Strain Name:
B6.FVB(Cg)-Tg(Avp-EGFP)HF200Gsat/BmceMmucd
Stock Number:
036907-UCD
Citation ID:
RRID:MMRRC_036907-UCD
Other Names:
AVP-eGFP, argine vasopression, VP eGFP

Strain Information

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Avp
Name: arginine vasopressin
Synonyms: Vsp, Vp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11998
HGNC: HGNC:894
Homologene: 417
Tg(Avp-EGFP)HF200Gsat
Name: transgene insertion HF200, GENSAT Project at Rockefeller University
Synonyms: VP-eGFP
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
An EGFP reporter gene, followed by a polyadenylation sequence, was inserted into upstream of the ATG start codon of Avp (arginine vasopressin) in a selected BAC clone. This modification results in the expression of EGFP that is under the control of the promoter and regulatory elements of the Avp gene that is present on the BAC clone.
Phenotype

Homozygous: mice expresse EGFP in vasopression cells

Hemizygous: mice expresse EGFP in vasopression cells

MeSH Terms
  • Angiotensin II/administration & dosage
  • Angiotensin II/adverse effects
  • Animals
  • Cell Membrane/drug effects
  • Cell Membrane/metabolism
  • Cell Membrane/ultrastructure
  • Dendrites/metabolism
  • Dendrites/ultrastructure
  • Drug Administration Schedule
  • Drug Delivery Systems
  • Excitatory Amino Acid Agonists/pharmacology
  • Green Fluorescent Proteins/genetics
  • HEK293 Cells
  • Humans
  • Hypertension/chemically induced
  • Hypertension/pathology
  • In Vitro Techniques
  • Intracellular Membranes/metabolism
  • Intracellular Membranes/ultrastructure
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Immunoelectron
  • N-Methylaspartate/pharmacology
  • NADPH Oxidases/metabolism
  • Neuroglia/metabolism
  • Neuroglia/ultrastructure
  • Neurons/drug effects
  • Neurons/metabolism
  • Neurons/ultrastructure
  • Paraventricular Hypothalamic Nucleus/pathology
  • Protein Transport/drug effects
  • Reactive Oxygen Species/metabolism
  • Transfection
  • Vasoconstrictor Agents/administration & dosage
  • Vasoconstrictor Agents/adverse effects
  • Vasopressins/genetics
  • Vasopressins/metabolism
Strain Development
Modified BAC containing inserted EGFP into upstream of targeted gene was injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny were mated to Swiss Webster mice each generation. This transgenic mice were generated by GENSAT. Dr. McEwen backcrossed this strain to C57BL/6 for 10 generations. 
MMRRC currently maintains the STOCK strain MMRRC:015858, STOCK Tg(Avp-EGFP)HF200Gsat/Mmucd for this mutation.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Internal Organ
  • Neurobiology
  • Reproduction
  • Research Tools
Donor
Bruce McEwen, Ph.D., Rockefeller University.
Primary Reference

Coleman CG, Wang G, Faraco G, Marques Lopes J, Waters EM, Milner TA, Iadecola C, Pickel VM. Membrane Trafficking of NADPH Oxidase p47phox in Paraventricular Hypothalamic Neurons Parallels Local Free Radical Production in Angiotensin II Slow-Pressor Hypertension. J Neurosci. 2013 Mar 6;33(10):4308-16 (Medline PMID: 23467347).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating

Generation
N10
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
10-14
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036907-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036907-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036907-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.