Strain Name:
STOCK Tg(Hoxb7-rtTA*M2)RS40BCos/Mmjax
Stock Number:
036718-JAX
Citation ID:
RRID:MMRRC_036718-JAX
Other Names:
Hoxb7/rtTA, RS40B

Gene Information

Hoxb7
Name: homeobox B7
Synonyms: Hox-2.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15415
HGNC: HGNC:5118
Homologene: 55828
Tg(Hoxb7-rtTA*M2)RS40BCos
Name: transgene insertion RS40B, Frank Costantini
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The Hoxb7-rtTA*M2 transgene was designed with the homeobox B7 (Hoxb7) promoter directing expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein, followed by a simian virus 40 (SV40) polyadenylation (polyA) signal.  multiple copies of the transgene are inserted at an unknown chromosomal location.
Genotype Determination
Phenotype

Homozygous: Not evaluated

Hemizygous: The transgene itself causes no dominant phenotype. However, it can be crossed to strains carrying a Tet-operator-driven gene, and used for Tet-regulated expression of that other gene in the Hoxb7 expression domain. For example, it has been used to mis-express the secreted protein GDNF in abnormal sites during kidney development.

MeSH Terms
  • Animals
  • Body Patterning
  • Gene Expression Regulation, Developmental
  • Glial Cell Line-Derived Neurotrophic Factor
  • Homeodomain Proteins/genetics
  • In Situ Hybridization
  • Kidney/embryology
  • Mesoderm/cytology
  • Mesoderm/physiology
  • Mice
  • Mice, Transgenic
  • Nerve Growth Factors/genetics
  • Nerve Growth Factors/physiology
  • Restriction Mapping
  • Ureter/abnormalities
  • Ureter/embryology
Strain Development
This is a transgenic line in which the reverse tetracycline transactivator rtTA-2SM2 is driven by the Hoxb7 promoter. It was backcrossed to C57BL/6J for nine generations, then crossed to 129/SvEv for one or two generations, before males were used for sperm cryopreservation. We did this because we noticed some silencing of the transgene, which we believed was due to the B6 background.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Developmental Biology
  • Internal Organ
  • Research Tools
Donor
Frank Costantini, Ph.D., Columbia University Medical Center.
Primary Reference

Shakya R, Jho EH, Kotka P, Wu Z, Kholodilov N, Burke R, D'Agati V, Costantini F. The role of GDNF in patterning the excretory system. Dev Biol. 2005 Jul 1;283(1):70-84. (Medline PMID: 15890330).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti or black
MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate.  Donor recommends outcrossing because some silencing of the transgene was observed while backcrossing to C57BL/6J. This is believed to be due to the B6 background.
Breeding Scheme(s)

Outbred or Hybrid female x Hemizygous male or reciprocal mating

Hemizygous female x Hemizygous male

Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Unknown
Recommended wean age
Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036718-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036718-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.