Strain Name    : 129S6.Cg-Slc11a2mk/MdfMmnc

Stock Number : 036294-UNC

Gene Information

(provided by MGI)
Allele Symbol: Slc11a2mk
Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2; microcytic anemia
Chromosome: 15
Alteration at locus: Spontaneous Mutation
Gene Symbol: Slc11a2
Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Chromosome: 15
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
A G-to-A transition at a CpG dinucleotide, resulting in sub­stitution of arginine for glycine at codon 185 (Arg185). 

Genotype Determination:

ES Cell Line: Not Applicable

Phenotype

Homozygous phenotype:  Microcytic, hypochromic anemia. Homozygous mutants have decreased viability, and few survive to weaning.

Heterozygous phenotype: wild-type. 

Strain of Origin: MK/ReJ

Strain genetic background: 129S6/SvEvTac

Strain Development: The microcytic anemia mutation arose spontaneously and was identified in the F2 hybrid progeny from a cross between C57BL/6J and DBA/2J at The Jackson Laboratory.  A surviving mutant was outcrossed to a C3H/J inbred and the obligate heterozygotes were intercrossed. Subsequent full sibling matings with the line maintained segregating for the mutation yielded the MK/Re inbred strain, which reached generation F24 in 1969, F43 in 1976, and F71 in 1986 at which point it had passed into the colony of Dr. Seldon Bernstein. In 1991 this strain was cryopreserved by Dr. Jane Barker from intercrosses of heterozygotes and homozygotes at generation F84. This bankstock was recovered and re-frozen in the year 2000 using F85p+F6 heterozygous and homozygous breeders. This secondary bankstock was recovered and re-frozen by in vitro fertilization in 2007 using homozygous males and heterozygous or wild-type females at generation F85p+F7p+F1. The mice were backcrossed the to 129S6 to N26.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
129S6.MK-Slc11a2mk/Mdf/Mmnc 129S6 C3H/HeJ 2466 0 116 0


Suggested Control Mice: Wild-type littermates

  • Cell Biology
  • Hematology
  • Models for Human Disease

Donor: Mark Fleming, Ph.D., The Children's Hospital

Primary Reference:

Fleming MD, Trenor CC 3rd, Su MA, Foernzler D, Beier DR, Dietrich WF, Andrews NC. Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene. Nat Genet. 1997 Aug;16(4):383-6. (Medline PMID:9241278).

Canonne-Hergaux F, Fleming MD, Levy JE, Gauthier S, Ralph T, Picard V, Andrews NC, Gros P. The Nramp2/DMT1 iron transporter is induced in the duodenum of microcytic anemia mk mice but is not properly targeted to the intestinal brush border.  Blood. 2000 Dec 1;96(12):3964-70. (Medline PMID: 11090085). 

Fleming MD, Romano MA, Su MA, Garrick LM, Garrick MD, Andrews NC.  Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport.  Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1148-53.  (Medline PMID: 9448300).

Colony and Husbandry Information

Homozygous mutants have decreased viability, and few survive to weaning.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Coat color: Brown

Other:

MMRRC Breeding System: Sib-mating

Breeding Scheme(s):

  • Heterozygous female x Heterozygous male siblings
  • Heterozygous female x Homozygouse male siblings

Generation: N26

Overall Breeding Performance:

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: No Yes Surviving homozygous males can be bred.
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Average litter size: Unknown

Recommended wean age: 3weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
036294-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
036294-UNC-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.