Strain Name    : B6.FVB-Tg(tetO-Ccnd1*T286A,-luc)11233Kuw/Mmmh

Stock Number : 036906-MU

Other Names   : TetO-Cyclin D1

Gene Information

(provided by MGI)
Transgene: Tg(tetO-Ccnd1*T286A,-luc)11233Kuw
Name: transgene insertion 11233, Kay-Uwe Wagner
Alteration at locus: Transgenic (random, gene disruption)
Promoter: tetO (Synthetic)
Name: tetracycline operon
Alteration at locus: Transgenic (random, gene disruption)
Transgene: Ccnd1*T286A
Name:
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations:
The tetracycline operator drives inducible expression of the FLAG-tagged mouse Ccnd1 cDNA with a point mutation that results in the amino acid substitution of alanine for threonine at position 286 (T286A) and an IRES-luciferase.

Genotype Determination:

ES Cell Line: Not Applicable

Phenotype

Homozygous and Hemizygous mice do not have a phenotype.

Strain of Origin: FVB/N

Strain genetic background: B6.FVB/N

Strain Development: The targeted construct vector was microinjected into pronucleus of fertilized mouse eggs of FVB/N mice and offspring of founders were mated to FVB/N mice. The transgenic mice were backcrossed with C57BL/6 mice for 10 generations.
MMRRC currently maintains MMRRC:036897, FVB/N-Tg(tetO-Ccnd1*T286A,-luc)11233Kuw/Mmmh.   

Suggested Control Mice: Wild-type littermates or C57BL/6 mice

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
    • breast cancer
    • lymphoma
  • Research Tools

Donor: Kay-Uwe Wagner, Ph.D., University of Nebraska Medical Center.

Primary Reference:

Zhang Q, Sakamoto K, Liu C, Triplett AA, Lin WC, Rui H, Wagner KU. Cyclin D3 Compensates for the Loss of Cyclin D1 during ErbB2-Induced Mammary Tumor Initiation and Progression. Cancer Res 2011 71 (24) 7513-24 (Medline PMID: 22037875)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Black

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating.

Generation: N10

Overall Breeding Performance: Excellent

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined

Average litter size: 5-9

Recommended wean age: 3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
036906-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
036906-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.