Strain Name:
B6.Cg-Tg(CMV-tTA,tetO-Stxbp3)40Jeps/Mmmh
Stock Number:
036820-MU
Citation ID:
RRID:MMRRC_036820-MU
Other Names:

Tg(Munc18c), Tg(Munc18c)-40 and Tg(Munc18c)-31, B6.Cg-Tg(CMV-tTA,tetO-Stxbp3)40Jepn/Mmmh

Strain Information

Stxbp3
Name: syntaxin binding protein 3
Synonyms: Munc-18c, Stxbp3, Stxbp3a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20912
Homologene: 5260
CMV
Name: cytomegalovirus
Type: Regulatory Region
Species: Virus
Chromosome:
Tg(CMV-tTA,tetO-Stxbp3)40Jpes
Name: transgene insertion 40, Jeffrey E Pessin
Synonyms: Tg(CMV-tTA,tetO-Stxbp3a)40Jepn, Munc18c Tg
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations
A transgenic construct contains mouse Stxbp3a (Munc18c) gene under the control of the tetracycline repressive CMV promoter.
Genotype Determination
Phenotype

Homozygous: Transgene has not yet been bred to homozygosity.

Hemizygous:  The heterozygote mice are phenotypically normal overall. These mice over-express Munc18c 4-5 fold over that of endogenous simultaneously in at least 3 major tissues: adipose, skeletal muscle and pancreas. The transgene is tetracycline repressible. In the absence of tetracycline, the mice are insulin resistant and glucose intolerant, with known defects in insulin secretion and insulin-stimulated glucose clearance by skeletal muscle.

MeSH Terms
  • Adipose Tissue/metabolism
  • Animals
  • Anti-Bacterial Agents
  • Gene Expression/drug effects
  • Glucose/pharmacokinetics
  • Glucose Intolerance/metabolism
  • Glucose Intolerance/physiopathology
  • Homeostasis/physiology
  • Insulin/secretion
  • Insulin Resistance/physiology
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Transgenic
  • Munc18 Proteins
  • Muscle, Skeletal/metabolism
  • Nerve Tissue Proteins
  • Proteins/genetics
  • Proteins/metabolism
  • Qa-SNARE Proteins
  • Tetracycline
  • Vesicular Transport Proteins
Strain Development
Munc18c gene (from Mus musculus) was inserted into the plasmid (pUC-COMBIcmv) to generate tetracycline-repressible transgenic mice. The transgenic constrct was microinjected into the nucleus of preimplantation embryos and transferred into the oviduct of pseudo-pregnant C57BL/6J female mice. Pups were screened for the presence of the transgene using PCR of genomic DNA and positive animals were bred with C57BL/6J.
Suggested Control Mice
Wild-type littermates or C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Diabetes
  • Metabolism
  • Obesity
Donor
Jeffrey E Pessin, Ph.D., Albert Einstein College of Medicine.
Debbie Thurmond, Ph.D., Indiana University School of Medicine.
Primary Reference

Spurlin BA, Thomas RM, Nevins AK, Kim HJ, Kim YJ, Noh HL, Shulman GI, Kim JK, Thurmond DC. Insulin resistance in tetracycline-repressible Munc18c transgenic mice. Diabetes. 2003 Aug;52(8):1910-7. (Medline PMID: 12882905).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
C57BL/6J female x Hemizygous male or reciprocal mating
Generation
N20+ (C57BL/6J)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: N/A N/A
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 10 months
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036820-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036820-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.