Strain Name:
B6;129P2-PhipGt(RRJ571)Byg/Mmucd
Stock Number:
036552-UCD
Citation ID:
RRID:MMRRC_036552-UCD
Other Names:
phip
Major Collection:

Strain Information

PhipGt(RRJ571)Byg
Name: pleckstrin homology domain interacting protein; gene trap RRJ571, BayGenomics
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Gene Trap
Phip
Name: pleckstrin homology domain interacting protein
Synonyms: Wdr11, Ndrp, 2810004D21Rik, 4632404O06Rik
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Gene Trap
NCBI: 83946
Homologene: 41209
Genetic Alterations
This clone contains an exon-trap cassette in intron 4 of the Phip gene. To identify the insertion site for the gene-trap, we carried out PCR-based chromosome walking using a series of forward and reverse primers specific to intron 4. The trap is located approximately 1516-bp downstream of exon 4 and contains a Beta-geo cassette.
Genotype Determination
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Phenotype
Homozygous: mice lacking PHIP1, the full-length isoform of PHIP, are born at normal size but suffer a 40% growth deficit by weaning. PHIP1 mutant mice develop hypoglycemia and have an average lifespan of 4–5 weeks. PHIP1-deficient mouse embryonic fibroblasts (MEFs) grow markedly slower than wild-type MEFs, but exhibit normal AKT phosphorylation and an increased cell proliferation in response to IGF-1 treatment. Together these results suggest that PHIP1 regulates postnatal growth in an IGF-1/AKT pathway-independent manner.
Heterozygous: Normal
Mammalian Phenotype Terms
Allelic Composition: PhipGt(RRJ571)Byg/PhipGt(RRJ571)Byg (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

MeSH Terms
  • Animals
  • Cell Proliferation
  • Failure to Thrive/genetics
  • Female
  • Hypoglycemia/genetics
  • Insulin-Like Growth Factor I/metabolism
  • Mice
  • Mice, Mutant Strains
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Proto-Oncogene Proteins c-akt/metabolism
Strain Development
Phip gene-trap mice were generated by micro-injection of mouse embryonic stem cell line RRJ571 (Baygenomics) into C57BL/6J blastocysts. The resulting chimeric male founders were crossed to C57BL/6J females to generate F1 heterozygous (Phip+/−) mice. Phip+/− mice were then intercrossed to generate F2 homozygous Phip−/− mice or embryos.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Diabetes/Obesity
  • Metabolism
Donor
John Schimenti, Ph.D., Cornell University College of Veterinary Medicine.
Primary Reference

Li S, Francisco AB, Han C, Pattabiraman S, Foote MR, Giesy SL, Wang C, Schimenti JC, Boisclair YR, Long Q. The full-length isoform of the mouse pleckstrin homology domain-interacting protein (PHIP) is required for postnatal growth. FEBS Lett. 2010 Sep 24;584(18):4121-7. Epub 2010 Sep 4. (Medline PMID:20816727).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
(Note: if sib-mated, it is possible to get pink-eyed chinchilla, the color of the 129P2/OlaHsd, or other mixed variations.)
Other
Homozygous animals are runty, semi-lethal, and growth-retarded.
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Heterozygous female x Heterozygous male

Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036552-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036552-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
036552-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.