Allele Symbol: Lrsam1Gt(RRK461)Byg
Name: gene trap RRK461, BayGenomics
Alteration at locus: Gene Trap
Gene Symbol: Lrsam1
Name: leucine rich repeat and sterile alpha motif containing 1
Alteration at locus: Gene Trap
ES Cell Line: E14Tg2a.4 derived from 129P2/OlaHsd
Homozygous: very mild peripheral neuropathy with increased sensitivity to acrylamide, a neurotoxic agent that causes axon degeneration.
Heterozygous: no observable phenotype
Strain of Origin: 129P2/OlaHsd
Strain genetic background: B6.129P2
Strain Development: Lrsam1 mutant mice were created by using an existing gene trap BayGenomics ES cell line RRK461 (MMRRC:003459-UCD) available at MMRRC. Correctly targeted E14Tg2a.4 ES cells were injected into blastocysts. Offspring were backcrossed to C57BL/6J for at least 5 generations.
Suggested Control Mice: Wild-type littermates or C57BL/6J mice
Robert Burgess, Ph.D., The Jackson Laboratory.
Original ES cell line (MMRRC:003459) was donated by BayGenomics
Bogdanik LP, Sleigh JN, Tian C, Samuels ME, Bedard K, Seburn KL, Burgess RW. Loss of the E3 ubiquitin ligase LRSAM1 sensitizes peripheral axons to degeneration in a mouse model of Charcot-Marie-Tooth disease. Dis Model Mech. 2013 May-Jun;6(3):780-92. (Medline PMID: 23519028)
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email firstname.lastname@example.org.
Coat color: Black
MMRRC Breeding System: Backcross
Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Undetermined||Undetermined|
Average litter size: Not specified
Recommended wean age: 3-4 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.