Strain Detail Sheet

Strain Name    :

B6.Cg-Tyrp1b-c Tyrc-ch Dctslt/Mmmh

Stock Number :

000363-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tyrc-ch
Name: chinchilla
Chromosome: 7; 44.0 cM
Alteration at locus: Spontaneous Mutation
Allele Symbol: Dctslt
Name: slaty
Chromosome: 14; 58.0 cM
Alteration at locus: Spontaneous Mutation
Allele Symbol: Tyrp1b-c
Name: brown cordovan
Alteration at locus: Spontaneous Mutation
Gene Symbol: Dct
Name: dopachrome tautomerase
Chromosome: 14
Alteration at locus: Spontaneous Mutation
Gene Symbol: Tyr
Name: tyrosinase
Chromosome: 7
Alteration at locus: Spontaneous Mutation
Gene Symbol: Tyrp1
Name: tyrosinase-related protein 1
Chromosome: 4
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
Multiple mutations- see the gene links above for mutation descriptions.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Tyrp1b-cJ: Homozygous recessive mutant on a nonagouti/black background are chocolate brown and show altered melanin granule morphology. Semidominant mutants result in melanocyte degeneration causing reduced pigmentation and progressive hearing loss.

Tyrc-ch: The mutation in the chinchilla allele was found to be a G to A point mutation that results in an amino acid change at position 464 from alanine to threonine. Mutation reduces the intensity of phaeomelanin more than eumelanin resulting in a coat color described as a medium shade of sepia.

Dctslt: Sequence analysis showed this allele involves a G to A transition at base 985 which results in a missense mutation. With non agouti shows dilution from black to dark gray/brown. Ears slightly yellow.

Strain of Origin: Muliple

Strain genetic background: C57BL/6

Strain Development: Mice from congenic strains carrying individual mutations bred together to combine all three mutations in one stock.

Suggested Control Mice:

  • C57BL/6

Research Applications

  • Developmental Biology
  • Mammalian Pigmentation

Strain Origin

Donor: Lynn Lamoreux, Ph.D., Texas A & M University

Primary Reference:

  • Sweet HO, Remutations at The Jackson Laboratory, Mouse Genome 1993;91(4):862-65 (Medline PMID: Not Available)
  • Beermann F, Ruppert S, Hummler E, Bosch FX, Muller G, Ruther U, Schutz G. Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice. EMBO J. 1990 Sep;9(9):2819-26. (Medline PMID: 2118105 )
  • Jackson IJ, Chambers DM, Tsukamoto K, Copeland NG, Gilbert DJ, Jenkins NA, Hearing V. A second tyrosinase-related protein, TRP-2, maps to and is mutated at the mouse slaty locus. EMBO J. 1992 Feb;11(2):527-35. (Medline PMID: 1537334)

Colony and Husbandry Information

Special Considerations

Feed 9% fat breeder diet.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Brown coat; ears are slighly yellow.

Other:


Breeding

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

  • Homozygous female x Homozygous male

Generation: N10+ for all three mutations prior to mating together and selecting animals homozygous at all three loci and F5+ As of: 4/20/2004

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: unknown unknown

Average litter size: 6

Recommended wean age: 4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000363-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000363-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000363-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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