Strain Name:
B6;129S4-Sash1Gt(S2-5C1)Sor/Mmucd
Stock Number:
036479-UCD
Citation ID:
RRID:MMRRC_036479-UCD
Other Names:
S2-5C1
Major Collection:

Strain Information

Sash1Gt(S2-5C1)Sor
Name: SAM and SH3 domain containing 1; gene trap S2-5C1, Philippe Soriano
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Gene Trap
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: 2500002E12Rik, A330076K04Rik, 1100001C18Rik
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Gene Trap
NCBI: 70097
Homologene: 69182
Genetic Alterations
This sequence was obtained by 5'RACE PCR from a 129S4/SvJaeSor mouse embryonic stem (ES) cell line (AK7) with an insertional mutation from gene trap vector ROSAFARY.
Genotype Determination
ES Cell Line
AK7.1 derived from 129S4/SvJaeSor
Phenotype
These mice were not phenotyped prior to cryopreservation, nor were they mated to produce homozygous offspring, therefore the homozygous phenotype is unknown.
MeSH Terms
  • Animals
  • Blastocyst/physiology
  • Chimera
  • Embryo, Mammalian/physiology
  • Embryonic and Fetal Development
  • Female
  • Genetic Vectors
  • Indicators and Reagents
  • Kanamycin Kinase
  • Male
  • Mice
  • Mice, Inbred C57BL/genetics
  • Mice, Inbred Strains/genetics
  • Mutagenesis, Insertional/methods
  • Organ Culture Techniques/methods
  • Phosphotransferases (Alcohol Group Acceptor)/biosynthesis
  • Phosphotransferases (Alcohol Group Acceptor)/genetics
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins/biosynthesis
  • Retroviridae/genetics
  • Stem Cells/physiology
  • beta-Galactosidase/biosynthesis
  • beta-Galactosidase/genetics
  • Cloning, Molecular
  • DNA, Complementary/metabolism
  • Electroporation
  • Embryo, Mammalian/cytology
  • Genes, Reporter
  • Genetic Techniques
  • Genotype
  • Mice, Inbred C57BL
  • Models, Genetic
  • Mutagenesis
  • Mutation
  • Oligonucleotides/chemistry
  • Phenotype
  • Plasmids/metabolism
  • Polymerase Chain Reaction
  • Stem Cells/cytology
Strain Development
The ES cell line was injected into C57BL/6J host blastocysts to generate chimeras. The chimeras were then crossed with C57BL/6J mice to determine germline transmission. The resulting heterozygous males were cryoarchived for distribution.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Research Tools
Donor
Soriano, Philippe, Mount Sinai Medical Center, NY (See Soriano Lab Overview)
Primary Reference
Friedrich G, Soriano P., Insertional mutagenesis by retroviruses and promoter traps in embryonic stem cells. Methods Enzymol 1993;225:681-701.(Medline PMID: 8231879 )
Chen WV, Soriano P. Gene trap mutagenesis in embryonic stem cells. Methods Enzymol. 2003;365:367-86. (Medline PMID: 14696359)

Colony and Husbandry Information

*During development at MMRRC at UCD, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the background strain.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
White-bellied agouti or black
MMRRC Breeding System
Backcross or Sib-mated
Breeding Scheme(s)
  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal
  • Wild-type female x Heterozygous male littermate or reciprocal
Generation
N1F1
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined*
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036479-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036479-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.