Strain Name:
C57BL/6-Hnrnpllthdr/Mmucd
Stock Number:
036418-UCD
Citation ID:
RRID:MMRRC_036418-UCD
Other Names:
Thunder, Hnrpllthdr, C57BL/6JSfdAnu-Hnrnpllthdr/AnuApb

Strain Information

Hnrnpllthdr
Name: heterogeneous nuclear ribonucleoprotein L-like; thunder
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
Hnrnpll
Name: heterogeneous nuclear ribonucleoprotein L-like
Synonyms: Hnrpll, 2810036L13Rik, 2510028H02Rik
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 72692
Homologene: 26701
Genetic Alterations
The thunder mutation is an ENU-induced T to A change at nucleotide 683 in Exon 2 resulting in an amino acid change from Valine to Aspartic acid at position 136.
Phenotype
Homozygous: Low T cell numbers which are hyperactivated. By flow cytometric staining of blood, initially detected a strain with CD4 T cells decreased to 25% of wild-type. Further analysis revealed entirely normal thymic development and single positive cells, but a marked reduction in the number of peripheral T cells especially na ve CD4 and CD8 cells, and poor survival of thunder T cells upon adoptive transfer. During this analysis, it was found that thunder thymocytes stain with the RA3-6B2 antibody that selectively recognizes the CD45RABC B220 isoform normally restricted to B cells. Staining with antibodies to CD45RA, B or C showed a 50-fold increase in staining on all thymocytes and peripheral T cells, as well as macrophages and dendritic cells, so that the staining intensity matched that on B cells. Amplification of cDNA with primers spanning CD45 exons 4-6 showed a dramatic increase in the inclusion of these exons in thymocyte CD45 mRNA, establishing that the thunder mutation interferes with the normal CD45 exon 4-6 silencing process.
Mammalian Phenotype Terms
Allelic Composition: Hnrnpllthdr/Hnrnpllthdr (Genetic Background: C57BL/6JSfdAnu-Hnrnpllthdr/Anu )

MeSH Terms
  • Alternative Splicing/genetics
  • Amino Acid Sequence
  • Animals
  • Exons/genetics
  • Heterogeneous-Nuclear Ribonucleoproteins/genetics
  • Heterogeneous-Nuclear Ribonucleoproteins/immunology
  • Heterogeneous-Nuclear Ribonucleoproteins/metabolism
  • Immunologic Memory/genetics
  • Leukocyte Common Antigens/immunology
  • Leukocyte Common Antigens/metabolism
  • Mice
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Mutation, Missense/genetics
  • Protein Isoforms/genetics
  • Protein Isoforms/immunology
  • Protein Isoforms/metabolism
  • RNA/genetics
  • RNA/immunology
  • T-Lymphocyte Subsets/immunology
  • T-Lymphocyte Subsets/metabolism
  • Alternative Splicing/immunology
  • Base Sequence
  • Cell Survival/genetics
  • Cell Survival/immunology
  • Leukocyte Common Antigens/biosynthesis
  • Leukocyte Common Antigens/genetics
  • Leukocyte Common Antigens/physiology
  • Lymphopenia/enzymology
  • Lymphopenia/genetics
  • Lymphopenia/immunology
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mutation, Missense
  • Pedigree
  • Protein Isoforms/biosynthesis
  • Protein Isoforms/physiology
  • Receptor-Like Protein Tyrosine Phosphatases, Class 4/biosynthesis
  • Receptor-Like Protein Tyrosine Phosphatases, Class 4/genetics
  • Receptor-Like Protein Tyrosine Phosphatases, Class 4/physiology
  • Receptors, Antigen, T-Cell/genetics
  • Receptors, Antigen, T-Cell/physiology
  • Signal Transduction/genetics
  • Signal Transduction/immunology
  • T-Lymphocyte Subsets/enzymology
  • T-Lymphocyte Subsets/pathology
  • Exons
  • Heterogeneous-Nuclear Ribonucleoproteins/physiology
  • Killer Cells, Natural/immunology
  • T-Lymphocytes/immunology
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Chris Goodnow, Ph.D., F.A.A., F.R.S., Australian National University.
Primary Reference
  • Goodnow C. Record for "thunder", NHMRC Australian Phenome Bank.
  • Wu Z, Jia X, de la Cruz L, Su XC, Marzolf B, Troisch P, Zak D, Hamilton A, Whittle B, Yu D, Sheahan D, Bertram E, Aderem A, Otting G, Goodnow CC, Hoyne GF. Memory T cell RNA rearrangement programmed by heterogeneous nuclear ribonucleoprotein hnRNPLL. Immunity. 2008 Dec 19;29(6):863-75. doi: 10.1016/j.immuni.2008.11.004. (Medline PMID: 19100700).
  • Wu Z, Yates AL, Hoyne GF, Goodnow CC. Consequences of increased CD45RA and RC isoforms for TCR signaling and peripheral T cell deficiency resulting from heterogeneous nuclear ribonucleoprotein L-like mutation. ;J Immunol. 2010 Jul 1;185(1):231-8. doi: 10.4049/jimmunol.0903625. Epub 2010 May 26. (Medline PMID: 20505149).
  • Yabas M, Godfrey DI, Goodnow CC, Hoyne GF. Differential requirement for the CD45 splicing regulator hnRNPLL for accumulation of NKT and conventional T cells. PLoS One. 2011;6(11):e26440. doi: 10.1371/journal.pone.0026440. Epub 2011 Nov 4. (Medline PMID: 22073166).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Other or uncertain
Breeding Scheme(s)
  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Not specified
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036418-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.