Strain Name:
FVB/NTac-Nup210lTg(Gt(ROSA)26Sor-EGFP)130910Eps/Mmmh
Stock Number:
000366-MU
Citation ID:
RRID:MMRRC_000366-MU

Strain Information

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: beta geo, R26, Gtrosa26, Thumpd3as1, Gtrgeo26, ROSA26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Nup210lTg(Gt(ROSA)26Sor-EGFP)130910Eps
Name: transgene insertion 130910, Eric Sandgren
Synonyms: R26-EGFP, ROSA-EGFP
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Mutant mice contain the EGFP transgene under the control of the mouse ROSA 26 promoter. The SalI-BamHI fragment of the mouse ROSA 26 gene was inserted into the SalI/BamHI site of pEGFP-N1 (Clonetech) containing the enhanced green fluorescent protein gene. The SalI-AflI fragment was then excised and microinjected into FVB/N embryos to produce transgenic mice. The transgene insertion site, Nup210l, was later determined via chromosome-walking technique (PMID:20034429).
Genotype Determination
Phenotype
Hemizygous mice exhibit ubiquitous expression of EGFP in embryos and adult mice.
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Consensus Sequence
  • Embryonic and Fetal Development
  • Exons
  • Female
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Genetic Markers
  • Genotype
  • Green Fluorescent Proteins
  • Humans
  • Liver/embryology
  • Liver/metabolism
  • Luminescent Proteins/analysis
  • Luminescent Proteins/genetics
  • Mammary Glands, Animal/embryology
  • Mammary Glands, Animal/metabolism
  • Mice
  • Mice, Transgenic
  • Mutagenesis, Site-Directed
  • Organ Specificity
  • Promoter Regions, Genetic
  • Proteins/genetics
  • RNA, Untranslated
  • Rats
  • Recombinant Fusion Proteins/analysis
  • DNA Primers
  • Green Fluorescent Proteins/genetics
  • Immunohistochemistry
  • Infertility, Male/genetics
  • Male
  • Mutation
  • Spermatogenesis/genetics
  • Transgenes
Strain Development
Founder males were mated to FVB/N females. Founder line 1309-10 had high level expression and was used to produce the R26-EGFP line. Hemizygous transgenic-positive males are mated to FVB/N females to maintain the colony.
Suggested Control Mice
  • Wildtype littermates
  • FVB/NTac
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • This model can be used as a basic research tool in all fields thatrequire cell markers, such as embryonic development andtransplantation studies.
  • Cell Biology
  • Neurobiology
  • Reproduction
  • Research Tools
  • Transplantation
Donor
Eric Sandgren, Ph.D., University of Wisconsin
Primary Reference

Walters EM, Bauer BA, Franklin CL, Evans TJ, Bryda EC, Riley LK, Critser JK.Mutational insertion of a ROSA26-EGFP transgene leads to defects inspermiogenesis and male infertility in mice. Comp Med. 2009 Dec;59(6):545-52.(Medline PMID: 20034429)

Kisseberth WC, Brettingen NT, Lohse JK, Sandgren EP. Ubiquitous expression of marker transgenes in mice and rats. Dev Biol. 1999 Oct 1;214(1):128-38. (Medline PMID: 10491262)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Inbred x Hemizygote
Breeding Scheme(s)
FVB/NTac female x Hemizygous male
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Bred to Homozygosity
No
Average litter size
8
Recommended wean age
3 weeks
Average Pups Weaned
8

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000366-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000366-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000366-MU-RESUS Litter recovered from cryo-archive $2,028.00 / $4,127.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.