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Strain Detail Sheet

Strain Name:
FVB.Cg-Myh9tm1.1Gac Tg(NPHS2-cre)295Lbh/Mmjax
Stock Number:
036742-JAX
Citation ID:
RRID:MMRRC_036742-JAX
Other Names:
Podocin::Cre/+; Myh9flox/flox, Podocyte KO

Strain Information

Gene Details

Myh9tm1.1Gac
Name: myosin, heavy polypeptide 9, non-muscle; targeted mutation 1.1, Christian Gachet
Synonyms: Myh9flox, MYH9Flx
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Conditional
Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Conditional
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Alteration at locus: Conditional
NPHS2
Name: NPHS2 stomatin family member, podocin
Type: Gene
Species: Homo sapiens (human)
Chromosome: 1
Alteration at locus: Conditional
Tg(NPHS2-cre)295Lbh
Name: transgene insertion 295, Lawrence B Holzman
Synonyms: pod-Cre, PodoCre, 2.5P-Cre, NPHS2.Cre, podocin-Cre, Tg(NPHS2-cre)1Lbh
Type: Transgene
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
LoxP sites inserted by C. Leon surrounding what she termed Myh9 exon 1, which is actually exon 2 per Ensembl. The Cre allele is still present.
Genotype Determination
ES Cell Line
Myh9 allele: Non-specified ESC line derived from 129/Sv

Strain Description

Phenotype

Homozygous: Separately, the Podocin::Cre and Myh9 flox alleles have no phenotype. Together, a Podocin::Cre/+; Myh9flox/flox mouse is predisposed to glomerular diseaes from 2nd hits. With CMV-Cre or ubiquitin-ERt2 inducible Cre, deletion of the Myh9 flox allele proceeds efficiently and is lethal.

Heterozygous: Undetermined.

MeSH Terms
  • Animals
  • Apolipoprotein L1
  • Apolipoproteins/genetics
  • Cell Line
  • Doxorubicin/administration & dosage
  • Genetic Predisposition to Disease
  • Glomerulosclerosis, Focal Segmental/genetics
  • Humans
  • Kidney Diseases/chemically induced
  • Kidney Diseases/genetics
  • Lipoproteins, HDL/genetics
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Myosin Heavy Chains/genetics
  • Myosin Heavy Chains/physiology
  • Nonmuscle Myosin Type IIA/genetics
  • Nonmuscle Myosin Type IIA/physiology
  • Nonmuscle Myosin Type IIB/genetics
  • Nonmuscle Myosin Type IIB/physiology
  • Podocytes
  • Proteinuria/genetics
  • Sequence Deletion
  • Bleeding Time
  • Blood Platelets/metabolism
  • Blood Platelets/pathology
  • Female
  • Hemostasis/genetics
  • Male
  • Megakaryocytes/metabolism
  • Mice, Transgenic
  • Myosin Heavy Chains/metabolism
  • Nonmuscle Myosin Type IIA/deficiency
  • Nonmuscle Myosin Type IIA/metabolism
  • Organ Specificity/genetics
  • Platelet Aggregation/genetics
  • Thrombocytopenia/blood
  • Thrombocytopenia/genetics
  • Thrombocytopenia/pathology
Strain Development
The Podocyte::Cre transgene has been previous described and submitted to JAX on the C57BL/6 background by Dr. L Holzman. The Myh9 flox allele was created by C. Leon and C. Gachet (Strasbourg), then transferred to Drs. L. Holzman and D. Johnstone at the University of Michigan. The Myh9 floxed allele was additionally backcrossed five times to C57BL/6 by the donor. The Pod::Cre and Myh9 flox alleles were then backcrossed to FVB/N at the University of Pennsylvania, first using speed congenics until the 4th generation, then classical backcrossing to FVB/N until N10. FVB/N stocks were bred from FVB/NJ obtained through JAX. At each generation, double mutant mice were selected by PCR genotyping. Based on genotyping, the insertion site of the Pod::Cre transgene is not linked to the Myh9 locus.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Hematology
  • Immunology and Inflammation
  • Research Tools
  • Sensorineural

Strain Origin

Donor
Duncan Johnstone, M.D., University of Pennsylvania: Medicine.
Lawrence B. Holzman, M.D., University of Pennsylvania.
Christian Gachet, M.D., INSERM Institut National de la Sante et de la Recherche Medicale.
Primary Reference

Leon C, Eckly A, Hechler B, Aleil B, Freund M, Ravanat C, Jourdain M, Nonne C, Weber J, Tiedt R, Gratacap MP, Severin S, Cazenave JP, Lanza F, Skoda R, Gachet C. Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion. Blood. 2007 Nov 1;110(9):3183-91. Epub 2007 Jul 30. (Medline PMID: 17664350).

Johnstone DB, Zhang J, George B, Leon C, Gachet C, Wong H, Parekh R, Holzman LB. Podocyte-specific deletion of Myh9 encoding nonmuscle myosin heavy chain 2A predisposes mice to glomerulopathy. Mol Cell Biol. 2011 May;31(10):2162-70. Epub 2011 Mar 14. (Medline PMID: 21402784).

Colony and Husbandry Information

Special Considerations

An occassional and unpredictable occuranace of litter canabalism by parents, including after fathers were removed in timed matings and mothers were siblings and had shared a cage throughout. May be sensitive to handling stress.

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
White
Eye
Red

Breeding

MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • For each generation, a Podocin::Cre/+
  • Myh9flox/+ double heterozygote is mated either to pure FVB/N (to backcross), or to Myh9flox/flox (for an experimental cross of the KO animal and control littermates).
Generation
N10 (FVB/NJ)
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 12 months
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
036742-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
036742-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / $2,123.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.