Strain Name:
B6.129-Ctnnd2tm1Lxin/Mmucd
Stock Number:
036797-UCD
Citation ID:
RRID:MMRRC_036797-UCD
Other Names:
delta-catenin mutant

Strain Information

Ctnnd2tm1Lxin
Name: catenin delta 2; targeted mutation 1, Xin Liu
Synonyms: delta-cat-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Knock-In
Ctnnd2
Name: catenin delta 2
Synonyms: catenin (cadherin associated protein), delta 2, neurojugin, Catnd2, Nprap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Knock-In
NCBI: 18163
VEGA: 15
HGNC: HGNC:2516
Homologene: 55574
Genetic Alterations
GFP followed by a stop codon and a PGK-hygro cassette were inserted within exon 9 of the Ctnnd2 locus, preventing transcription of the carboxyl terminal region.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype

Homozygous: many synaptic and behavioral deficits

Heterozygous: none identified

Mammalian Phenotype Terms
Allelic Composition: Ctnnd2tm1Lxin/Ctnnd2tm1Lxin (Genetic Background: involves: 129S4/SvJae * C57BL/6NTac )

MeSH Terms
  • Aging
  • Animals
  • Catenins
  • Cell Adhesion Molecules/genetics
  • Cell Adhesion Molecules/metabolism
  • Cell Size
  • Cerebral Cortex/cytology
  • Cerebral Cortex/growth & development
  • Cerebral Cortex/physiology
  • Dendrites/physiology
  • Dendritic Spines/physiology
  • Electric Stimulation
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Kinetics
  • Mice
  • Mice, Transgenic
  • Microelectrodes
  • Mutation
  • Neurons/cytology
  • Neurons/physiology
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
  • Pyramidal Cells/cytology
  • Pyramidal Cells/physiology
  • Visual Cortex/cytology
  • Visual Cortex/growth & development
  • Visual Cortex/physiology
  • Analysis of Variance
  • Armadillo Domain Proteins
  • Cadherins/metabolism
  • Cell Adhesion Molecules
  • Cognition Disorders/etiology
  • Conditioning, Psychological/physiology
  • Cytoskeletal Proteins/deficiency
  • Cytoskeletal Proteins/genetics
  • Disks Large Homolog 4 Protein
  • Electrophysiology
  • Fear/physiology
  • Guanylate Kinases
  • Hippocampus/physiopathology
  • Immunoprecipitation
  • Intracellular Signaling Peptides and Proteins
  • Maze Learning/physiology
  • Membrane Proteins
  • Mice, Mutant Strains
  • Mutation/genetics
  • Nerve Tissue Proteins/metabolism
  • Neurodegenerative Diseases/etiology
  • Neuronal Plasticity/physiology
  • Neurons/metabolism
  • Phosphoproteins
  • Psychomotor Performance/physiology
  • Synapses/genetics
  • Synapses/metabolism
  • Delta Catenin
Strain Development
Linearized target construct was electroporated into 129S4/SvJ ES cells. The selected ES clones were microinjected into C57BL/6NTac blastocysts and the F1 progeny was crossed with C57BL/6NTac. The heterozygous littermates were mated with C57BL/6NTac for 6 generations.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology
Donor
Xin Liu, Ph.D., David Geffen School of Medicine at University of California, Los Angeles.
Primary Reference
  • Israely I, Costa RM, Xie CW, Silva AJ, Kosik KS, Liu X. Deletion of the neuron-specific protein delta-catenin leads to severe cognitive and synaptic dysfunction. Curr Biol. 2004 Sep 21;14(18):1657-63. (Medline PMID 15380068).
  • Matter C, Pribadi M, Liu X, Trachtenberg JT. Delta-catenin is required for the maintenance of neural structure and function in mature cortex in vivo. Neuron. 2009 Nov 12;64(3):320-7. doi: 10.1016/j.neuron.2009.09.026 (Medline PMID: 19914181).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Homozygous female x Homozygous male littermate
Generation
N6
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Not specified
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036797-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036797-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036797-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.