Strain Name:
STOCK Foxd3tm3Lby/Mmmh
Stock Number:
036674-MU
Citation ID:
RRID:MMRRC_036674-MU
Other Names:
Foxd3 Flox

Gene Information

Foxd3tm3Lby
Name: forkhead box D3; targeted mutation 3, Patricia A Labosky
Synonyms: Foxd3flox, Foxd3fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
Foxd3
Name: forkhead box D3
Synonyms: Genesis, Hfh2, CWH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
NCBI: 15221
HGNC: HGNC:3804
Homologene: 49239
Genetic Alterations
This is a conditional allele. The entire coding region (one exon) is flanked by loxP sites. A neo with FRT flanking sites remains in the locus.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype

Homozygous: Mice are normal prior to Cre expression.

Heterozygous: Mice are normal prior to Cre expression.

MeSH Terms
  • Animals
  • Body Patterning/physiology
  • Cell Death
  • Cell Differentiation
  • Cell Movement
  • Cell Proliferation
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/physiology
  • Mice
  • Multipotent Stem Cells/cytology
  • Multipotent Stem Cells/physiology
  • Mutation
  • Neural Crest/abnormalities
  • Neural Crest/cytology
  • Neural Crest/embryology
  • Peripheral Nervous System/abnormalities
  • Peripheral Nervous System/embryology
  • Repressor Proteins/genetics
  • Repressor Proteins/physiology
  • Wnt1 Protein/genetics
  • Wnt1 Protein/physiology
  • Biomarkers/metabolism
  • Cell Differentiation/drug effects
  • Cell Division
  • Cell Line
  • Cell Lineage/drug effects
  • Cell Proliferation/drug effects
  • Cell Survival/drug effects
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/drug effects
  • Embryonic Stem Cells/metabolism
  • Forkhead Transcription Factors/metabolism
  • Gene Deletion
  • Gene Expression Regulation, Developmental/drug effects
  • Models, Biological
  • Pluripotent Stem Cells/cytology
  • Pluripotent Stem Cells/drug effects
  • Pluripotent Stem Cells/metabolism
  • Repressor Proteins/metabolism
  • Tamoxifen/analogs & derivatives
  • Tamoxifen/pharmacology
  • Cell Lineage
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/innervation
  • Embryo, Mammalian/metabolism
  • Forkhead Transcription Factors/deficiency
  • Gene Expression Regulation, Developmental
  • Mice, Knockout
  • Multipotent Stem Cells/metabolism
  • Neural Crest/metabolism
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Repressor Proteins/deficiency
Strain Development
Original mutation was introduced into TL1 ES cells (129S6), chimeras were generated and mated to C57BL/6 mice and heterozygous offspring were interbred. We have backcrossed to CD1 a couple generations to improve the robustness of the line.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes/Obesity
Donor
Patricia Labosky, Ph.D., Vanderbilt University Medical Center.
Primary Reference

Teng L, Mundell NA, Frist AY, Wang Q, Labosky PA. Requirement for Foxd3 in Maintenance of Neural Crest Progenitors. Development. 2008 May;135(9):1615-24. Epub 2008 Mar 26. (Medline PMID: 18367558).

Liu Y, Labosky PA. Regulation of ES Cell Self-renewal and Pluripotency by Foxd3. Stem Cells. 2008 Oct;26(10):2475-84. Epub 2008 Jul 24. (Medline PMID: 18653770).

Mundell NA, Labosky PA. Neural Crest Stem Cell Multipotency Requires Foxd3 to Maintain Neural Potential and Repress Mesenchymal Fates. Development. 2011 Feb;138(4):641-52. Epub 2011 Jan 12. (Medline PMID: 21228004).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Brown or White
MMRRC Breeding System
Sib-mating or Outcross to CD1
Breeding Scheme(s)

Heterozygous female x Heterozygous male sibling

Homozygous female x Homozygous male sibling

CD1 female x Heterozygous male or reciprocal mating

Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 12 months
Average litter size
10-14
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036674-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity2
036674-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.