Strain Name:
B6.129X1(Cg)-Atp4atm1Ges/Mmjax
Stock Number:
036712-JAX
Citation ID:
RRID:MMRRC_036712-JAX
Other Names:
Atp4a, gastric H,K-ATPase

Gene Information

Atp4atm1Ges
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide; targeted mutation 1, Gary E Shull
Synonyms: H/Kalpha-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Genetic Alterations
The mutant allele of Atp4a was generated in 129/SvJ ES cells by replacing codons 360–390 in exon 8 with the neomycin resistance gene. The region that was deleted encodes sequences extending from the fourth transmembrane domain to just beyond the conserved phosphorylation site (Asp385), which is required for enzyme activity.
Genotype Determination
ES Cell Line
Not specified
Phenotype

Homozygous: Because this gene encodes the catalytic subunit of the gastric H,K-ATPase (the gastric proton pump) the mice lack acid secretion in the stomach; like all achlorhydric mice as they age they develop gastric metaplasia. Null mutants appear completely normal and both male and female are fertile.

Heterozygous: Normal

Mammalian Phenotype Terms
Allelic Composition: Atp4atm1Ges/Atp4atm1Ges (Genetic Background: involves: 129X1/SvJ * Black Swiss )

Allelic Composition: Atp4am1Btlr/Atp4atm1Ges (Genetic Background: involves: 129X1/SvJ * Black Swiss * C57BL/6J )

MeSH Terms
  • Achlorhydria/blood
  • Achlorhydria/genetics
  • Achlorhydria/pathology
  • Animals
  • Cilia/pathology
  • Electrolytes/blood
  • Gastric Acid/secretion
  • Gastric Mucosa/pathology
  • Gastric Mucosa/ultrastructure
  • H(+)-K(+)-Exchanging ATPase/deficiency
  • H(+)-K(+)-Exchanging ATPase/genetics
  • H(+)-K(+)-Exchanging ATPase/metabolism
  • Metaplasia
  • Mice
  • Mice, Knockout
  • Parietal Cells, Gastric/pathology
  • Parietal Cells, Gastric/ultrastructure
  • Pepsinogen A/analysis
  • Genotype
  • Intracellular Membranes/enzymology
  • Intracellular Membranes/ultrastructure
  • Microscopy, Electron
  • Microvilli/enzymology
  • Microvilli/ultrastructure
  • Parietal Cells, Gastric/enzymology
  • Protein Subunits
  • Secretory Vesicles/enzymology
  • Secretory Vesicles/ultrastructure
  • Sodium-Hydrogen Exchangers/genetics
  • Sodium-Hydrogen Exchangers/metabolism
  • Acids/metabolism
  • Amiloride/analogs & derivatives
  • Amiloride/pharmacology
  • Anti-Arrhythmia Agents/pharmacology
  • Diet
  • Female
  • Hydrogen-Ion Concentration/drug effects
  • Kidney Tubules, Collecting/cytology
  • Kidney Tubules, Collecting/metabolism
  • Kidney Tubules, Collecting/secretion
  • Macrolides/pharmacology
  • Mice, Inbred C57BL
  • Protein Isoforms/metabolism
  • Proton-Translocating ATPases/antagonists & inhibitors
  • Protons
Strain Development
Chimeric mice were generated by blastocyst-mediated transgenesis and mated to Black Swiss mice. Offspring with ES cell-derived genetic material were identified by their agouti coat color and confirmed with Southern blot analysis. The phenotype (loss of gastric acid secretion) was determined on a mixed 129/SvJ and Black Swiss background. The strain was then backcrossed to C57BL/6 for more than 10 generations, on which the phenotype remains the same.
Suggested Control Mice
Wild-type littermates or C57BL/6
  • Apoptosis
  • Cancer
  • Cell Biology
  • Endocrine Deficiency
  • Immunology and Inflammation
  • Metabolism
Donor
Gary Shull, Ph.D., University of Cincinnati College of Medicine.
Primary Reference

Spicer Z, Miller ML, Andringa A, Riddle TM, Duffy JJ, Doetschman T, Shull GE. Stomachs of mice lacking the gastric H,K-ATPase alpha -subunit have achlorhydria, abnormal parietal cells, and ciliated metaplasia. J Biol Chem. 2000 Jul 14;275(28):21555-65. (Medline PMID:10764766).

Miller ML, Judd LM, Van Driel IR, Andringa A, Flagella M, Bell SM, Schultheis PJ, Spicer Z, Shull GE. The unique ultrastructure of secretory membranes in gastric parietal cells depends upon the presence of H+, K+ -ATPase. Cell Tissue Res. 2002 Sep;309(3):369-80. (Medline PMID:12195293).

Lynch IJ, Rudin A, Xia SL, Stow LR, Shull GE, Weiner ID, Cain BD, Wingo CS. Impaired acid secretion in cortical collecting duct intercalated cells from H-K-ATPase-deficient mice: role of HKalpha isoforms. Am J Physiol Renal Physiol. 2008 Mar;294(3):F621-7. (Medline PMID:18057185).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)

C57BL/6 female x Heterozygous male or reciprocal mating

Wild-type female x Heterozygous male littermate or reciprocal mating.

Homozgyous female x Homozygous male littermate

Generation
N>10 generations
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 month Unknown
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036712-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
036712-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.