Strain Name:
FVB.Cg-Tg(tetO-GLI1)10Rup/Mmjax
Stock Number:
036729-JAX
Citation ID:
RRID:MMRRC_036729-JAX
Other Names:
TRE-GLi1

Gene Information

GLI1
Name: GLI family zinc finger 1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 12
tetO
Name: tetracycline response element
Type: Regulatory Region
Species: Multi-species
Tg(tetO-GLI1)10Rup
Name: transgene insertion 10, J Ruppert
Synonyms: TRE-GLi1
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
A 5.7-kb transgenic construct containing a tetracycline-responsive element (TRE or tetO), the minimal human cytomegalovirus promoter, and an HA-tagged, wild-type, human GLI1, GLI1-Kruppel family member GLI1 was injected into fertilized (C57BL/6 x SJL)F2 mouse eggs.
Phenotype

These transgenic mice express human GLI1 (GLI family zinc finger 1) under the direction of the tetracycline operator promoter (tetO). Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. It is not known if homozygotes are viable. When mated to mice carrying the Tg(KRT14-rtTA)208Jek allele (MGI:3717751, also known as "K14-rtTA"), GLI1 expression is directed to skin cells and regulated with tetracycline (or its analog doxycycline, DOX). Following DOX induction, bitransgenic mice exhibit dorsal hair loss and skin lesions characterized by nests of basaloid cells budding from the epidermis. This strain may be useful for studying cutaneous basal cell carcinoma (BCC).

MeSH Terms
  • Animals
  • Base Sequence
  • Carcinoma, Basal Cell/etiology
  • Cell Cycle
  • Cell Transformation, Neoplastic
  • Hyperplasia
  • Kruppel-Like Transcription Factors/physiology
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • RNA, Small Interfering/pharmacology
  • Skin/pathology
  • Skin Neoplasms/etiology
  • Snail Family Transcription Factors
  • Transcription Factors/genetics
  • Transcription Factors/physiology
  • Zinc Finger Protein GLI1
Strain Development
The transgene was injected into fertilized (C57BL/6 x SJL)F2 mouse eggs. Founder line 10 was subsequently established. Founders were bred to (C57BL/6 x SJL)F2 mice and then to FVB/NJ for at least 8 generations. Upon arrival the mice were bred with FVB/NJ mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates or FVB/NJ
Donor
Michael Ruppert, M.D., West Virginia University.
Primary Reference

Li X, Deng W, Nail CD, Bailey SK, Kraus MH, Ruppert JM, Lobo-Ruppert SM. Snail induction is an early response to Gli1 that determines the efficiency of epithelial transformation. Oncogene. 2006 Jan 26;25(4):609-21. (Medline PMID: 16158046).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
White
MMRRC Breeding System
Backcross
Breeding Scheme(s)

FVB/NJ females x Hemizygous male or reciprocal mating

Generation
N9+ (FVB/NJ)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036729-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
036729-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.