Strain Name:
STOCK Atp2b1tm1Ges/Mmjax
Stock Number:
036714-JAX
Citation ID:
RRID:MMRRC_036714-JAX
Other Names:
Atp2b1, PMCA1, plasma membrane Ca-ATPase isoform 1

Strain Information

Atp2b1tm1Ges
Name: ATPase, Ca++ transporting, plasma membrane 1; targeted mutation 1, Gary E Shull
Synonyms: Pmca1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Atp2b1
Name: ATPase, Ca++ transporting, plasma membrane 1
Synonyms: PMCA1, 2810442I22Rik, E130111D10Rik
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 67972
VEGA: 10
HGNC: HGNC:814
Homologene: 55597
Genetic Alterations
Codons 466-529 in exon 10, including the catalytic phosphorylation site (aspartic acid 475), were replaced with a neomycin resistance gene. Northern blot indicated no difference in levels of mRNA between wild-type and heterozygous mice. RT-PCR confirmed that the exon 9 was spliced to exon 11, producing the transcript of the expected length.
ES Cell Line
Not specified
Phenotype

Homozygous: Embryonic lethality during preimplantation stage.

Heterozygous: Increased smooth muscle cell apoptosis under certain conditions; increased force in bladder smooth muscle

Mammalian Phenotype Terms
Allelic Composition: Atp2b1tm1Ges/Atp2b1tm1Ges (Genetic Background: involves: 129X1/SvJ * Black Swiss )

MeSH Terms
  • Alleles
  • Animals
  • Apoptosis
  • Binding Sites/genetics
  • Blotting, Northern
  • Calcium-Transporting ATPases/analysis
  • Calcium-Transporting ATPases/genetics
  • Calcium-Transporting ATPases/physiology
  • Cation Transport Proteins
  • Fertility/physiology
  • Heterozygote
  • Immunoblotting
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Knockout
  • Microscopy, Electron
  • Muscle Contraction
  • Muscle, Smooth, Vascular/cytology
  • Muscle, Smooth, Vascular/physiology
  • Mutagenesis
  • Phenotype
  • Phosphorylation
  • Plasma Membrane Calcium-Transporting ATPases
  • Portal Vein/cytology
  • Portal Vein/physiology
  • RNA, Messenger/analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sperm Motility/physiology
  • Sperm Tail/chemistry
  • Spermatozoa/chemistry
  • Spermatozoa/ultrastructure
  • Calcium/metabolism
  • Calcium-Transporting ATPases/antagonists & inhibitors
  • Calcium-Transporting ATPases/metabolism
  • Carbachol/metabolism
  • Cation Transport Proteins/antagonists & inhibitors
  • Cation Transport Proteins/genetics
  • Cation Transport Proteins/metabolism
  • Cell Membrane/enzymology
  • Cholinergic Agonists/metabolism
  • Homeodomain Proteins
  • Muscle Contraction/physiology
  • Muscle, Smooth/cytology
  • Muscle, Smooth/physiology
  • Potassium Chloride/metabolism
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Sodium-Calcium Exchanger/antagonists & inhibitors
  • Sodium-Calcium Exchanger/genetics
  • Sodium-Calcium Exchanger/metabolism
  • Urinary Bladder/anatomy & histology
  • Urinary Bladder/physiology
  • Carbachol/administration & dosage
  • Carbachol/pharmacology
  • Dose-Response Relationship, Drug
  • Homeostasis
  • Intracellular Membranes/metabolism
  • Isoenzymes/metabolism
  • Kinetics
  • Muscle Contraction/drug effects
  • Muscle, Smooth/enzymology
  • Muscle, Smooth/metabolism
  • Osmolar Concentration
  • Plasma Membrane Calcium-Transporting ATPases/deficiency
  • Plasma Membrane Calcium-Transporting ATPases/metabolism
  • Potassium Chloride/pharmacology
  • Receptors, Drug/metabolism
  • Signal Transduction/physiology
  • Urinary Bladder/enzymology
  • Urinary Bladder/metabolism
Strain Development
The initial chimeras were bred with Black Swiss mice and have been kept on the mixed background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Internal Organ
Donor
Gary Shull, Ph.D., University of Cincinnati College of Medicine.
Primary Reference

Okunade GW, Miller ML, Pyne GJ, Sutliff RL, O'Connor KT, Neumann JC, Andringa A, Miller DA, Prasad V, Doetschman T, Paul RJ, Shull GE. Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem. 2004 Aug 6;279(32):33742-50. (Medline PMID:15178683).

Liu L, Ishida Y, Okunade G, Shull GE, Paul RJ. Role of plasma membrane Ca2+-ATPase in contraction-relaxation processes of the bladder: evidence from PMCA gene-ablated mice. Am J Physiol Cell Physiol. 2006 Apr;290(4):C1239-47. (Medline PMID:16291816).

Liu L, Ishida Y, Okunade G, Pyne-Geithman GJ, Shull GE, Paul RJ. Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Am J Physiol Cell Physiol. 2007 Jan;292(1):C423-31. (Medline PMID:16956963).

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Agouti, with occasional black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Wild-type female x Heterozygous male or reciprocal mating

Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 8 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036714-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
036714-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.