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Strain Name:
B6.FVB-Tg(tetO-Ccnd1*T286A,-luc)11233Kuw/Mmmh
Stock Number:
036906-MU
Citation ID:
RRID:MMRRC_036906-MU
Other Names:
TetO-Cyclin D1

Strain Information

Ccnd1
Name: cyclin D1
Synonyms: Cyl-1, bcl-1, PRAD1, cD1, CycD1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12443
HGNC: HGNC:1582
Homologene: 1334
tetOp
Name: tetracycline operon
Type: Gene
Species: Synthetic
Chromosome:
Tg(tetO-Ccnd1*T286A,-luc)11233Kuw
Name: transgene insertion 11233, Kay-Uwe Wagner
Synonyms: TetO-D1
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations
The tetracycline operator drives inducible expression of the FLAG-tagged mouse Ccnd1 cDNA with a point mutation that results in the amino acid substitution of alanine for threonine at position 286 (T286A) and an IRES-luciferase.
Genotype Determination
Phenotype

Homozygous: mice do not have a phenotype.

Hemizygous: mice do not have a phenotype.

MeSH Terms
  • Animals
  • Blotting, Western
  • Breast Neoplasms/genetics
  • Breast Neoplasms/metabolism
  • Breast Neoplasms/pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cells, Cultured
  • Cyclin D1/genetics
  • Cyclin D1/metabolism
  • Cyclin D3/genetics
  • Cyclin D3/metabolism
  • Disease Progression
  • Female
  • Humans
  • Immunohistochemistry
  • Mammary Neoplasms, Animal/genetics
  • Mammary Neoplasms, Animal/metabolism
  • Mammary Neoplasms, Animal/pathology
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • NIH 3T3 Cells
  • RNA Interference
  • Receptor, ErbB-2/genetics
  • Receptor, ErbB-2/metabolism
Strain Development
The targeted construct vector was microinjected into pronucleus of fertilized mouse eggs of FVB/N mice and offspring of founders were mated to FVB/N mice. The transgenic mice were backcrossed with C57BL/6 mice for 10 generations.
MMRRC currently maintains MMRRC:036897, FVB/N-Tg(tetO-Ccnd1*T286A,-luc)11233Kuw/Mmmh.
Suggested Control Mice
Wild-type littermates or C57BL/6 mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
    • breast cancer
    • lymphoma
  • Research Tools
Donor
Kay-Uwe Wagner, Ph.D., University of Nebraska Medical Center.
Primary Reference

Zhang Q, Sakamoto K, Liu C, Triplett AA, Lin WC, Rui H, Wagner KU. Cyclin D3 Compensates for the Loss of Cyclin D1 during ErbB2-Induced Mammary Tumor Initiation and Progression. Cancer Res. 2011 Dec 15;71(24):7513-24. Epub 2011 Oct 28.(Medline PMID: 22037875)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating.

Generation
N10
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036906-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036906-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.