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Homozygous: Pgk2-/- males exhibit normal mating behavior and produce copulatory plugs, but sire very few pups during 3-4 mo. continuous mating trials with two females per male. Fewer pups were produced on the 129 background. On both backgrounds, the Pgk2-/- mice exhibit normal testis histology, sperm counts and sperm ultrastructure. Sperm from Pgk2-/- mice exhibit marked deficiencies in ATP levels and in motility. Female Pgk2-/- mice exhibit normal fertility.Heterozygous: Heterozygous pairs on the B6 background produce litters of normal size with the expected Mendelian ratio of genotypes. In a mating study with FVB/NJ females, heterozygous Pgk2-/- males on the 129 background sired smaller litters than B6 heterozygous males or wild-type males on either background. Previously published studies showed that the wild-type 129 allele of Pgk2 (allele c) has significantly lower activity than the a and b alleles identified in other strains.
A knockout mouse line with the phosphoglycerate kinase-2 (Pgk2) gene deleted by homologous recombination was generated using 129S6/SvEvTac genomic DNA fragments homologous to regions within the endogenous Pgk2 locus on murine chromosome 17. Male chimeras from two targeted ES cell lines (U32 and V8) were mated with C57BL/6NCrl females. Heterozygous (Pgk2+/-) offspring with a mixed genetic background (129S6/SvEvTac and C57BL/6NCrl) were mated to produce homozygous, heterozygous and wild-type males for comparative analysis of fertility and sperm function. Heterozygous F1 males were backcrossed to C57BL/6NCrl females for ten generations until the current background separation was achieved.
Strain Genetic Background Validation:
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, OBrien DA. Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice. Biol Reprod. 2010 Jan;82(1):136-45. Epub 2009 Sep 16. (Medline PMID: 19759366).
Colony Surveillance Program and Current Health Reports
129S6/SvEvTac female x Heterozygous male or reciprocal mating
Heterozygous female x Heterozygous male sibling
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.