Strain Name:
B6.Cg-Sagtm1Jnc Tg(Rho-Sag)120Seva/Mmmh
Stock Number:
036461-MU
Citation ID:
RRID:MMRRC_036461-MU
Other Names:
WT-120

Strain Information

Sagtm1Jnc
Name: S-antigen, retina and pineal gland (arrestin); targeted mutation 1, Jeannie Chen
Synonyms: arrestin-, Arrb1tm1Jnc, arr1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Sag
Name: S-antigen, retina and pineal gland (arrestin)
Synonyms: visual arrestin 1, arrestin 1, A930001K18Rik, Arr1, rod arrestin
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 20215
Homologene: 455
Rho
Name: rhodopsin
Synonyms: Red Opsin, Opn2, RP4, Noerg1, Rod Opsin, Long Wavelength Sensitive opsin, LWS opsin, L opsin, Ops, opsin 2
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 212541
Homologene: 68068
Tg(Rho-Sag)120Seva
Name: transgene insertion 120, Vsevolod Gurevich
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
A full-length mouse cone arrestin cDNA was expressed under the direction of the mouse rhodopsin promoter. Arrestin was at a level of about 120X the level in wild-type mice.

For the Sag knockout, part of the 5' promoter and exons 1 and 2 were replaced with a neomycin resistance cassette via homologous recombination.
Genotype Determination
ES Cell Line
Not specified
Phenotype

Homozygous: Not evaluated

Heterozygous: Expresses higher than normal level of WT arrestin-1 that can be regulated by crossing with arrestin-1 wild-type, heterozygous, or homozygous mice.

Mammalian Phenotype Terms
Allelic Composition: Sagtm1Jnc/Sagtm1Jnc (Genetic Background: involves: 129S/SvEv )

MeSH Terms
  • Animals
  • Arrestins/biosynthesis
  • Arrestins/genetics
  • Darkness
  • Electroretinography
  • Mice
  • Mice, Transgenic
  • Photoreceptor Cells, Vertebrate/cytology
  • Photoreceptor Cells, Vertebrate/physiology
  • Retinal Rod Photoreceptor Cells/cytology
  • Retinal Rod Photoreceptor Cells/metabolism
  • Rod Cell Outer Segment/metabolism
  • Rod Cell Outer Segment/ultrastructure
  • Arrestins/physiology
  • Blotting, Western
  • Electrophoresis, Polyacrylamide Gel
  • Electrophysiology
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression
  • Light
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phosphorylation
  • Photic Stimulation
  • Retinal Cone Photoreceptor Cells/physiology
  • Retinal Cone Photoreceptor Cells/radiation effects
  • Retinal Rod Photoreceptor Cells/physiology
  • Retinal Rod Photoreceptor Cells/radiation effects
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhodopsin/metabolism
  • Vision, Ocular/physiology
  • beta-Arrestin 1
  • beta-Arrestins
  • Alternative Splicing/physiology
  • Arrestin/biosynthesis
  • Arrestin/genetics
  • Arrestin/physiology
  • Genetic Variation/physiology
  • In Vitro Techniques
  • Mice, Inbred DBA
  • Photic Stimulation/methods
  • Retina/metabolism
  • Rhodopsin/genetics
Strain Development
The transgene was microinjected into fertilized C57BL/6 oocytes and founder mice were crossed to C57BL/6 mice for an unknown number of generations. Founder line WT-120 expresses arrestin at 120% of wild-type. Mice were crossed to B6.129S-Sagtm1Jnc mice to generate mice that are heterozygous for the targeted mutation and hemizygous for the transgene. The colony was maintained as a backcross to C57BL/6. Upon arrival, mice were bred to C57BL/6J mice to establish the colony.
Suggested Control Mice
Wild-type littermates or C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
  • Sensorineural
  • Virology
Donor
Vsevolod Gurevich, Ph.D., Vanderbilt University Medical Center.
Jeannie Chen, Ph.D., Zilkha Neurogenetic Institute.
Primary Reference

Song X, Vishnivetskiy SA, Seo J, Chen J, Gurevich EV, Gurevich VV. Arrestin-1 expression level in rods: balancing functional performance and photoreceptor health. Neuroscience. 2011 Feb 3;174:37-49. Epub 2010 Nov 12. (Medline PMID: 21075174).

Chan S, Rubin WW, Mendez A, Liu X, Song X, Hanson SM, Craft CM, Gurevich VV, Burns ME, Chen J. Functional comparisons of visual arrestins in rod photoreceptors of transgenic mice. Invest Ophthalmol Vis Sci. 2007 May;48(5):1968-75. (Medline PMID: 17460248).

Burns ME, Mendez A, Chen CK, Almuete A, Quillinan N, Simon MI, Baylor DA, Chen J. Deactivation of phosphorylated and nonphosphorylated rhodopsin by arrestin splice variants. J Neurosci. 2006 Jan 18;26(3):1036-44. (Medline PMID: 16421323).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating and Backcross:
  • Mice are distributed heterozygous for the knockout Sagtm1Jnc and hemizygous or negative for the transgene Tg(Rho-Sag)120Seva. The ideal research animal is likely wild-type for the arrestin gene/transgene positive.
Breeding Scheme(s)

Sib-mating and Backcross

  • Heterozygous KO/Hemizygous transgene female x Heterozygous KO/Hemizygous transgene female (yields mice with either 0, 1 or 2 copies of transgene)
  • Heterozygous KO/Hemizygous transgene female x Heterozygous KO/transgene negative female (yields mice with either 0 or 1 copy of transgene)
  • C57BL/6 female x Wild-type for the arrestin gene/Hemizygous transgene male or reciprocal mating
Generation
N10+ (C57BL/6)
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Bred to Homozygosity
No

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036461-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036461-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.