Strain Name:
B6.FVB(Cg)-Tg(Drd2-EGFP)S118Gsat/KreMmucd
Stock Number:
036931-UCD
Citation ID:
RRID:MMRRC_036931-UCD
Other Names:
D2-GFP

Gene Information

Drd2
Name: dopamine receptor D2
Synonyms: D2R, Drd-2, D2 receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Transgenic (BAC)
NCBI: 13489
VEGA: 9
HGNC: HGNC:3023
Homologene: 22561
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Alteration at locus: Transgenic (BAC)
Tg(Drd2-EGFP)S118Gsat
Name: transgene insertion S118, GENSAT Project at Rockefeller University
Synonyms: Drd2-EGFP, D2GFP, BAC D2 EGFP, Founder Line S118, Tg(Drd2-EGFP)118Gsat
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
This BAC transgenic mouse expresses EGFP reporter gene which was inserted into the mouse BAC (RP23-161H15) at the ATG transcription initiation codon of the dopamine receptor 2 (Drd2) gene.
Genotype Determination
Phenotype

Homozygous: EGFP expression in Drd2-expressing cells

Hemizygous: EGFP expression in Drd2-expressing cells

MeSH Terms
  • Animals
  • Avoidance Learning/physiology
  • Behavior, Animal/physiology
  • Choice Behavior/physiology
  • Chromosomes, Artificial, Bacterial/genetics
  • Corpus Striatum/drug effects
  • Corpus Striatum/physiology
  • Dopamine Agents/pharmacology
  • Exploratory Behavior/physiology
  • Female
  • Hemizygote
  • Locomotion/genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic/genetics
  • Models, Animal
  • Receptors, Dopamine D1/genetics
  • Receptors, Dopamine D2/genetics
Strain Development
This was a BAC transgenic animal generated from GENSAT (Founder ID S118; BAC address RP23-161H15). Modified BAC containing inserted EGFP upstream of targeted gene injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny mated to Swiss Webster mice each generation. Dr. Kreitzer then backcrossed the strain to the C57BL/6 mouse for at least 10 generations.
Suggested Control Mice
Wild-type littermates
Donor
Anatol Kreitzer, Ph.D., The J. David Gladstone Institutes.
Primary Reference

Nelson AB, Hang GB, Grueter BA, Pascoli V, Luscher C, Malenka RC, Kreitzer AC. A comparison of striatal-dependent behaviors in wild-type and hemizygous Drd1a and Drd2 BAC transgenic mice. J Neurosci. 2012 Jul 4;32(27):9119-23. (Medline PMID: 22764221).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Wild-type female x Heterozygous male from the colony or reciprocal mating

Homozygous female x Homozygous male from the colony

Generation
N10+
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036931-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
036931-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
036931-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.