Strain Name:
B6.Cg-Tyrp1b-c Tyrc-ch Dctslt/Mmmh
Stock Number:
000363-MU
Citation ID:
RRID:MMRRC_000363-MU
Other Names:
tyrosinase-related protein 1, tyrosinase
Major Collection:

Strain Information

Tyrc-ch
Name: tyrosinase; chinchilla
Synonyms: cr, cch
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Spontaneous Mutation
Dctslt
Name: dopachrome tautomerase; slaty
Synonyms: slt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Spontaneous Mutation
Tyrp1b-c
Name: tyrosinase-related protein 1; brown cordovan
Synonyms: Tyrp1b-cJ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Spontaneous Mutation
Dct
Name: dopachrome tautomerase
Synonyms: TRP2, Tyrp-2, tyrosinase-related protein-2, TRP-2, Tyrp2
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Spontaneous Mutation
NCBI: 13190
VEGA: 14
HGNC: HGNC:2709
Homologene: 1447
Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Spontaneous Mutation
NCBI: 22173
Homologene: 30969
Tyrp1
Name: tyrosinase-related protein 1
Synonyms: TRP1, TRP-1, Oca3, isa, Tyrp
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Spontaneous Mutation
NCBI: 22178
Homologene: 464
Genetic Alterations
Multiple mutations- see the gene links above for mutation descriptions.
Genotype Determination
Phenotype
Tyrp1b-cJ: Homozygous recessive mutant on a nonagouti/black background are chocolate brown and show altered melanin granule morphology. Semidominant mutants result in melanocyte degeneration causing reduced pigmentation and progressive hearing loss.

Tyrc-ch: The mutation in the chinchilla allele was found to be a G to A point mutation that results in an amino acid change at position 464 from alanine to threonine. Mutation reduces the intensity of phaeomelanin more than eumelanin resulting in a coat color described as a medium shade of sepia.

Dctslt: Sequence analysis showed this allele involves a G to A transition at base 985 which results in a missense mutation. With non agouti shows dilution from black to dark gray/brown. Ears slightly yellow.
Mammalian Phenotype Terms
Allelic Composition: Tyrc-ch/Tyrc-ch (Genetic Background: involves: C57BL/6 )

Allelic Composition: Tyrc-ch/Tyrc-ch38H (Genetic Background: involves: 101/H * C3H/HeH )

Allelic Composition: Tyrc-42H/Tyrc-ch (Genetic Background: involves: 101/H * C3H/HeH )

Allelic Composition: Tyrc-43H/Tyrc-ch (Genetic Background: involves: 101/H * C3H/HeH )

Allelic Composition: Tyrc-112K/Tyrc-ch (Genetic Background: involves: 101/R1 * C3Hf/R1 * St.2A )

Allelic Composition: Tyrc-40H/Tyrc-ch (Genetic Background: involves: C3H/HeH )

Allelic Composition: Tyrc-a/Tyrc-ch (Genetic Background: involves: C3H/HeJ )

  • pigmentation
  • integument
Allelic Composition: Tyrcm1OR/Tyrc-ch (Genetic Background: involves: C3H/Rl )

Allelic Composition: Dctslt/Dctslt (Genetic Background: STOCK Grem1ld-J Atrnmg )

Allelic Composition: Tyrp1b-c/Tyrp1b-c (Genetic Background: involves: C57BL/10 * DBA/1 )

  • pigmentation
  • integument
MeSH Terms
  • Albinism/enzymology
  • Albinism/genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Catechol Oxidase/genetics
  • Genes
  • Hair Color/genetics
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Molecular Sequence Data
  • Monophenol Monooxygenase/genetics
  • Mutation
  • Oligonucleotide Probes
  • Plasmids
  • Restriction Mapping
  • Chromosome Mapping
  • Crosses, Genetic
  • DNA/genetics
  • DNA/isolation & purification
  • Epidermal Growth Factor/genetics
  • Female
  • Intramolecular Oxidoreductases
  • Isomerases/genetics
  • Male
  • Membrane Glycoproteins
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Muridae
  • Oxidoreductases
  • Polymerase Chain Reaction
  • Proteins/genetics
  • RNA, Messenger/genetics
  • Sequence Homology, Nucleic Acid
Strain Development
Mice from congenic strains carrying individual mutations bred together to combine all three mutations in one stock.
Suggested Control Mice

C57BL/6

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
  • Mammalian Pigmentation
Donor
Lynn Lamoreux, Ph.D., Texas A & M University
Primary Reference

Sweet HO, Remutations at The Jackson Laboratory, Mouse Genome 1993;91(4):862-65 (Medline PMID: Not Available)

Beermann F, Ruppert S, Hummler E, Bosch FX, Muller G, Ruther U, Schutz G. Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice. EMBO J. 1990 Sep;9(9):2819-26. (Medline PMID: 2118105)

Jackson IJ, Chambers DM, Tsukamoto K, Copeland NG, Gilbert DJ, Jenkins NA, Hearing V. A second tyrosinase-related protein, TRP-2, maps to and is mutated at the mouse slaty locus. EMBO J. 1992 Feb;11(2):527-35. (Medline PMID: 1537334)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Brown coat; ears are slighly yellow.
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Homozygous female x Homozygous male
Generation
N10+ for all three mutations prior to mating together and selecting animals homozygous at all three loci and F5+ As of: 4/20/2004
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000363-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000363-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000363-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.