Strain Name:
C57BL/6-Zap70mrtle/Mmucd
Stock Number:
036422-UCD
Citation ID:
RRID:MMRRC_036422-UCD
Other Names:
Mr T-Less, C57BL/6SfdAnu-Zap70mrtle/AnuApb

Strain Information

Zap70mrtle
Name: zeta-chain (TCR) associated protein kinase; mr t-less
Synonyms: Zap70mrt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Zap70
Name: zeta-chain (TCR) associated protein kinase
Synonyms: Srk, TZK, ZAP-70
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22637
Homologene: 839
Genetic Alterations
The Mr T-Less mutation is an ENU-induced T to C change at nucleotide 1601 in Exon 11 resulting in an amino acid change from Tryptophan to Arginine at position 504.
Genotype Determination
Phenotype
Homozygous: Identified by severe reduction in T cell numbers in blood.B cells are normalBoth CD4? and CD8? T cell populations were almost completely absent in peripheral blood. Therefore, no ?? T cells but have increase in ?? T cellsNormal numbers of DN and DP T cells. Implies that have block at DP stage and mice unable to perform positive thymocyte selection.Intrinsic to T cells not microenvirnment.Cells unable to pass thymic selection steps.Cells unable to up-regulate TCRReduced signalling through TCR.Zap70 protein expression of mrt/mrt thymocytes was 25% of wildtype thymocytes.Kinase domain affected by mutation, kinase assays not performedHeterozygous mice, mrt/+ have normal levels of CD4? and CD8? thymocytes but Zap70 protein expression is 2 fold lower.Unlike cells devoid of ZAP-70, mrtless thymocytes showed partial induction of CD5 and CD69, and were sensitive to TCR stimulation with a dose-response shifted approximately 10-fold. However, essentially no T cells were able to compensate for the mrtless mutation and mature beyond the CD4? CD8? stage.
Mammalian Phenotype Terms
Allelic Composition: Zap70mrtle/Zap70mrtle (Genetic Background: C57BL/6JSfdAnu-Zap70mrtle/Apb )

Allelic Composition: Zap70mrtle/Zap70mrtle (Genetic Background: involves: C57BL/6JSfdAnu )

Allelic Composition: Zap70mrtle/Zap70rps (Genetic Background: involves: C57BL/6 * C57BL/6JSfdAnu )

Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Chris Goodnow, Ph.D., F.A.A., F.R.S., Australian National University.
Primary Reference
  • Goodnow C. Record for "Mr T-Less", NHMRC Australian Phenome Bank.
  • Siggs OM, Miosge LA, Yates AL, Kucharska EM, Sheahan D, Brdicka T, Weiss A, Liston A, Goodnow CC. Opposing functions of the T cell receptor kinase ZAP-70 in immunity and tolerance differentially titrate in response to nucleotide substitutions. Immunity. 2007 Dec;27(6):912-26. (Medline PMID: 18093540).
  • Siggs OM, Yates AL, Schlenner S, Liston A, Lesage S, Goodnow CC. A ZAP-70 kinase domain variant prevents thymocyte-positive selection despite signalling CD69 induction. Immunology. 2014 Apr;141(4):587-95. (Medline PMID: 24266404).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
MMRRC Breeding System
Other or uncertain
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036422-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.