Strain Name:
B6;129S6-Gt(ROSA)26Sortm2Mgn/Mmucd
Stock Number:
036286-UCD
Citation ID:
RRID:MMRRC_036286-UCD
Other Names:
ROSA26{tm2Mgn}, Rosa26-Cherry, Rosa26.EN.Cherry.Neo

Gene Information

Reporter: Gt(ROSA)26Sortm2Mgn (Mus musculus (mouse))
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Mark A Magnuson
Synonyms: Gt(Rosa)26Sor, ROSA26
Chromosome: 6
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:5085307
Gene Symbol: Gt(ROSA)26Sor (Mus musculus (mouse))
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: Gtrgeo26, beta geo, Thumpd3as1, R26, Gtrosa26, ROSA26
Chromosome: 6
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:104735
Genetic Alterations
The coding sequences for a red (mCherry) flourescent protein gene were inserted into the ROSA26 gene locus in mESCs using a previously targeted mESC line containing a loxed cassette acceptor allele and the method of recombinase-mediated cassette exchange (RMCE).
Genotype Determination
Phenotype

Homozygous: No observed phenotype.

Heterozygous: This mouse line expresses mCherry, a red flourescent protein, under control of the endogenous ROSA26 gene locus.

Mammalian Phenotype Terms
Allelic Composition: Gt(ROSA)26Sortm2Mgn/Gt(ROSA)26Sor+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

MeSH Terms
  • Alleles
  • Animals
  • Chromosomes, Artificial, Bacterial/genetics
  • Embryo, Mammalian/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Fluorescence
  • Genetic Loci/genetics
  • Genetic Vectors/genetics
  • Luminescent Proteins/metabolism
  • Mice
  • Mutagenesis, Insertional/methods
  • Organ Specificity
  • Proteins/genetics
  • RNA, Untranslated
  • Rabbits
  • Recombinases/metabolism
Strain Development
This strain is currently in a mixed genetic background of both 129S6 and C57Bl/6J. Four backcrosses into C57Bl/6J have been performed.
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
  • Sensorineural
  • Virology
Donor
Mark Magnuson, Vanderbilt University.
Primary Reference

Chen SX, Osipovich AB, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. Quantification of factors influencing flourscent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech 2011 Jul; 4(4);537-47. Epub 2011 Feb 14. (Medline PMID: 21324933).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Other
Red fluorescence is visible in neonates.
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6J female x Heterozygous male or recipracol mating
Generation
N4
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036286-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
036286-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
036286-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.