Strain Name:
B6.SJL-Tg(Enam-lacZ)1Jcch/Mmucd
Stock Number:
036488-UCD
Citation ID:
RRID:MMRRC_036488-UCD
Other Names:
5.2-Enam-LacZ transgenic mouse

Strain Information

lacZ
Name: beta galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Chromosome:
Enam
Name: enamelin
Synonyms: abte
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13801
HGNC: HGNC:3344
Homologene: 9698
Tg(Enam-lacZ)1Jcch
Name: transgene insertion 1, Jan Hu
Synonyms: 5.2 kb-Enam-LacZ, 5.2 Kb enamelin-LacZ
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The expression of the transgene, NLS LacZ, is tissue and stage specific. Therefore, this transgenic construct allows specific targeting of secretory stage ameloblasts.
Genotype Determination
Phenotype

No abnormalities associated with the developing teeth were observed in this model.

MeSH Terms
  • Ameloblasts/metabolism
  • Amelogenesis/genetics
  • Animals
  • Binding Sites
  • Cell Line, Transformed
  • Core Binding Factor Alpha 1 Subunit/physiology
  • Dental Enamel Proteins/biosynthesis
  • Dental Enamel Proteins/genetics
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Homeodomain Proteins/physiology
  • MSX1 Transcription Factor/physiology
  • Mice
  • Mice, Transgenic
  • Organ Specificity/genetics
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid/physiology
  • Transcription Factors/physiology
  • beta-Galactosidase/genetics
Strain Development
The purified construct was microinjected into (C57BL/6X SJL)F2 eggs to generate transgenic mice. The transgenic founder was crossed with C57BL/6J mice to generate F1. Offspring from F1 generation were futher backcrossed through more that 10 additional generations to make strain congenic on the C57BL/6 background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
  • Research Tools
Donor
Jan Ching Chun Hu, Ph.D., University of Michigan.
Primary Reference

Hu Y, Papagerakis P, Ye L, Feng JQ, Simmer JP, Hu JC. Distal cis-regulatory elements are required for tissue-specific expression of enamelin (Enam). Eur J Oral Sci. 2008 Apr;116(2):113-23.  (Medline PMID: 18353004).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

C57BL/6 female x Hemizygous male or reciprocal mating

Hemizygous female x Hemizygous male littermate

Hemizygous female x Wild-type male littermate or reciprocal

Generation
N10+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036488-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036488-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036488-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.