Strain Name    : B6;129S4-Neurod1tm1Jle/Mmucd

Stock Number : 000367-UCD

Other Names   : NeuroD/lacZ Knock-In, B6;129S4-Neurod1tm1Jle/Mmcd

Gene Information

(provided by MGI)
Target: Neurod1tm1Jle
Name: targeted mutation 1, Jacqueline E Lee
Alteration at locus: Knock-In
Target: Neurod1
Name: neurogenic differentiation 1
Chromosome: 2
Alteration at locus: Knock-In
Genetic Alterations:
LacZ-pA-(inverted PKG-neo) cassette was inserted between +1484 (Asp718; within the intron) and +3134 (SpeI; within the 3'UTR) sites. CDS: +1715 to +2788.

Genotype Determination:

ES Cell Line: AK7 derived from B6;129S4

Phenotype

Homozygous phenotype: Postnatal lethal due to neonatal diabetes Pancreas--5% insulin production, no islet formation Gut--no CCK or secretin production Inner ear defects resulting in deafness--no cochlear ganglia and poor vestibular ganglia formation Cerebellar defect resulting in ataxia-- >90% depletion of cerebellar granule cells Hippocampal defects--no dentate gyrus


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Neurod1tm1Jle/Neurod1tm1Jle
        involves: 129S4/SvJaeSor * C57BL/6
  • mortality/aging
    • complete postnatal lethality (MGI Ref ID J:74715)
      • homozygotes die within 5 days after birth due neonatal diabetes
  • homeostasis/metabolism phenotype
    • hyperglycemia (MGI Ref ID J:74715)
      • homozygotes become severely diabetic soon after birth
  • vision/eye phenotype
    • abnormal Muller cell morphology (MGI Ref ID J:51299)
      • after 12 days of culture, explants initiated from mutant retinal tissue at P0 (before the onset of hyperglycemia) show a 3-to 4-fold increase of Muller glia in the inner nuclear layer
      • ectopic Muller cell bodies are occasionally noted in the outer nuclear layer
    • abnormal amacrine cell morphology (MGI Ref ID J:51299)
      • retinal explant cultures exhibit delayed amacrine cell differentiation
      • in contrast, the initial stages of cone, rod and Muller cell differentiation appear unaffected
      • notably, the number of amacrine cells reaches normal levels after 12 days in culture
    • abnormal retinal apoptosis (MGI Ref ID J:51299)
      • a subset of photoreceptors in mutant retinal explants undergo apoptosis after 12 days of culture
    • abnormal retinal bipolar cell morphology (MGI Ref ID J:51299)
      • retinal explant cultures exhibit a 2-fold increase in bipolar cell differentiation
      • notably, overpresentation of bipolar neurons in mutant retina appears to be dependent on gene dosage
  • nervous system phenotype
    • abnormal Muller cell morphology (MGI Ref ID J:51299)
      • after 12 days of culture, explants initiated from mutant retinal tissue at P0 (before the onset of hyperglycemia) show a 3-to 4-fold increase of Muller glia in the inner nuclear layer
      • ectopic Muller cell bodies are occasionally noted in the outer nuclear layer
    • abnormal amacrine cell morphology (MGI Ref ID J:51299)
      • retinal explant cultures exhibit delayed amacrine cell differentiation
      • in contrast, the initial stages of cone, rod and Muller cell differentiation appear unaffected
      • notably, the number of amacrine cells reaches normal levels after 12 days in culture
    • abnormal retinal bipolar cell morphology (MGI Ref ID J:51299)
      • retinal explant cultures exhibit a 2-fold increase in bipolar cell differentiation
      • notably, overpresentation of bipolar neurons in mutant retina appears to be dependent on gene dosage


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Neurod1tm1Jle/Neurod1tm1Jle
        involves: 129S4/SvJaeSor * ICR
  • vision/eye phenotype
    • abnormal retinal apoptosis (MGI Ref ID J:121047)
      • cell death is increased slightly




Strain of Origin: 129/SvJ

Strain genetic background: mixed in C57BL/6J and 129/SvJ

Suggested Control Mice: Wild-type littermates

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Sensorineural

Donor: Jackie Lee, Ph.D., University of Colorado at Boulder

Primary Reference:

  • Miyata T, Maeda T, Lee JE. NeuroD is required for differentiation of the granule cells in the cerebellum and hippocampus. Genes Dev. 1999 Jul 1;13(13):1647-52. (Medline PMID: 10398678)
  • Kim WY, Fritzsch B, Serls A, Bakel LA, Huang EJ, Reichardt LF, Barth DS, Lee JE. NeuroD-null mice are deaf due to a severe loss of the inner ear sensory neurons during development. Development. 2001 Feb;128(3):417-26. (Medline PMID: 11152640)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: agouti

Other:

MMRRC Breeding System: Intrastrain

Breeding Scheme(s):

  • Heterozygous female x Wild type male

Generation: Unknown

Overall Breeding Performance: Normal

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 16 weeks 16 weeks

Average litter size: 6 - 8

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000367-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.