Strain Name:
C.129X1(B6)-Nkx1-2tm1Lmgd/Mmnc
Stock Number:
036727-UNC
Citation ID:
RRID:MMRRC_036727-UNC
Other Names:
Nkx-1.2-/+,

Strain Information

Nkx1-2tm1Lmgd
Name: NK1 homeobox 2; targeted mutation 1, Heiner Westphal
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Nkx1-2
Name: NK1 homeobox 2
Synonyms: Nkx-1.1, Sax1, Nkx-1.2
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 20231
Homologene: 7525
Genetic Alterations
A neo cassette was introduced that replaces the entire coding region of the gene (exons 1 and 2).
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype

    Homozygous: Mice show an altered performance in the force-swim test. Possibly also altered behavior in the Morris-maze test.

    Heterozygous: Not provided.

    Strain Development
    Original strain was created in by injecting 129X1/SvJ ES cells bearing a Nkx1.2 null allele (neomycin cassette insertion into coding region) into C57BL/6 embryos. Heterozygous progeny from the mixed B6/129 background were backcrossed into BalB/cJ.
    Suggested Control Mice
    Wild-type littermates or BALB/cJ
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Developmental Biology
    • Metabolism
    • Neurobiology
    Donor
    Alessandra Rovescalli, Ph.D., National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health.

    Colony and Husbandry Information

    Breed mice while young, females older than 3 months often experience dystocia.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    White
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)

    BALB/cJ female x Heterozygous male (2nd litters may be more successful breeding to BALB/c females)
    Heterozygous female x BALB/cJ male

    Generation
    N20
    Overall Breeding Performance
    Good.  Age of female is of the essence to ensure success breeding. Females older than 3 months often undergo dystocia.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 months Unknown
    Average litter size
    5-9
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    036727-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.