Strain Name:
STOCK Mir325tm1Mtm/Mmjax
Stock Number:
036800-JAX
Citation ID:
RRID:MMRRC_036800-JAX
Major Collection:

Gene Information

Mir325tm1Mtm
Name: microRNA 325; targeted mutation 1, Michael McManus
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Conditional
Mir325
Name: microRNA 325
Synonyms: mir 325, mmu-mir-325, Mirn325
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Conditional
NCBI: 723929
VEGA: X
Genetic Alterations
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the miR129-1 stem loop, with one loxP site placed immediately downstream of the miR129-1 stem loop.
Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Mammalian Phenotype Terms
Allelic Composition: Mir325tm1Mtm/Mir325tm1Mtm (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

  • phenotype not analyzed
MeSH Terms
  • Animals
  • Embryonic Stem Cells
  • Gene Expression Profiling
  • Genes, Reporter
  • Genotype
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • MicroRNAs/genetics
  • MicroRNAs/metabolism
  • MicroRNAs/physiology
  • Models, Animal
  • Models, Genetic
Donor
Michael McManus, Ph.D., University of California, San Francisco.
Primary Reference
Park CY, Jeker LT, Carver-Moore K, Oh A, Liu HJ, Cameron R, Richards H, Li Z, Adler D, Yoshinaga Y, Martinez M, Nefadov M, Abbas AK, Weiss A, Lanier LL, de Jong PJ, Bluestone JA, Srivastava D, McManus MT. A Resource for the Conditional Ablation of microRNAs in the Mouse. Cell Rep. 2012 Apr 19;1(4):385-91. (Medline PMID: 22570807).

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036800-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036800-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.