Strain Name:
B6;129S4-Neurod1tm1Jle/Mmucd
Stock Number:
000367-UCD
Citation ID:
RRID:MMRRC_000367-UCD
Other Names:
NeuroD/lacZ Knock-In, B6;129S4-Neurod1tm1Jle/Mmcd

Gene Information

Allele Symbol: Neurod1tm1Jle (Mus musculus (mouse))
Name: neurogenic differentiation 1; targeted mutation 1, Jacqueline E Lee
Synonyms: ND -, Neurod1, NeuroD1:lacZ, Neurod1
Chromosome: 2
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:2385826
Gene Symbol: Neurod1 (Mus musculus (mouse))
Name: neurogenic differentiation 1
Synonyms: Neurod, bHLHa3, BETA2, Nd1
Chromosome: 2
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:1339708
Genetic Alterations
LacZ-pA-(inverted PKG-neo) cassette was inserted between +1484 (Asp718; within the intron) and +3134 (SpeI; within the 3'UTR) sites. CDS: +1715 to +2788.
Genotype Determination
ES Cell Line
AK7 derived from 129S4/SvJaeSor
Phenotype
Homozygous: Postnatal lethal due to neonatal diabetes Pancreas--5% insulin production, no islet formation Gut--no CCK or secretin production Inner ear defects resulting in deafness--no cochlear ganglia and poor vestibular ganglia formation Cerebellar defect resulting in ataxia-- >90% depletion of cerebellar granule cells Hippocampal defects--no dentate gyrus
Mammalian Phenotype Terms
Allelic Composition: Neurod1tm1Jle/Neurod1tm1Jle (Genetic Background: involves: 129S4/SvJaeSor * C57BL/6 )

Allelic Composition: Neurod1tm1Jle/Neurod1tm1Jle (Genetic Background: involves: 129S4/SvJaeSor * ICR )

MeSH Terms
  • Animals
  • Apoptosis
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Count
  • Cell Differentiation
  • Cerebellum/cytology
  • Cerebellum/growth & development
  • Cerebellum/pathology
  • Genetic Therapy
  • Hippocampus/cytology
  • Hippocampus/growth & development
  • Hippocampus/pathology
  • In Situ Nick-End Labeling
  • Insulin/genetics
  • Mice
  • Mice, Knockout
  • Mice, Neurologic Mutants
  • Mice, Transgenic
  • Nerve Tissue Proteins/deficiency
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Neurons/cytology
  • Phenotype
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins/physiology
  • Transgenes
  • Cell Death
  • Cell Movement
  • Cell Survival
  • Cochlea/growth & development
  • Cochlea/innervation
  • Cochlea/pathology
  • Cochlea/ultrastructure
  • Deafness/genetics
  • Deafness/physiopathology
  • Evoked Potentials, Auditory/genetics
  • Evoked Potentials, Auditory/physiology
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Hair Cells, Auditory, Inner/growth & development
  • Hair Cells, Auditory, Inner/metabolism
  • Hair Cells, Auditory, Inner/pathology
  • Hair Cells, Auditory, Inner/ultrastructure
  • Helix-Loop-Helix Motifs
  • Histocytochemistry
  • In Situ Hybridization
  • Microscopy, Electron
  • Nerve Tissue Proteins/chemistry
  • Neural Pathways/growth & development
  • Neural Pathways/pathology
  • Receptor, trkB/genetics
  • Receptor, trkB/metabolism
  • Receptor, trkC/genetics
  • Receptor, trkC/metabolism
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Jackie Lee, Ph.D., University of Colorado at Boulder
Primary Reference
  • Miyata T, Maeda T, Lee JE. NeuroD is required for differentiation of the granule cells in the cerebellum and hippocampus. Genes Dev. 1999 Jul 1;13(13):1647-52. (Medline PMID: 10398678)
  • Kim WY, Fritzsch B, Serls A, Bakel LA, Huang EJ, Reichardt LF, Barth DS, Lee JE. NeuroD-null mice are deaf due to a severe loss of the inner ear sensory neurons during development. Development. 2001 Feb;128(3):417-26. (Medline PMID: 11152640)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
agouti
MMRRC Breeding System
Intrastrain
Breeding Scheme(s)
  • Heterozygous female x Wild type male
Generation
Unknown
Overall Breeding Performance
Normal
Average litter size
6 - 8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000367-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.