Strain Name:
B6.Cg-Sagtm1Jnc Tg(Rho-Sag*)50Seva/Mmjax
Stock Number:
036458-JAX
Citation ID:
RRID:MMRRC_036458-JAX

Gene Information

Sagtm1Jnc
Name: S-antigen, retina and pineal gland (arrestin); targeted mutation 1, Jeannie Chen
Synonyms: Arrb1tm1Jnc, arr1-, arrestin-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Sag
Name: S-antigen, retina and pineal gland (arrestin)
Synonyms: A930001K18Rik, rod arrestin, Arr1, arrestin 1, visual arrestin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 20215
Homologene: 455
Rho
Name: rhodopsin
Synonyms: Red Opsin, L opsin, LWS opsin, Long Wavelength Sensitive opsin, Rod Opsin, RP4, Opn2, opsin 2, Ops, Noerg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
NCBI: 212541
Homologene: 68068
Tg(Rho-Sag*)50Seva
Name: transgene insertion 50, Vsevolod Gurevich
Synonyms: 3A-50, Tg(Sag*3A50)1Seva, Tg(Rho-Sag*)1Seva
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The transgenic construct contains three amino acid substitutions (predictions based on NM_009118.2: p.Leu374Ala, Val375Ala, and Phe376Ala; nucleic acid information not available) introduced into the mouse retinal S-antigen (Sag) coding sequence under the direction of the mouse rhodopsin promoter. Line 1 and 2 with moderate and high expression were generated from founders 3A50 and 3A240, respectively.

For the Sag knockout, part of the 5' promoter and exons 1 and 2 were replaced with a neomycin resistance cassette via homologous recombination.
ES Cell Line
Sag knockout allele: unspecified ES cell line derived from "129S/SvEv"
Phenotype

Homozygous: Not evaluated.

Hemizygous: Expresses constitutively active arrestin-1-3A mutant (that binds unphosphorylated Rh* much better than WT arrestin-1) at the level of ~50% of WT. Mutant substitutes WT arrestin-1 on knockout background. The expression of this mutant facilitates photoreceptor recovery in the absence of rhodopsin phosphorylation in rhodopsin kinase knockout mice and in mice expressing mutant rhodopsin with 0, 1, or 2 phosphorylation sites on rhodopsin knockout background.

Mammalian Phenotype Terms
Allelic Composition: Sagtm1Jnc/Sagtm1Jnc (Genetic Background: involves: 129S/SvEv )

MeSH Terms
  • Adenosine Triphosphate/deficiency
  • Animals
  • Arrestin/metabolism
  • Binding Sites/drug effects
  • Binding Sites/physiology
  • Binding Sites/radiation effects
  • Blotting, Western/methods
  • Cytoskeleton/metabolism
  • Dark Adaptation
  • Deoxyglucose/pharmacology
  • Energy Metabolism/physiology
  • Enzyme Activation/physiology
  • Enzyme Activation/radiation effects
  • Eye Proteins
  • Fluorescent Antibody Technique
  • G-Protein-Coupled Receptor Kinase 1
  • Glucose/pharmacology
  • Green Fluorescent Proteins/metabolism
  • Hydroxylamine/pharmacology
  • In Vitro Techniques
  • Light
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microtubules/metabolism
  • Mutagenesis/physiology
  • Phosphorylation
  • Potassium Cyanide/pharmacology
  • Protein Binding/physiology
  • Protein Kinases/deficiency
  • Protein Transport/drug effects
  • Protein Transport/physiology
  • Protein Transport/radiation effects
  • Retina/cytology
  • Retinal Rod Photoreceptor Cells/drug effects
  • Retinal Rod Photoreceptor Cells/metabolism
  • Retinal Rod Photoreceptor Cells/radiation effects
  • Rhodopsin/metabolism
  • Rod Opsins/metabolism
  • Time Factors
  • Transducin/metabolism
  • Arrestin/genetics
  • Electroretinography
  • G-Protein-Coupled Receptor Kinase 1/genetics
  • G-Protein-Coupled Receptor Kinase 1/metabolism
  • Mice, Knockout
  • Mutation
  • Retinal Rod Photoreceptor Cells/cytology
  • Rhodopsin/genetics
  • Arrestins/biosynthesis
  • Arrestins/genetics
  • Darkness
  • Photoreceptor Cells, Vertebrate/cytology
  • Photoreceptor Cells, Vertebrate/physiology
  • Rod Cell Outer Segment/metabolism
  • Rod Cell Outer Segment/ultrastructure
Strain Development
Transgene-positive mice developed on C57BL/6 background were back-crossed to C57BL/6 and arrestin-1 knockout mice, Sagtm1Jnc, on C57BL/6 background. Backcrossed with C57BL/6 for >5 years prior to donation to the MMRRC.
Suggested Control Mice
Wild-type littermates or C57BL/6
  • Apoptosis
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Diabetes/Obesity
    • Endocrine Deficiency
    • Hematology
    • Immunology and Inflammation
    • Internal Organ
    • Metabolism
    • Models for Human Disease
    • Neurobiology
    • Reproduction
    • Research Tools
    • Sensorineural
    • Virology
    Donor
    Vsevolod Gurevich, Ph.D., Vanderbilt University Medical Center.
    Jeannie Chen, Ph.D., Zilkha Neurogenetic Institute.
    Primary Reference

    Nair KS, Hanson SM, Mendez A, Gurevich EV, Kennedy MJ, Shestopalov VI, Vishnivetskiy SA, Chen J, Hurley JB, Gurevich VV, Slepak VZ. Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions. Neuron. 2005 May 19;46(4):555-67. (Medline PMID: 15944125).

    Song X, Vishnivetskiy SA, Gross OP, Emelianoff K, Mendez A, Chen J, Gurevich EV, Burns ME, Gurevich VV. Enhanced arrestin facilitates recovery and protects rods lacking rhodopsin phosphorylation. Curr Biol. 2009 Apr 28;19(8):700-5. Epub 2009 Apr 9. (Medline PMID: 19361994).

    Song X, Vishnivetskiy SA, Seo J, Chen J, Gurevich EV, Gurevich VV. Arrestin-1 expression level in rods: balancing functional performance and photoreceptor health. Neuroscience. 2011 Feb 3;174:37-49. Epub 2010 Nov 12. (Medline PMID: 21075174).

    Colony and Husbandry Information

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    Backcross
    • C57BL/6 female x Hemizygous male or reciprocal mating
    Generation
    N10+ (C57BL/6)
    Overall Breeding Performance
    Good
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Unknown Unknown
    Homozygotes are fertile: Unknown Unknown
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Bred to Homozygosity
    No

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    036458-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
    036458-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.