Strain Name:
B6.129S6-Pdx1tm2Mgn/Mmnc
Stock Number:
036462-UNC
Citation ID:
RRID:MMRRC_036462-UNC
Other Names:
Pdx1.CFP

Gene Information

Allele Symbol: Pdx1tm2Mgn (Mus musculus (mouse))
Name: pancreatic and duodenal homeobox 1; targeted mutation 2, Mark A Magnuson
Synonyms: Pdx1
Chromosome: 5
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:5438568
Gene Symbol: Pdx1 (Mus musculus (mouse))
Name: pancreatic and duodenal homeobox 1
Synonyms: STF-1, IDX-1, IPF-1, Mody4, pdx-1, Ipf1
Chromosome: 5
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:102851
Genetic Alterations
Modifications were introduced within Exon 1. Native gene sequences contained in the Pdx-1 basal exchange plasmid were modified by introducing Cerulean.3X SV40 NLS.Beta Globin PolyA sequence within introduced FseI, PacI, PmeI and MluI sites. The generation of a Pdx-1 selectable marker will enable the isolation of pancreatic lineage progenitors.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1 derived from 129S6/SvEvTac
    Phenotype

    Homozygous: Not evaluated.

    Heterozygous: This mouse is heterozyogous null with respect to expression of Pdx1.

    Mammalian Phenotype Terms
    Allelic Composition: Pdx1tm2Mgn/Pdx1+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL )

    MeSH Terms
    • Alleles
    • Animals
    • Cell Differentiation
    • Embryonic Stem Cells/cytology
    • Embryonic Stem Cells/metabolism
    • Endoderm/embryology
    • Endoderm/metabolism
    • Female
    • Flow Cytometry/methods
    • Gene Expression Regulation, Developmental
    • Gene Regulatory Networks
    • Gene Targeting
    • Genes, Reporter
    • Green Fluorescent Proteins/genetics
    • Green Fluorescent Proteins/metabolism
    • Homeodomain Proteins/genetics
    • Homeodomain Proteins/metabolism
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Microscopy, Fluorescence
    • Mutagenesis, Insertional/methods
    • Pancreas/cytology
    • Pancreas/embryology
    • Recombinases/genetics
    • Recombinases/metabolism
    • Trans-Activators/genetics
    • Trans-Activators/metabolism
    Strain Development
    Pdx1tm1.2(CFP)Mgn is from a mixed genetic background of both 129S6 and C57BL/6J. Donor reported that mice shipped to MMRRC strain were 96.9% congenic on C57BL/6J by SNP analysis.


    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129S6-Pdx1tm2Mgn/Mmnc 36462 C57BL/6J 129S6/SvEvTac 2583 0 1 0
    Suggested Control Mice
    Wild-type littermates or C57BL/6J
    • Diabetes/Obesity
    • Internal Organ
    • Research Tools
    Donor
    Mark Magnuson, Vanderbilt University.
    Primary Reference

    Potter LA, Choi E, Hipkens SB, Wright CV, Magnuson MA. An RMCE-derived cyan fluorescent protein reporter allele for Pdx1. Genesis. 2012 Apr;50(4):384-92. Epub 2012 Jan 6. (Medline PMID: 21913313).

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    • We have been performing backcross mating into C57Bl/6J for more than 10 generations.
    Generation
    N>20
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Unknown Unknown
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    036462-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.