promoter, EGFP, and WPRE sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (620 bp), promoter region (500 bp), and 1.7 kbp cDNA sequence (including the stop codon); all in antisense orientation relative to the FUGW backbone. The Tyro minigene is followed by an artificial splice donor sequence, an internal ribosome entry site (IRES), and a loxP site in this construct. This packaged lentiviral construct was injected subzonally (under the zona pellucida) into 1-8 cell stage FVB/N mouse embryos. Because of the random and multiple lentiviral integration sites of a transgene containing tyrosinase, founder mice (F0) may be identified by mosaic pigmentation.  Founder mice from line OVE#2396B were bred with FVB/N mice, and pigmented offspring (F1) were bred to FVB/N mice. The resulting offspring (F2) with identical coat colors (light grey) were subsequently inbred. Using inverse PCR analysis, the transgene integration site was identified in exon 9 of the heat shock protein 90, alpha, class A member 1 gene (Hsp90aa1) on chromosome 12 (specifically at the 3'-111,930,859(+) bp position). Transgenic males were sent to MMRRC. Upon arrival, these mice were bred to FVB/NJ inbred mice  for at least one generation to establish the live colony.
Suggested Control Mice
Wild-type littermates or FVB/NJ
  • chaperone, binds Unc45b
Donor
Paul A. Overbeek, Ph.D., Baylor College of Medicine

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Light grey
MMRRC Breeding System
Backcross or Sibmating
Breeding Scheme(s)
  • Heterozygous female x Heterozygouns male
  • Heterozygous female x male wild-type from colony male or recipricol mating
  • Heterozygous female x FVB/NJ male or recipricol mating
Overall Breeding Performance
Unknown
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Unknown
Recommended wean age
Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036273-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036273-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.